Overview

  • Product name
    Anti-KPC2 antibody - C-terminal
    See all KPC2 primary antibodies
  • Description
    Rabbit polyclonal to KPC2 - C-terminal
  • Host species
    Rabbit
  • Tested applications
    Suitable for: IP, WBmore details
  • Species reactivity
    Reacts with: Mouse, Human
    Predicted to work with: Rat, Horse, Guinea pig, Dog, Chimpanzee, Rhesus monkey, Gorilla, Chinese hamster, Orangutan
  • Immunogen

    Synthetic peptide within Human KPC2 aa 325-375 (C terminal). The exact sequence is proprietary. NP_057256.2
    Sequence:

    LLGDRK PSPEELDKGI DPDSPLFQAI LDNPVVQLGL TNPKTLLAFE DMLEN


    Database link: Q9BSL1

  • Positive control
    • 293T, HeLa, Jurkat and TCMK1 whole cell lysates.

Properties

Applications

Our Abpromise guarantee covers the use of ab177519 in the following tested applications.

The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.

Application Abreviews Notes
IP Use at 2-10 µg/mg of lysate.
WB 1/2000 - 1/10000. Predicted molecular weight: 45 kDa.

Target

  • Relevance
    KPC2 (Kip1 ubiquitylation-promoting complex 2) together with KPC1 forms the ubiquitin ligase KPC, which regulates degradation of the cyclin-dependent kinase inhibitor p27 at the G1 phase of the cell cycle. KPC2 contains a ubiquitin-like (UBL) domain, two ubiquitin-associated (UBA) domains, and a heat shock chaperonin-binding (STI1) domain.
  • Cellular localization
    Cytoplasmic
  • Database links
  • Alternative names
    • 1110033G07Rik antibody
    • AA407978 antibody
    • E3 ubiquitin-protein ligase subunit KPC2 antibody
    • GBDR1 antibody
    • Glialblastoma cell differentiation-related protein 1 antibody
    • Glialblastoma differentiation-related protein 1 antibody
    • Kip1 ubiquitination-promoting complex protein 2 antibody
    • kpc 2 antibody
    • Kpc2 antibody
    • OTTHUMP00000022563 antibody
    • Putative glialblastoma cell differentiation-related protein antibody
    • RP11-432J22.3 antibody
    • UBA domain containing 1 antibody
    • UBA domain-containing protein 1 antibody
    • UBAC 1 antibody
    • UBAC1 antibody
    • Ubadc1 antibody
    • Ubiquitin associated domain containing 1 antibody
    • Ubiquitin-associated domain-containing protein 1 antibody
    see all

Images

  • All lanes : Anti-KPC2 antibody - C-terminal (ab177519) at 0.1 µg/ml

    Lane 1 : 293T whole cell lysate
    Lane 2 : HeLa whole cell lysate
    Lane 3 : Jurkat whole cell lysate
    Lane 4 : TCMK1 whole cell lysate

    Lysates/proteins at 50 µg per lane.

    Secondary
    All lanes : Anti-rabbit HRP conjugated antibody

    Developed using the ECL technique.

    Predicted band size: 45 kDa


    Exposure time: 3 minutes
  • All lanes : Anti-KPC2 antibody - C-terminal (ab177519) at 1 µg/ml

    Lane 1 : 293T whole cell lysate (1 mg for IP; 20% of IP loaded) immunoprecipitated using ab177519 at 6 µg/mg lysate.
    Lane 2 : 293T whole cell lysate (1 mg for IP; 20% of IP loaded) immunoprecipitated using Control IgG at 6 µg/mg lysate.

    Secondary
    All lanes : Anti-rabbit HRP conjugated antibody

    Developed using the ECL technique.

    Exposure time: 30 seconds

References

ab177519 has not yet been referenced specifically in any publications.

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Please note: All products are "FOR RESEARCH USE ONLY AND ARE NOT INTENDED FOR DIAGNOSTIC OR THERAPEUTIC USE"

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