Overview

  • Product name

  • Description

    Rabbit polyclonal to KPNB1
  • Host species

    Rabbit
  • Tested applications

    Suitable for: IP, WBmore details
  • Species reactivity

    Reacts with: Mouse, Human
    Predicted to work with: Rat
  • Immunogen

    Synthetic peptide within Human KPNB1 aa 825-876. The exact sequence is proprietary. (NP_002256.2).
    Database link: Q14974

  • Positive control

    • WB: HeLa, HEK-293T and NIH/3T3 whole cell lysates. IP: HeLa whole cell lysate.

Properties

Applications

Our Abpromise guarantee covers the use of ab245402 in the following tested applications.

The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.

Application Abreviews Notes
IP Use at 2-5 µg/mg of lysate.
WB 1/5000 - 1/15000. Predicted molecular weight: 97 kDa.

Target

  • Function

    Functions in nuclear protein import, either in association with an adapter protein, like an importin-alpha subunit, which binds to nuclear localization signals (NLS) in cargo substrates, or by acting as autonomous nuclear transport receptor. Acting autonomously, serves itself as NLS receptor. Docking of the importin/substrate complex to the nuclear pore complex (NPC) is mediated by KPNB1 through binding to nucleoporin FxFG repeats and the complex is subsequently translocated through the pore by an energy requiring, Ran-dependent mechanism. At the nucleoplasmic side of the NPC, Ran binds to importin-beta and the three components separate and importin-alpha and -beta are re-exported from the nucleus to the cytoplasm where GTP hydrolysis releases Ran from importin. The directionality of nuclear import is thought to be conferred by an asymmetric distribution of the GTP- and GDP-bound forms of Ran between the cytoplasm and nucleus. Mediates autonomously the nuclear import of ribosomal proteins RPL23A, RPS7 and RPL5. Binds to a beta-like import receptor binding (BIB) domain of RPL23A. In association with IPO7 mediates the nuclear import of H1 histone. In vitro, mediates nuclear import of H2A, H2B, H3 and H4 histones. In case of HIV-1 infection, binds and mediates the nuclear import of HIV-1 Rev. Imports PRKCI into the nucleus.
  • Sequence similarities

    Belongs to the importin beta family.
    Contains 8 HEAT repeats.
    Contains 1 importin N-terminal domain.
  • Cellular localization

    Cytoplasm. Nucleus envelope.
  • Information by UniProt
  • Database links

  • Alternative names

    • IMB 1 antibody
    • IMB1 antibody
    • IMB1_HUMAN antibody
    • Impnb antibody
    • Importin 90 antibody
    • Importin beta 1 antibody
    • Importin beta 1 subunit antibody
    • Importin subunit beta-1 antibody
    • Importin-90 antibody
    • IPOB antibody
    • Karyopherin beta 1 antibody
    • Karyopherin beta 1 subunit antibody
    • Karyopherin subunit beta-1 antibody
    • KPNB 1 antibody
    • Kpnb1 antibody
    • MGC2155 antibody
    • MGC2156 antibody
    • MGC2157 antibody
    • NTF 97 antibody
    • NTF97 antibody
    • NTF97/Importin beta antibody
    • Nuclear factor P97 antibody
    • Pore targeting complex 97 kDa subunit antibody
    • PTAC97 antibody
    see all

Images

  • All lanes : Anti-KPNB1 antibody (ab245402) at 0.1 µg/ml

    Lane 1 : HeLa (human epithelial cell line from cervix adenocarcinoma) whole cell lysate
    Lane 2 : HEK-293T (human epithelial cell line from embryonic kidney transformed with large T antigen) whole cell lysate
    Lane 3 : NIH/3T3 (mouse embryo fibroblast cell line) whole cell lysate

    Lysates/proteins at 50 µg per lane.

    Developed using the ECL technique.

    Predicted band size: 97 kDa


    Exposure time: 30 seconds
  • KPNB1 was immunoprecipitated from HeLa (human epithelial cell line from cervix adenocarcinoma) whole cell lysate (1 mg for IP, 25% of IP loaded) with ab245402 at 3 µg/mg lysate. Western blot was performed from the immunoprecipitate using ab245402 at 0.1 µg/ml.

    Lane 1: ab245402 IP in HeLa whole cell lysate.
    Lane 2: Control IgG IP in HeLa whole cell lysate.

    Detection: Chemiluminescence with exposure time of 30 seconds.

References

ab245402 has not yet been referenced specifically in any publications.

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