Anti-KPNA6 antibody (ab105350)
Key features and details
- Rabbit polyclonal to KPNA6
- Suitable for: ICC/IF, WB
- Reacts with: Human
- Isotype: IgG
Overview
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Product name
Anti-KPNA6 antibody -
Description
Rabbit polyclonal to KPNA6 -
Host species
Rabbit -
Specificity
ab105350 is predicted to not crossreact with other Importin alpha family members. -
Tested applications
Suitable for: ICC/IF, WBmore details -
Species reactivity
Reacts with: Human -
Immunogen
A 13 amino acid peptide from a region within the N terminus of Human KPNA6 (CAH71948).
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Positive control
- 293 cell lysate
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General notes
The Life Science industry has been in the grips of a reproducibility crisis for a number of years. Abcam is leading the way in addressing this with our range of recombinant monoclonal antibodies and knockout edited cell lines for gold-standard validation. Please check that this product meets your needs before purchasing.
If you have any questions, special requirements or concerns, please send us an inquiry and/or contact our Support team ahead of purchase. Recommended alternatives for this product can be found below, along with publications, customer reviews and Q&As
Properties
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Form
Liquid -
Storage instructions
Shipped at 4°C. Store at +4°C. -
Storage buffer
pH: 7.2
Preservative: 0.02% Sodium azide
Constituent: PBS -
Concentration information loading...
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Purity
Immunogen affinity purified -
Clonality
Polyclonal -
Isotype
IgG -
Research areas
Associated products
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Compatible Secondaries
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Isotype control
Applications
The Abpromise guarantee
Our Abpromise guarantee covers the use of ab105350 in the following tested applications.
The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.
Application | Abreviews | Notes |
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ICC/IF |
Use a concentration of 20 µg/ml.
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WB |
Use a concentration of 1 - 2 µg/ml. Predicted molecular weight: 60 kDa.
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Notes |
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ICC/IF
Use a concentration of 20 µg/ml. |
WB
Use a concentration of 1 - 2 µg/ml. Predicted molecular weight: 60 kDa. |
Target
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Function
Functions in nuclear protein import as an adapter protein for nuclear receptor KPNB1. Binds specifically and directly to substrates containing either a simple or bipartite NLS motif. Docking of the importin/substrate complex to the nuclear pore complex (NPC) is mediated by KPNB1 through binding to nucleoporin FxFG repeats and the complex is subsequently translocated through the pore by an energy requiring, Ran-dependent mechanism. At the nucleoplasmic side of the NPC, Ran binds to importin-beta and the three components separate and importin-alpha and -beta are re-exported from the nucleus to the cytoplasm where GTP hydrolysis releases Ran from importin. The directionality of nuclear import is thought to be conferred by an asymmetric distribution of the GTP- and GDP-bound forms of Ran between the cytoplasm and nucleus. -
Tissue specificity
Widely expressed. -
Sequence similarities
Belongs to the importin alpha family.
Contains 10 ARM repeats.
Contains 1 IBB domain. - Information by UniProt
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Database links
- Entrez Gene: 23633 Human
- Omim: 610563 Human
- SwissProt: O60684 Human
- Unigene: 470588 Human
- Unigene: 711019 Human
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Form
Cellular location: cytoplasm; nucleus. -
Alternative names
- FLJ11249 antibody
- IMA7_HUMAN antibody
- Importin alpha 7 subunit antibody
see all
Images
Protocols
Datasheets and documents
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SDS download
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Datasheet download
References (1)
ab105350 has been referenced in 1 publication.
- Marathe S et al. Notch signaling in response to excitotoxicity induces neurodegeneration via erroneous cell cycle reentry. Cell Death Differ 22:1775-84 (2015). PubMed: 25822340