Western blot abreview for Anti-KPNB1 antibody [3E9]

Excellent
Abreviews
Application
Western blot
Sample
Human Cell lysate - whole cell (HEK-293F and HeLa cells)
Gel Running Conditions
Reduced Denaturing (10)
Loading amount
75 µg
Specification
HEK-293F and HeLa cells
Blocking step
Milk as blocking agent for 30 minute(s) · Concentration: 5% · Temperature: 25°C

Other product details

Incubation time
16 hour(s) and 0 minute(s) · Temperature: 4°C · Diluent: TBS-T+5% milk
Dilution
1/2000

Secondary antibody

Dilution
1/5000
Name
Non-Abcam antibody was used: Goat anti-mouse IgG HRP linked
Host species: Goat
Clonality: Polyclonal
Conjugation: Horse Radish Peroxidase

Detection

Detection method
ECL+
Exposure
2 minute(s) and 0 second(s)
Bands
Specific: 97 kDa
Positive control
HEK-293F and HeLa cell lysates

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Submitted Oct 17 2013

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