Overview

  • Product name

  • Description

    Rabbit polyclonal to KPNA4
  • Host species

    Rabbit
  • Tested applications

    Suitable for: ICC/IF, IHC-Pmore details
  • Species reactivity

    Reacts with: Mouse, Human
    Predicted to work with: Rabbit, Horse, Guinea pig, Cow, Dog, Chimpanzee, Rhesus monkey, Gorilla, Chinese hamster, Orangutan, Elephant
  • Immunogen

    Synthetic peptide corresponding to a region within Human KPNA4

Properties

  • Form

    Liquid
  • Storage instructions

    Shipped at 4°C. Upon delivery aliquot and store at -20°C. Avoid freeze / thaw cycles.
  • Storage buffer

    Preservative: 0.09% Sodium azide
    Constituents: 0.1% BSA, Tris buffered saline
  • Concentration information loading...
  • Purity

    Immunogen affinity purified
  • Clonality

    Polyclonal
  • Isotype

    IgG
  • Research areas

Applications

Our Abpromise guarantee covers the use of ab84735 in the following tested applications.

The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.

Application Abreviews Notes
ICC/IF Use at an assay dependent concentration.
IHC-P 1/250.

Target

  • Function

    Functions in nuclear protein import as an adapter protein for nuclear receptor KPNB1. Binds specifically and directly to substrates containing either a simple or bipartite NLS motif. Docking of the importin/substrate complex to the nuclear pore complex (NPC) is mediated by KPNB1 through binding to nucleoporin FxFG repeats and the complex is subsequently translocated through the pore by an energy requiring, Ran-dependent mechanism. At the nucleoplasmic side of the NPC, Ran binds to importin-beta and the three components separate and importin-alpha and -beta are re-exported from the nucleus to the cytoplasm where GTP hydrolysis releases Ran from importin. The directionality of nuclear import is thought to be conferred by an asymmetric distribution of the GTP- and GDP-bound forms of Ran between the cytoplasm and nucleus. In vitro, mediates the nuclear import of human cytomegalovirus UL84 by recognizing a non-classical NLS. In vitro, mediates the nuclear import of human cytomegalovirus UL84 by recognizing a non-classical NLS.
  • Tissue specificity

    Highly expressed in testis, ovary, small intestine, heart, skeletal muscle, lung and pancreas, but barely detectable in kidney, thymus, colon and peripheral blood leukocytes.
  • Sequence similarities

    Belongs to the importin alpha family.
    Contains 10 ARM repeats.
    Contains 1 IBB domain.
  • Domain

    Consists of an N-terminal hydrophilic region, a hydrophobic central region composed of 10 repeats, and a short hydrophilic C-terminus. The N-terminal hydrophilic region contains the importin beta binding domain (IBB domain), which is sufficient for binding importin beta and essential for nuclear protein import.
    The IBB domain is thought to act as an intrasteric autoregulatory sequence by interacting with the internal autoinhibitory NLS. Binding of KPNB1 probably overlaps the internal NLS and contributes to a high affinity for cytoplasmic NLS-containing cargo substrates. After dissociation of the importin/substrate complex in the nucleus the internal autohibitory NLS contributes to a low affinity for nuclear NLS-containing proteins.
    The major and minor NLS binding sites are mainly involved in recognition of simple or bipartite NLS motifs. Structurally located within in a helical surface groove they contain several conserved Trp and Asn residues of the corresponding third helices (H3) of ARM repeats which mainly contribute to binding.
  • Cellular localization

    Cytoplasm. Nucleus.
  • Information by UniProt
  • Database links

  • Alternative names

    • FLJ31113 antibody
    • IMA3_HUMAN antibody
    • Importin alpha 3 antibody
    • Importin alpha 4 subunit antibody
    • Importin alpha Q1 antibody
    • Importin subunit alpha 3 antibody
    • Importin subunit alpha-3 antibody
    • IPO A3 antibody
    • IPOA 3 antibody
    • IPOA3 antibody
    • Karyopherin alpha 4 (importin alpha 3) antibody
    • Karyopherin alpha 4 antibody
    • Karyopherin alpha 4 importin alpha 3 antibody
    • Karyopherin alpha 4 subunit antibody
    • Karyopherin subunit alpha 4 antibody
    • Karyopherin subunit alpha-4 antibody
    • KPNA 4 antibody
    • Kpna4 antibody
    • MGC12217 antibody
    • MGC26703 antibody
    • OTTHUMP00000213880 antibody
    • QIP 1 antibody
    • Qip1 antibody
    • Qip1 protein antibody
    • SRP 3 antibody
    • SRP3 antibody
    see all

Images

  • FFPE section of human pancreatic islet cell tumor using ab84735 at a dilution of 1:250. Detection: DAB staining.

References

ab84735 has not yet been referenced specifically in any publications.

Customer reviews and Q&As

1-2 of 2 Abreviews or Q&A

Answer

Thank you for contacting us.


I can confirm that the epitope recognized by this antibody (ab84735) maps to a region between residue 471 and 521 of human karyopherin alpha 4 using the numbering given in entry NP_002259.1 (GeneID 3840).

I hope this information is helpful to you. Please do not hesitate to contact us if you need any more advice or information.

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Answer

Vielen Dank für Ihre Anfrage und Ihre Geduld.

Ich habe mir unseren Katalog einmal angesehen und mit mich meinen Kollegen aus dem Labor beraten (daher die Verzögerung), und ich möchte Ihnen die folgenden Antikörper empfehlen:

https://www.abcam.com/index.html?datasheet=70160 (or use the following: https://www.abcam.com/index.html?datasheet=70160).

https://www.abcam.com/index.html?datasheet=84735 (or use the following: https://www.abcam.com/index.html?datasheet=84735).

Beide Antikörper sind sowohl in der Spezies als auch in der Applikation getestet. Des Weiteren stammt das Immunogen des ab70160 aus dem C-Terminus, und die Identitäten des Immunogens mit dem KPNA4 beträgt 42%. Damit ein Antikörper kreuzreagiert, sollte die Übereinstimmung mindestens 85% betragen. Es daher unwahrscheinlich, dass der Antikörper KPNA4 detektieren wird.

Das Immunogen des Anti-KPNA4 ab84735 des zeigt ebenfalls eine zu geringe Übereinstimmung mit dem KPNA2 Protein, und sollte daher spezifisch mit dem eigentlichen Zielprotein reagieren.

Ich hoffe, dies hilft Ihnen weiter. Bitte zögern Sie nicht, sich wieder bei uns zu melden, falls Sie weitere Fragen haben.

Benutzen Sie unsere Produkte? Schicken Sie uns einen Abreview. Verdienen Sie sich eine Belohnung!
https://www.abcam.com/abreviews

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