Key features and details
- Goat polyclonal to KPNA4
- Suitable for: IHC-P, WB
- Reacts with: Mouse, Human
- Isotype: IgG
Product nameAnti-KPNA4 antibody
See all KPNA4 primary antibodies
DescriptionGoat polyclonal to KPNA4
Tested applicationsSuitable for: IHC-P, WBmore details
Species reactivityReacts with: Mouse, Human
Predicted to work with: Rat, Cow, Dog
- Recombinant Human KPNA4 protein (ab114626) can be used as a positive control in WB. Human testis and ovary lysate. Human testis tissue sections and human small intestine sections.
General notesGenBank Accession Number – NP_002259.
The nuclear import of karyophilic proteins is directed by short amino acid sequences termed nuclear localization signals (NLSs). Karyopherins, or importins, are cytoplasmic proteins that recognize NLSs and dock NLS-containing proteins to the nuclear pore complex. The protein encoded by this gene shares the sequence similarity with Xenopus importin-alpha and Saccharomyces cerevisiae Srp1. This protein is found to interact with the NLSs of DNA helicase Q1 and SV40 T antigen.
Storage instructionsShipped at 4°C. Upon delivery aliquot and store at -20°C or -80°C. Avoid repeated freeze / thaw cycles.
Storage bufferpH: 7.30
Preservative: 0.02% Sodium azide
Constituents: Tris buffered saline, 0.5% BSA
Concentration information loading...
PurityImmunogen affinity purified
Purification notesPurified from goat serum by ammonium sulphate precipitation followed by antigen affinity chromatography using the immunizing peptide.
Primary antibody notesThe nuclear import of karyophilic proteins is directed by short amino acid sequences termed nuclear localization signals (NLSs). Karyopherins, or importins, are cytoplasmic proteins that recognize NLSs and dock NLS-containing proteins to the nuclear pore complex. The protein encoded by this gene shares the sequence similarity with Xenopus importin-alpha and Saccharomyces cerevisiae Srp1. This protein is found to interact with the NLSs of DNA helicase Q1 and SV40 T antigen.
Our Abpromise guarantee covers the use of ab6039 in the following tested applications.
The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.
|IHC-P||Use a concentration of 1 - 2 µg/ml.|
|WB||Use a concentration of 0.2 - 1 µg/ml. Detects a band of approximately 55-60 kDa (predicted molecular weight: 58 kDa).
A 1 hour primary incubation is recommended for this product. A customer reported that milk can significantly suppress antigen-antibody interaction on nitrocellulose membrane in WB.
FunctionFunctions in nuclear protein import as an adapter protein for nuclear receptor KPNB1. Binds specifically and directly to substrates containing either a simple or bipartite NLS motif. Docking of the importin/substrate complex to the nuclear pore complex (NPC) is mediated by KPNB1 through binding to nucleoporin FxFG repeats and the complex is subsequently translocated through the pore by an energy requiring, Ran-dependent mechanism. At the nucleoplasmic side of the NPC, Ran binds to importin-beta and the three components separate and importin-alpha and -beta are re-exported from the nucleus to the cytoplasm where GTP hydrolysis releases Ran from importin. The directionality of nuclear import is thought to be conferred by an asymmetric distribution of the GTP- and GDP-bound forms of Ran between the cytoplasm and nucleus. In vitro, mediates the nuclear import of human cytomegalovirus UL84 by recognizing a non-classical NLS. In vitro, mediates the nuclear import of human cytomegalovirus UL84 by recognizing a non-classical NLS.
Tissue specificityHighly expressed in testis, ovary, small intestine, heart, skeletal muscle, lung and pancreas, but barely detectable in kidney, thymus, colon and peripheral blood leukocytes.
Sequence similaritiesBelongs to the importin alpha family.
Contains 10 ARM repeats.
Contains 1 IBB domain.
DomainConsists of an N-terminal hydrophilic region, a hydrophobic central region composed of 10 repeats, and a short hydrophilic C-terminus. The N-terminal hydrophilic region contains the importin beta binding domain (IBB domain), which is sufficient for binding importin beta and essential for nuclear protein import.
The IBB domain is thought to act as an intrasteric autoregulatory sequence by interacting with the internal autoinhibitory NLS. Binding of KPNB1 probably overlaps the internal NLS and contributes to a high affinity for cytoplasmic NLS-containing cargo substrates. After dissociation of the importin/substrate complex in the nucleus the internal autohibitory NLS contributes to a low affinity for nuclear NLS-containing proteins.
The major and minor NLS binding sites are mainly involved in recognition of simple or bipartite NLS motifs. Structurally located within in a helical surface groove they contain several conserved Trp and Asn residues of the corresponding third helices (H3) of ARM repeats which mainly contribute to binding.
Cellular localizationCytoplasm. Nucleus.
- Information by UniProt
- FLJ31113 antibody
- IMA3_HUMAN antibody
- Importin alpha 3 antibody
All lanes : Anti-KPNA4 antibody (ab6039) at 1 µg/ml
Lane 1 : Human testis
Lane 2 : Human ovary
Lane 3 : Mouse testis
Lane 4 : Rat testis
Lane 5 : Rat ovary
Lysates/proteins at 35 µg per lane.
Predicted band size: 58 kDa
Immunohistochemical analysis of 10% buffered formalin-fixed paraffin-embedded murine nephric tissue sections, labelling KPNA4 with ab6039 at a dilution of 1/100 incubated for 12 hours at 4°C. Heat mediated antigen retrival was performed through 10mM citrate buffer at pH 6.0. Blocking was with 5% serum incubated for 1 hour at 21°C. Secondary was a polyclonal Donkey anti-Goat Alexa Fluor® 647 conjugate at 1/200. Counterstain is DAPI against nuclear DNA. The Negative control is the same as above, but in the abscence of the primary ab6039.
ab6039 at 0.5
µg/ml staining approximately 55-60kDa band of KPNA4 in Human Testis lysate by Western blot (ECL). ab6039 at 0.5µg/ml staining approximately 55-60kDa band of KPNA4 in Human Testis lysate by Western blot (ECL).
ab6039 (3µg/ml) staining Human Testis by IHC-P. Microwaved antigen retrieval with citrate buffer (pH 6), HRP-staining.
ab6039 at 5µg/ml staining KPNA4 in human small intestinal tissue by Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections). Steamed antigen retrieval with citrate buffer pH 6, AP-staining.
ab6039 has been referenced in 14 publications.
- Guo L et al. Phosphorylation of importin-a1 by CDK1-cyclin B1 controls mitotic spindle assembly. J Cell Sci 132:N/A (2019). PubMed: 31434716
- Zheng Y et al. MicroRNA-24 inhibits the proliferation and migration of endothelial cells in patients with atherosclerosis by targeting importin-a3 and regulating inflammatory responses. Exp Ther Med 15:338-344 (2018). PubMed: 29250154
- Major AT et al. Development of a pipeline for automated, high-throughput analysis of paraspeckle proteins reveals specific roles for importin a proteins. Sci Rep 7:43323 (2017). PubMed: 28240251
- Audsley MD et al. Nucleocytoplasmic trafficking of Nipah virus W protein involves multiple discrete interactions with the nuclear import and export machinery. Biochem Biophys Res Commun 479:429-433 (2016). IP ; Human . PubMed: 27622322
- De Houwer S et al. The HIV-1 integrase mutant R263A/K264A is 2-fold defective for TRN-SR2 binding and viral nuclear import. J Biol Chem 289:25351-61 (2014). WB ; Human . PubMed: 25063804