For the best experience on the Abcam website please upgrade to a modern browser such as
Rabbit polyclonal to KPC2 - C-terminal
Predicted to work with:
Rat, Horse, Guinea pig, Dog, Chimpanzee, Rhesus monkey, Gorilla, Chinese hamster, Orangutan
Synthetic peptide within Human KPC2 aa 325-375 (C terminal). The exact sequence is proprietary. NP_057256.2
LLGDRK PSPEELDKGI DPDSPLFQAI LDNPVVQLGL TNPKTLLAFE DMLEN
293T, HeLa, Jurkat and TCMK1 whole cell lysates.
Shipped at 4°C. Store at +4°C short term (1-2 weeks). Upon delivery aliquot. Store at -20°C long term. Avoid freeze / thaw cycle.
Preservative: 0.09% Sodium azide
Constituent: 99% Tris citrate/phosphate pH 7 to 8
Concentration information loading...
Immunogen affinity purified
Abpromise guarantee covers the use of
in the following tested applications.
The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.
Use at 2-10 µg/mg of lysate.
1/2000 - 1/10000. Predicted molecular weight: 45 kDa.
KPC2 (Kip1 ubiquitylation-promoting complex 2) together with KPC1 forms the ubiquitin ligase KPC, which regulates degradation of the cyclin-dependent kinase inhibitor p27 at the G1 phase of the cell cycle. KPC2 contains a ubiquitin-like (UBL) domain, two ubiquitin-associated (UBA) domains, and a heat shock chaperonin-binding (STI1) domain.
E3 ubiquitin-protein ligase subunit KPC2 antibody
Western blot - Anti-KPC2 antibody - C-terminal (ab177519)
All lanes : Anti-KPC2 antibody - C-terminal (ab177519) at 0.1 µg/ml Lane 1 : 293T whole cell lysate Lane 2 : HeLa whole cell lysate Lane 3 : Jurkat whole cell lysate Lane 4 : TCMK1 whole cell lysate Lysates/proteins at 50 µg per lane. Secondary All lanes : Anti-rabbit HRP conjugated antibody Developed using the ECL technique. Predicted band size: 45 kDa Exposure time: 3 minutes
Immunoprecipitation - Anti-KPC2 antibody - C-terminal (ab177519)
All lanes : Anti-KPC2 antibody - C-terminal (ab177519) at 1 µg/ml Lane 1 : 293T whole cell lysate (1 mg for IP; 20% of IP loaded) immunoprecipitated using ab177519 at 6 µg/mg lysate. Lane 2 : 293T whole cell lysate (1 mg for IP; 20% of IP loaded) immunoprecipitated using Control IgG at 6 µg/mg lysate. Secondary All lanes : Anti-rabbit HRP conjugated antibody Developed using the ECL technique. Exposure time: 30 seconds
has not yet been referenced specifically in any publications.
Please note: All products are "FOR RESEARCH USE ONLY AND ARE NOT INTENDED FOR DIAGNOSTIC OR THERAPEUTIC USE"