Western blot abreview for Anti-KPNB1 antibody [3E9]

Excellent
Abreviews
Application
Western blot
Sample
Dog Cell lysate - whole cell (MDCK cells)
Gel Running Conditions
Reduced Denaturing (8% Tris-Glycine gel)
Loading amount
100 µg
Specification
MDCK cells
Blocking step
Milk as blocking agent for 30 minute(s) · Concentration: 5% · Temperature: 22°C

Other product details

Incubation time
2 hour(s) and 0 minute(s) · Temperature: 22°C · Diluent: 5% milk in TBS-T (0.1%)
Dilution
1/1000

Secondary antibody

Dilution
1/5000
Name
Non-Abcam antibody was used: Goat anti-Mouse IgG (H+L)-HRP Conjugate
Host species: Goat
Clonality: Polyclonal
Conjugation: Horse Radish Peroxidase

Detection

Detection method
ECL pico
Exposure
2 second(s)
Bands
Specific: 97 kDa
Positive control
MDCK cell lysate

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Submitted May 14 2013

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