Recombinant
RabMAb

Anti-KPNA2 antibody [EPR11716(B)] (ab170495)

Overview

  • Product name
    Anti-KPNA2 antibody [EPR11716(B)]
    See all KPNA2 primary antibodies
  • Description
    Rabbit monoclonal [EPR11716(B)] to KPNA2
  • Host species
    Rabbit
  • Tested applications
    Suitable for: WB, Flow Cytmore details
    Unsuitable for: ICC,IHC-P or IP
  • Species reactivity
    Reacts with: Mouse, Rat, Human
  • Immunogen

    Synthetic peptide within Human KPNA2. The exact sequence is proprietary.
    Database link: P52292

  • Positive control
    • Permeabilized 293T cells; HeLa, HepG2, Jurkat, 293T and C6 cell lysates.
  • General notes

     

     

    Our RabMAb® technology is a patented hybridoma-based technology for making rabbit monoclonal antibodies. For details on our patents, please refer to RabMab® patents

    This product is a recombinant rabbit monoclonal antibody.

Properties

  • Form
    Liquid
  • Storage instructions
    Shipped at 4°C. Store at +4°C short term (1-2 weeks). Upon delivery aliquot. Store at -20°C long term. Avoid freeze / thaw cycle.
  • Storage buffer
    Preservative: 0.01% Sodium azide
    Constituents: 9% PBS, 40% Glycerol, 0.05% BSA, 50% Tissue culture supernatant
  • Purity
    Tissue culture supernatant
  • Clonality
    Monoclonal
  • Clone number
    EPR11716(B)
  • Isotype
    IgG
  • Research areas

Applications

Our Abpromise guarantee covers the use of ab170495 in the following tested applications.

The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.

Application Abreviews Notes
WB 1/10000 - 1/50000. Predicted molecular weight: 58 kDa.
Flow Cyt 1/10 - 1/100.

ab172730 - Rabbit monoclonal IgG, is suitable for use as an isotype control with this antibody.

 

  • Application notes
    Is unsuitable for ICC,IHC-P or IP.
  • Target

    • Function
      Functions in nuclear protein import as an adapter protein for nuclear receptor KPNB1. Binds specifically and directly to substrates containing either a simple or bipartite NLS motif. Docking of the importin/substrate complex to the nuclear pore complex (NPC) is mediated by KPNB1 through binding to nucleoporin FxFG repeats and the complex is subsequently translocated through the pore by an energy requiring, Ran-dependent mechanism. At the nucleoplasmic side of the NPC, Ran binds to importin-beta and the three components separate and importin-alpha and -beta are re-exported from the nucleus to the cytoplasm where GTP hydrolysis releases Ran from importin. The directionality of nuclear import is thought to be conferred by an asymmetric distribution of the GTP- and GDP-bound forms of Ran between the cytoplasm and nucleus.
    • Tissue specificity
      Expressed ubiquitously.
    • Sequence similarities
      Belongs to the importin alpha family.
      Contains 10 ARM repeats.
      Contains 1 IBB domain.
    • Domain
      Consists of an N-terminal hydrophilic region, a hydrophobic central region composed of 10 repeats, and a short hydrophilic C-terminus. The N-terminal hydrophilic region contains the importin beta binding domain (IBB domain), which is sufficient for binding importin beta and essential for nuclear protein import.
      The IBB domain is thought to act as an intrasteric autoregulatory sequence by interacting with the internal autoinhibitory NLS. Binding of KPNB1 probably overlaps the internal NLS and contributes to a high affinity for cytoplasmic NLS-containing cargo substrates. After dissociation of the importin/substrate complex in the nucleus the internal autohibitory NLS contributes to a low affinity for nuclear NLS-containing proteins.
      The major and minor NLS binding sites are mainly involved in recognition of simple or bipartite NLS motifs. Structurally located within in a helical surface groove they contain several conserved Trp and Asn residues of the corresponding third helices (H3) of ARM repeats which mainly contribute to binding.
    • Cellular localization
      Cytoplasm. Nucleus.
    • Information by UniProt
    • Database links
    • Alternative names
      • IMA1_HUMAN antibody
      • Importin alpha 1 antibody
      • Importin alpha 2 antibody
      • Importin alpha 2 subunit antibody
      • Importin alpha P1 antibody
      • Importin subunit alpha-1 antibody
      • IPO A1 antibody
      • IPOA 1 antibody
      • IPOA1 antibody
      • Karyopherin alpha 2 antibody
      • Karyopherin alpha 2 RAG cohort 1 antibody
      • Karyopherin alpha 2 subunit antibody
      • Karyopherin subunit alpha-2 antibody
      • KPNA2 antibody
      • KPNA2 protein antibody
      • Pendulin antibody
      • QIP 2 antibody
      • QIP2 antibody
      • RAG cohort 1 antibody
      • RAG cohort protein 1 antibody
      • RCH 1 antibody
      • RCH1 antibody
      • SRP 1 antibody
      • SRP1 alpha antibody
      • SRP1 antibody
      • SRP1-alpha antibody
      • SRP1alpha antibody
      see all

    Images

    • Flow cytometric analysis of permeabilized 293T cells labeling KPNA2 with ab170495 at 1/10 dilution (red)  or a rabbit IgG negative control (green).

    • All lanes : Anti-KPNA2 antibody [EPR11716(B)] (ab170495) at 1/10000 dilution

      Lane 1 : HeLa cell lysate
      Lane 2 : HepG2 cell lysate
      Lane 3 : Jurkat cell lysate
      Lane 4 : 293T cell lysate
      Lane 5 : C6 cell lysate

      Lysates/proteins at 10 µg per lane.

      Predicted band size: 58 kDa

    References

    ab170495 has not yet been referenced specifically in any publications.

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    Please note: All products are "FOR RESEARCH USE ONLY AND ARE NOT INTENDED FOR DIAGNOSTIC OR THERAPEUTIC USE"

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