For the best experience on the Abcam website please upgrade to a modern browser such as Google Chrome

We use cookies to make our site as useful as possible.

Our Cookie Policy explains how you can opt-out of the cookies we use.

If you continue without changing your cookie settings, we'll assume you’re happy with this.

Continue Continue

United States
Your country/region is currently set to:

If incorrect, please enter your country/region into the box below, to view site information related to your country/region.

Call (888) 77-ABCAM (22226) or contact us
Need help? Contact us

  • My account
  • Sign out
Sign in or Register with us

Welcome

Sign in or

Don't have an account?

Register with us
My basket
Quick order
Abcam homepage

  • Research Products
    By product type
    Primary antibodies
    Secondary antibodies
    ELISA and Matched Antibody Pair Kits
    Cell and tissue imaging tools
    Cellular and biochemical assays
    Proteins and Peptides
    By product type
    Proteomics tools
    Agonists, activators, antagonists and inhibitors
    Cell lines and Lysates
    Multiplex miRNA assays
    Multiplex Assays
    By research area
    Cancer
    Cardiovascular
    Cell Biology
    Epigenetics
    Metabolism
    Developmental Biology
    By research area
    Immunology
    Microbiology
    Neuroscience
    Signal Transduction
    Stem Cells
  • Diagnostic & Therapeutic Solutions
    Custom solutions & partnerships

    Custom antibody development and commercial partnerships to advance your diagnostic and therapeutic discovery.

    Create custom solutions with us

    Partner with us

  • Support
    Support hub

    Access advice and support for any research roadblock

    View support hub

    Protocols

    Your experiments laid out step by step

    View protocols

  • Events
    • Conference calendar
    • Cancer
    • Cardiovascular
    • Epigenetics & Nuclear signaling
    • Immunology
    • Neuroscience
    • Stem cells
    • Tradeshows
    • Scientific webinars
    Keep up to date with the latest events

    Full event breakdown with abstracts, speakers, registration and more

    View global event calendar

  • Pathways
    Cell signalling pathways

    View all pathways

    View all interactive pathways

Supporting our customers and employees during the COVID-19 pandemic. Read more

  1. Link

    kpnb1-antibody-ab45938.pdf

  1. Send me a copy of this email
    I agree to the terms and conditions.
Tags & Cell Markers Subcellular Markers Nucleus Nuclear Envelope
Share by email

Anti-KPNB1 antibody (ab45938)

  • Datasheet
Reviews (1)Q&A (1)References (7)

Product price, shipping and contact information

Currently unavailable

Sorry, we can't display this right now.

Please contact us to place your order, or try again later.

 

Loading size & price…

 

Shipping and order information

Abpromise

Guaranteed product quality, expert customer support.

Find out more.

Western blot - Anti-KPNB1 antibody (ab45938)
  • Immunoprecipitation - Anti-KPNB1 antibody (ab45938)
  • Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-KPNB1 antibody (ab45938)

Key features and details

  • Rabbit polyclonal to KPNB1
  • Suitable for: IP, IHC-P, WB
  • Reacts with: Mouse, Rat, Human
  • Isotype: IgG

You may also be interested in

Cocktail
Product image
Membrane Fraction WB Cocktail (ab140365)
Secondary
Product image
Goat Anti-Rabbit IgG H&L (HRP) (ab205718)
Primary
Product image
Anti-EGFR antibody [EP38Y] (ab52894)

View more associated products

Overview

  • Product name

    Anti-KPNB1 antibody
    See all KPNB1 primary antibodies
  • Description

    Rabbit polyclonal to KPNB1
  • Host species

    Rabbit
  • Tested Applications & Species

    Application Species
    IHC-P
    Human
    IP
    Human
    WB
    Human
    See all applications and species data
  • Immunogen

    Synthetic peptide corresponding to Human KPNB1 aa 50-150 conjugated to keyhole limpet haemocyanin.
    (Peptide available as ab45937)

  • General notes

    Previously labelled as NTF97/Importin beta. 

Properties

  • Form

    Liquid
  • Storage instructions

    Shipped at 4°C. Store at +4°C short term (1-2 weeks). Upon delivery aliquot. Store at -20°C or -80°C. Avoid freeze / thaw cycle.
  • Storage buffer

    pH: 7.40
    Preservative: 0.02% Sodium azide
    Constituent: PBS

    Batches of this product that have a concentration < 1mg/ml may have BSA added as a stabilising agent. If you would like information about the formulation of a specific lot, please contact our scientific support team who will be happy to help.
  • Concentration information loading...
  • Purity

    Immunogen affinity purified
  • Clonality

    Polyclonal
  • Isotype

    IgG
  • Research areas

    • Tags & Cell Markers
    • Subcellular Markers
    • Nucleus
    • Nuclear Envelope
    • Signal Transduction
    • Protein Trafficking
    • Nuclear Import / Export
    • Epigenetics and Nuclear Signaling
    • Nuclear Signaling Pathways
    • Nuclear Receptors
    • Nuclear Pore Complex

Associated products

  • Compatible Secondaries

    • Goat Anti-Rabbit IgG H&L (Alexa Fluor® 488) (ab150077)
    • Goat Anti-Rabbit IgG H&L (HRP) (ab205718)
  • Isotype control

    • Rabbit IgG, polyclonal - Isotype Control (ChIP Grade) (ab171870)

Applications

The Abpromise guarantee

Our Abpromise guarantee covers the use of ab45938 in the following tested applications.

The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.

Guaranteed

Tested applications are guaranteed to work and covered by our Abpromise guarantee.

Predicted

Predicted to work for this combination of applications and species but not guaranteed.

Incompatible

Does not work for this combination of applications and species.

Application Species
IHC-P
Human
IP
Human
WB
Human
Application Abreviews Notes
IP
Use a concentration of 5 µg/ml.
IHC-P
Use a concentration of 1 µg/ml. Perform heat mediated antigen retrieval before commencing with IHC staining protocol.
WB
Use a concentration of 2 µg/ml. Detects a band of approximately 97 kDa (predicted molecular weight: 97 kDa).
Notes
IP
Use a concentration of 5 µg/ml.
IHC-P
Use a concentration of 1 µg/ml. Perform heat mediated antigen retrieval before commencing with IHC staining protocol.
WB
Use a concentration of 2 µg/ml. Detects a band of approximately 97 kDa (predicted molecular weight: 97 kDa).

Target

  • Function

    Functions in nuclear protein import, either in association with an adapter protein, like an importin-alpha subunit, which binds to nuclear localization signals (NLS) in cargo substrates, or by acting as autonomous nuclear transport receptor. Acting autonomously, serves itself as NLS receptor. Docking of the importin/substrate complex to the nuclear pore complex (NPC) is mediated by KPNB1 through binding to nucleoporin FxFG repeats and the complex is subsequently translocated through the pore by an energy requiring, Ran-dependent mechanism. At the nucleoplasmic side of the NPC, Ran binds to importin-beta and the three components separate and importin-alpha and -beta are re-exported from the nucleus to the cytoplasm where GTP hydrolysis releases Ran from importin. The directionality of nuclear import is thought to be conferred by an asymmetric distribution of the GTP- and GDP-bound forms of Ran between the cytoplasm and nucleus. Mediates autonomously the nuclear import of ribosomal proteins RPL23A, RPS7 and RPL5. Binds to a beta-like import receptor binding (BIB) domain of RPL23A. In association with IPO7 mediates the nuclear import of H1 histone. In vitro, mediates nuclear import of H2A, H2B, H3 and H4 histones. In case of HIV-1 infection, binds and mediates the nuclear import of HIV-1 Rev. Imports PRKCI into the nucleus.
  • Sequence similarities

    Belongs to the importin beta family.
    Contains 8 HEAT repeats.
    Contains 1 importin N-terminal domain.
  • Cellular localization

    Cytoplasm. Nucleus envelope.
  • Target information above from: UniProt accession Q14974 The UniProt Consortium
    The Universal Protein Resource (UniProt) in 2010
    Nucleic Acids Res. 38:D142-D148 (2010) .

    Information by UniProt
  • Database links

    • Entrez Gene: 3837 Human
    • Entrez Gene: 16211 Mouse
    • Entrez Gene: 24917 Rat
    • Omim: 602738 Human
    • SwissProt: Q14974 Human
    • SwissProt: P70168 Mouse
    • SwissProt: P52296 Rat
    • Unigene: 532793 Human
    • Unigene: 706168 Human
    • Unigene: 251013 Mouse
    • Unigene: 11061 Rat
    see all
  • Alternative names

    • IMB 1 antibody
    • IMB1 antibody
    • IMB1_HUMAN antibody
    • Impnb antibody
    • Importin 90 antibody
    • Importin beta 1 antibody
    • Importin beta 1 subunit antibody
    • Importin subunit beta-1 antibody
    • Importin-90 antibody
    • IPOB antibody
    • Karyopherin beta 1 antibody
    • Karyopherin beta 1 subunit antibody
    • Karyopherin subunit beta-1 antibody
    • KPNB 1 antibody
    • Kpnb1 antibody
    • MGC2155 antibody
    • MGC2156 antibody
    • MGC2157 antibody
    • NTF 97 antibody
    • NTF97 antibody
    • NTF97/Importin beta antibody
    • Nuclear factor P97 antibody
    • Pore targeting complex 97 kDa subunit antibody
    • PTAC97 antibody
    see all

Images

  • Western blot - Anti-KPNB1 antibody (ab45938)
    Western blot - Anti-KPNB1 antibody (ab45938)
    All lanes : Anti-KPNB1 antibody (ab45938) at 2 µg/ml

    Lane 1 : HeLa (Human epithelial carcinoma cell line) Whole Cell Lysate
    Lane 2 : Jurkat (Human T cell lymphoblast-like cell line) Whole Cell Lysate
    Lane 3 : NIH/3T3 whole cell lysate (ab7179)
    Lane 4 : PC12 (Rat adrenal pheochromocytoma cell line) Whole Cell Lysate

    Lysates/proteins at 15 µg per lane.

    Secondary
    All lanes : Goat polyclonal to Rabbit IgG - H&L - Pre-Adsorbed (HRP) at 1/3000 dilution

    Performed under reducing conditions.

    Predicted band size: 97 kDa
    Observed band size: 97 kDa

  • Immunoprecipitation - Anti-KPNB1 antibody (ab45938)
    Immunoprecipitation - Anti-KPNB1 antibody (ab45938)

    KPNB1 was immunoprecipitated using 0.5mg Hela whole cell extract, 5µg of Rabbit polyclonal to KPNB1 and 50µl of protein G magnetic beads (+). No antibody was added to the control (-).
    The antibody was incubated under agitation with Protein G beads for 10min, Hela whole cell extract lysate diluted in RIPA buffer was added to each sample and incubated for a further 10min under agitation.
    Proteins were eluted by addition of 40µl SDS loading buffer and incubated for 10min at 70oC; 10µl of each sample was separated on a SDS PAGE gel, transferred to a nitrocellulose membrane, blocked with 5% BSA and probed with ab45938.
    Secondary: Mouse monoclonal [SB62a] Secondary Antibody to Rabbit IgG light chain (HRP) (ab99697).
    Band: 97kDa; KPNB1 

  • Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-KPNB1 antibody (ab45938)
    Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-KPNB1 antibody (ab45938)

    IHC image of KPNB1 staining in a formalin fixed, paraffin embedded normal human testis tissue section*, performed on a Leica Bond™ system using the standard protocol F. The section was pre-treated using heat mediated antigen retrieval with sodium citrate buffer (pH6, epitope retrieval solution 1) for 20 mins. The section was then incubated with ab45938, 5µg/ml, for 15 mins at room temperature and detected using an HRP conjugated compact polymer system. DAB was used as the chromogen. The section was then counterstained with haematoxylin and mounted with DPX.

     

    For other IHC staining systems (automated and non-automated) customers should optimize variable parameters such as antigen retrieval conditions, primary antibody concentration and antibody incubation times.

    *Tissue obtained from the Human Research Tissue Bank, supported by the NIHR Cambridge Biomedical Research Centre

Protocols

  • Immunoprecipitation protocols
  • Immunohistochemistry protocols
  • Western blot protocols

Click here to view the general protocols

Datasheets and documents

    • Datasheet
  • References (7)

    Publishing research using ab45938? Please let us know so that we can cite the reference in this datasheet.

    ab45938 has been referenced in 7 publications.

    • Ivic N  et al. Fuzzy Interactions Form and Shape the Histone Transport Complex. Mol Cell 73:1191-1203.e6 (2019). PubMed: 30824373
    • Guo J  et al. The transmembrane nucleoporin Pom121 ensures efficient HIV-1 pre-integration complex nuclear import. Virology 521:169-174 (2018). Human . PubMed: 29957337
    • Chen WS  et al. Ran-dependent TPX2 activation promotes acentrosomal microtubule nucleation in neurons. Sci Rep 7:42297 (2017). PubMed: 28205572
    • Jing R  et al. Motifs in the amino-terminus of CENP-A are required for its accumulation within the nucleus and at the centromere. Oncotarget 8:40654-40667 (2017). PubMed: 28489565
    • Zhu Y  et al. Subcellular Distribution of HDAC1 in Neurotoxic Conditions Is Dependent on Serine Phosphorylation. J Neurosci 37:7547-7559 (2017). PubMed: 28663197
    • Wiezlak M  et al. G-actin regulates the shuttling and PP1 binding of the RPEL protein Phactr1 to control actomyosin assembly. J Cell Sci 125:5860-72 (2012). PubMed: 22976292
    • Talati M  et al. Altered expression of nuclear and cytoplasmic histone H1 in pulmonary artery and pulmonary artery smooth muscle cells in patients with IPAH. Pulm Circ 2:340-51 (2012). PubMed: 23130102

    Customer reviews and Q&As

    Show All Reviews Q&A
    Submit a review Submit a question

    1-2 of 2 Abreviews or Q&A

    Question

    I'm looking for an antibody that will cross react with fly Importin beta (Ketel). There are currently no commercially available antibodies for fly Importin beta. You have several Anti-NTF97/Importin beta antibodies available. Do you know if any of them cross react with drosophila Importin beta?
    If it is unknown if they cross react with fly, would it be possible for you to send small aliquots of your anti-NTF97/Importin beta antibodies so that I may test them. If one or more do indeed cross react, I can send you files of corresponding Western Blot analysis.

    Anti-NTF97/Importin beta antibodies:
    ab36775
    ab45901
    ab45938
    ab50417
    ab115882
    ab2811

    Read More

    Abcam community

    Verified customer

    Asked on Jan 19 2012

    Answer

    Thank you for contacting us.


    I have run alignments of the immunogens used to create each of these products against drosophila Importin beta. Unfortunately, each immunogen had less than 70% homology with your target and most were less than 50%. We cannot recommend a product or offera product for testingif the matched identity of the immunogen is less than 85%.

    I hope this information is helpful and wish you luck in finding a Importin beta antibody to suit your needs.Please do not hesitate to contact us if you need any more advice or information.

    Read More

    Abcam Scientific Support

    Answered on Jan 19 2012

    Immunocytochemistry/ Immunofluorescence abreview for Anti-KPNB1 antibody

    Good
    Abreviews
    Abreviews
    abreview image
    Application
    Immunocytochemistry/ Immunofluorescence
    Sample
    Human Cell (HeLa)
    Permeabilization
    Yes - 0.5% Triton X100 in PBS
    Specification
    HeLa
    Fixative
    Paraformaldehyde
    Read More

    Dr. Kirk Mcmanus

    Verified customer

    Submitted Feb 19 2008

    Please note: All products are "FOR RESEARCH USE ONLY. NOT FOR USE IN DIAGNOSTIC PROCEDURES"
    For licensing inquiries, please contact partnerships@abcam.com

    Get resources and offers direct to your inbox Sign up
    A-Z by research area
    • Cancer
    • Cardiovascular
    • Cell biology
    • Developmental biology
    • Epigenetics & Nuclear signaling
    • Immunology
    • Metabolism
    • Microbiology
    • Neuroscience
    • Signal transduction
    • Stem cells
    A-Z by product type
    • Primary antibodies
    • Secondary antibodies
    • Biochemicals
    • Isotype controls
    • Flow cytometry multi-color selector
    • Kits
    • Loading controls
    • Lysates
    • Peptides
    • Proteins
    • Slides
    • Tags and cell markers
    • Tools & Reagents
    Help & support
    • Support
    • Make an Inquiry
    • Protocols & troubleshooting
    • Placing an order
    • RabMAb products
    • Biochemical product FAQs
    • Training
    • Browse by Target
    Company
    • Corporate site
    • Investor relations
    • Company news
    • Careers
    • About us
    • Blog
    Events
    • Tradeshows
    • Conferences
    International websites
    • abcam.cn
    • abcam.co.jp

    Join with us

    • LinkedIn
    • facebook
    • Twitter
    • YouTube
    • Terms of sale
    • Website terms of use
    • Cookie policy
    • Privacy policy
    • Legal
    • Modern slavery statement
    © 1998-2021 Abcam plc. All rights reserved.