Product nameAnti-KPNA2 antibody
See all KPNA2 primary antibodies
DescriptionChicken polyclonal to KPNA2
Tested applicationsSuitable for: WB, ICC/IFmore details
Species reactivityReacts with: Mouse, Rat, Human
Full length protein, corresponding to amino acids 1-529 of KPNA2
- KPNA2 recombinant protein.
Storage instructionsShipped at 4°C. Store at +4°C short term (1-2 weeks). Upon delivery aliquot. Store at -20°C long term.
Storage bufferConstituent: PBS
Concentration information loading...
PurityImmunogen affinity purified
Our Abpromise guarantee covers the use of ab37628 in the following tested applications.
The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.
|WB||1/2000. Predicted molecular weight: 58 kDa.|
|ICC/IF||Use at an assay dependent concentration. PubMed: 22384089|
FunctionFunctions in nuclear protein import as an adapter protein for nuclear receptor KPNB1. Binds specifically and directly to substrates containing either a simple or bipartite NLS motif. Docking of the importin/substrate complex to the nuclear pore complex (NPC) is mediated by KPNB1 through binding to nucleoporin FxFG repeats and the complex is subsequently translocated through the pore by an energy requiring, Ran-dependent mechanism. At the nucleoplasmic side of the NPC, Ran binds to importin-beta and the three components separate and importin-alpha and -beta are re-exported from the nucleus to the cytoplasm where GTP hydrolysis releases Ran from importin. The directionality of nuclear import is thought to be conferred by an asymmetric distribution of the GTP- and GDP-bound forms of Ran between the cytoplasm and nucleus.
Tissue specificityExpressed ubiquitously.
Sequence similaritiesBelongs to the importin alpha family.
Contains 10 ARM repeats.
Contains 1 IBB domain.
DomainConsists of an N-terminal hydrophilic region, a hydrophobic central region composed of 10 repeats, and a short hydrophilic C-terminus. The N-terminal hydrophilic region contains the importin beta binding domain (IBB domain), which is sufficient for binding importin beta and essential for nuclear protein import.
The IBB domain is thought to act as an intrasteric autoregulatory sequence by interacting with the internal autoinhibitory NLS. Binding of KPNB1 probably overlaps the internal NLS and contributes to a high affinity for cytoplasmic NLS-containing cargo substrates. After dissociation of the importin/substrate complex in the nucleus the internal autohibitory NLS contributes to a low affinity for nuclear NLS-containing proteins.
The major and minor NLS binding sites are mainly involved in recognition of simple or bipartite NLS motifs. Structurally located within in a helical surface groove they contain several conserved Trp and Asn residues of the corresponding third helices (H3) of ARM repeats which mainly contribute to binding.
Cellular localizationCytoplasm. Nucleus.
- Information by UniProt
- IMA1_HUMAN antibody
- Importin alpha 1 antibody
- Importin alpha 2 antibody
Anti-KPNA2 antibody (ab37628) at 1/2000 dilution + E coli-derived fusion protein at 2.5 µg
Goat anti-IgY-HRP dilution at 1/1000 dilution
Predicted band size: 58 kDa
Observed band size: 55 kDa why is the actual band size different from the predicted?
Predicted and actual molecular weights differ slightly due to the use of a fusion protein.
ab37628 has been referenced in 1 publication.
- Estrada-Bernal A et al. Functional complexity of the axonal growth cone: a proteomic analysis. PLoS One 7:e31858 (2012). WB, ICC/IF ; Rat . PubMed: 22384089