Overview

  • Product name

    Anti-KPNA3 antibody - C-terminal (Biotin)
    See all KPNA3 primary antibodies
  • Description

    Goat polyclonal to KPNA3 - C-terminal (Biotin)
  • Host species

    Goat
  • Conjugation

    Biotin
  • Tested applications

    Suitable for: WBmore details
  • Species reactivity

    Reacts with: Rat
    Predicted to work with: Mouse, Human
  • Immunogen

    Synthetic peptide corresponding to Human KPNA3 aa 509-521 (C terminal) (Cysteine residue). (NP_002258.2)
    Sequence:

    (c)DPTANLQTKEFNF


    Database link: O00505

  • Positive control

    • WB: Rat testis lysate.

Properties

  • Form

    Liquid
  • Storage instructions

    Shipped at 4°C. Store at +4°C short term (1-2 weeks). Upon delivery aliquot. Store at -20°C long term. Avoid freeze / thaw cycle. Store In the Dark.
  • Storage buffer

    pH: 7.30
    Preservative: 0.02% Sodium azide
    Constituents: Tris buffered saline, 0.5% BSA
  • Concentration information loading...
  • Purity

    Immunogen affinity purified
  • Purification notes

    Purified from goat serum by ammonium sulphate precipitation followed by antigen affinity chromatography using the immunizing peptide.
  • Clonality

    Polyclonal
  • Isotype

    IgG
  • Research areas

Applications

Our Abpromise guarantee covers the use of ab240463 in the following tested applications.

The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.

Application Abreviews Notes
WB Use a concentration of 1 - 3 µg/ml. Predicted molecular weight: 57 kDa.

Target

  • Function

    Functions in nuclear protein import as an adapter protein for nuclear receptor KPNB1. Binds specifically and directly to substrates containing either a simple or bipartite NLS motif. Docking of the importin/substrate complex to the nuclear pore complex (NPC) is mediated by KPNB1 through binding to nucleoporin FxFG repeats and the complex is subsequently translocated through the pore by an energy requiring, Ran-dependent mechanism. At the nucleoplasmic side of the NPC, Ran binds to importin-beta and the three components separate and importin-alpha and -beta are re-exported from the nucleus to the cytoplasm where GTP hydrolysis releases Ran from importin. The directionality of nuclear import is thought to be conferred by an asymmetric distribution of the GTP- and GDP-bound forms of Ran between the cytoplasm and nucleus. In vitro, mediates the nuclear import of human cytomegalovirus UL84 by recognizing a non-classical NLS. Recognizes NLSs of influenza A virus nucleoprotein probably through ARM repeats 7-9.
  • Tissue specificity

    Ubiquitous. Highest levels in heart and skeletal muscle.
  • Sequence similarities

    Belongs to the importin alpha family.
    Contains 10 ARM repeats.
    Contains 1 IBB domain.
  • Domain

    Consists of an N-terminal hydrophilic region, a hydrophobic central region composed of 10 repeats, and a short hydrophilic C-terminus. The N-terminal hydrophilic region contains the importin beta binding domain (IBB domain), which is sufficient for binding importin beta and essential for nuclear protein import.
    The IBB domain is thought to act as an intrasteric autoregulatory sequence by interacting with the internal autoinhibitory NLS. Binding of KPNB1 probably overlaps the internal NLS and contributes to a high affinity for cytoplasmic NLS-containing cargo substrates. After dissociation of the importin/substrate complex in the nucleus the internal autohibitory NLS contributes to a low affinity for nuclear NLS-containing proteins.
    The major and minor NLS binding sites are mainly involved in recognition of simple or bipartite NLS motifs. Structurally located within in a helical surface groove they contain several conserved Trp and Asn residues of the corresponding third helices (H3) of ARM repeats which mainly contribute to binding.
  • Cellular localization

    Cytoplasm. Nucleus.
  • Information by UniProt
  • Database links

  • Alternative names

    • SRP1 gamma antibody
    • hSRP1 antibody
    • IMA4_HUMAN antibody
    • Importin alpha 3 antibody
    • Importin alpha 3 subunit antibody
    • Importin alpha 4 antibody
    • Importin alpha Q2 antibody
    • Importin subunit alpha-4 antibody
    • IPOA4 antibody
    • Karyopherin alpha 3 antibody
    • Karyopherin alpha 3 importin alpha 4 antibody
    • Karyopherin alpha 3 subunit antibody
    • Karyopherin subunit alpha 3 antibody
    • Karyopherin subunit alpha-3 antibody
    • KPNA 3 antibody
    • Kpna3 antibody
    • Qip2 antibody
    • SRP1 antibody
    • SRP1-gamma antibody
    • SRP1gamma antibody
    • SRP4 antibody
    see all

Images

  • Anti-KPNA3 antibody - C-terminal (Biotin) (ab240463) at 1 µg/ml + Rat testis lysate in RIPA buffer at 35 µg

    Predicted band size: 57 kDa



    Primary incubation for 1 hour.

    Detected by chemiluminescence, using streptavidin-HRP and using NAP blocker as a substitute for skimmed milk.

     

References

ab240463 has not yet been referenced specifically in any publications.

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