Immunocytochemistry/ Immunofluorescence abreview for Anti-KPNB1 antibody [3E9]

Excellent
Abreviews
Application
Immunocytochemistry/ Immunofluorescence
Sample
Human Cell (HeLa)
Permeabilization
Yes - 0.5% Triton X-100 in PBS
Specification
HeLa
Blocking step
BSA as blocking agent for 1 hour(s) and 0 minute(s) · Concentration: 1% · Temperature: 20°C
Fixative
Paraformaldehyde

Other product details

Incubation time
45 minute(s) · Temperature: 20°C · Diluent: PBS + 0.1% Tween 20 + 0.1% BSA
Dilution
1/800

Secondary antibody

Dilution
1/1000

Additional data

Additional Notes
Enriched at the nuclear periphery similar to transiently expressed mCherry tagged protein.

Dr. Andrew Bowman

Verified customer

Submitted Apr 10 2017

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