• Product name

  • Description

    Rabbit polyclonal to KPNA3
  • Host species

  • Tested applications

    Suitable for: ICC/IF, IHC-Pmore details
  • Species reactivity

    Reacts with: Mouse, Human
    Predicted to work with: Chimpanzee, Rhesus monkey
  • Immunogen

    Synthetic peptide, corresponding to a region within the N terminal amino acids 1-50 of Human KPNA3 (accession number: NP_002258.2)

  • Positive control

    • Human anaplastic thyroid carcinoma, breast carcinoma, colon carcinoma, linitis plastica stomach cancer, ovarian carcinoma, prostate carcinoma, testicular seminoma.
  • General notes

    Antibody concentration was determined by extinction coefficient: absorbance at 280 nm of 1.4 equals 1.0 mg of IgG.



Our Abpromise guarantee covers the use of ab84706 in the following tested applications.

The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.

Application Abreviews Notes
  • Application notes
    ICC/IF: 1/100.
    IHC-P: 1/100 - 1/500. Epitope exposure is recommended. Epitope exposure with citrate buffer will enhance staining.

    Likely to work with frozen sections.

    Not yet tested in other applications.
    Optimal dilutions/concentrations should be determined by the end user.
  • Target

    • Function

      Functions in nuclear protein import as an adapter protein for nuclear receptor KPNB1. Binds specifically and directly to substrates containing either a simple or bipartite NLS motif. Docking of the importin/substrate complex to the nuclear pore complex (NPC) is mediated by KPNB1 through binding to nucleoporin FxFG repeats and the complex is subsequently translocated through the pore by an energy requiring, Ran-dependent mechanism. At the nucleoplasmic side of the NPC, Ran binds to importin-beta and the three components separate and importin-alpha and -beta are re-exported from the nucleus to the cytoplasm where GTP hydrolysis releases Ran from importin. The directionality of nuclear import is thought to be conferred by an asymmetric distribution of the GTP- and GDP-bound forms of Ran between the cytoplasm and nucleus. In vitro, mediates the nuclear import of human cytomegalovirus UL84 by recognizing a non-classical NLS. Recognizes NLSs of influenza A virus nucleoprotein probably through ARM repeats 7-9.
    • Tissue specificity

      Ubiquitous. Highest levels in heart and skeletal muscle.
    • Sequence similarities

      Belongs to the importin alpha family.
      Contains 10 ARM repeats.
      Contains 1 IBB domain.
    • Domain

      Consists of an N-terminal hydrophilic region, a hydrophobic central region composed of 10 repeats, and a short hydrophilic C-terminus. The N-terminal hydrophilic region contains the importin beta binding domain (IBB domain), which is sufficient for binding importin beta and essential for nuclear protein import.
      The IBB domain is thought to act as an intrasteric autoregulatory sequence by interacting with the internal autoinhibitory NLS. Binding of KPNB1 probably overlaps the internal NLS and contributes to a high affinity for cytoplasmic NLS-containing cargo substrates. After dissociation of the importin/substrate complex in the nucleus the internal autohibitory NLS contributes to a low affinity for nuclear NLS-containing proteins.
      The major and minor NLS binding sites are mainly involved in recognition of simple or bipartite NLS motifs. Structurally located within in a helical surface groove they contain several conserved Trp and Asn residues of the corresponding third helices (H3) of ARM repeats which mainly contribute to binding.
    • Cellular localization

      Cytoplasm. Nucleus.
    • Information by UniProt
    • Database links

    • Alternative names

      • SRP1 gamma antibody
      • hSRP1 antibody
      • IMA4_HUMAN antibody
      • Importin alpha 3 antibody
      • Importin alpha 3 subunit antibody
      • Importin alpha 4 antibody
      • Importin alpha Q2 antibody
      • Importin subunit alpha-4 antibody
      • IPOA4 antibody
      • Karyopherin alpha 3 antibody
      • Karyopherin alpha 3 importin alpha 4 antibody
      • Karyopherin alpha 3 subunit antibody
      • Karyopherin subunit alpha 3 antibody
      • Karyopherin subunit alpha-3 antibody
      • KPNA 3 antibody
      • Kpna3 antibody
      • Qip2 antibody
      • SRP1 antibody
      • SRP1-gamma antibody
      • SRP1gamma antibody
      • SRP4 antibody
      see all


    • Detection of Human KPNA3 by Immunohistochemistry.
      Sample: FFPE section of Human ovarian carcinoma.
      Antibody: ab84706 used at a dilution of 1/250.
      Detection: DAB staining.
    • Detection of Human KPNA3 by Immunohistochemistry.
      Sample: FFPE section of Human testis.
      Antibody: ab84706 used at a dilution of 1/500.
      Detection: DAB staining.
    • Detection of Mouse KPNA3 by Immunohistochemistry.
      Sample: FFPE section of Mouse teratoma.
      Antibody: ab84706 used at a dilution of 1/250.
      Detection: DAB staining.
    • Detection of Human KPNA3.
      Sample: FFPE section of Human colon carcinoma.
      Antibody: ab84706 used at a dilution of 1/100.
      Detection: Red-fluorescent goat anti-rabbit IgG highly cross-adsorbed Antibody Hilyte PlusTM 555 used at a dilution of 1/100.


    ab84706 has not yet been referenced specifically in any publications.

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