Western blot abreview for Anti-KPNB1 antibody [3E9]

Excellent
Abreviews
Application
Western blot
Sample
Cow Cell lysate - whole cell (MDBK)
Gel Running Conditions
Reduced Denaturing (10)
Loading amount
50 µg
Specification
MDBK
Blocking step
Milk as blocking agent for 30 minute(s) · Concentration: 5% · Temperature: 25°C

Other product details

Incubation time
16 hour(s) and 0 minute(s) · Temperature: 4°C · Diluent: TBS-T with 5% milk
Dilution
1/2000

Secondary antibody

Dilution
1/5000
Name
Non-Abcam antibody was used: Goat anti-mouse IgG HRP
Host species: Goat
Clonality: Polyclonal
Conjugation: Horse Radish Peroxidase

Detection

Detection method
ECL+
Exposure
5 second(s)
Bands
Specific: 97 kDa
Positive control
MDBK cell lysate

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Submitted Oct 28 2013

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