Immunocytochemistry/ Immunofluorescence abreview for Anti-KPNB1 antibody

Good
Abreviews
Application
Immunocytochemistry/ Immunofluorescence
Sample
Human Cell (HeLa)
Permeabilization
Yes - 0.5% Triton X100 in PBS
Specification
HeLa
Fixative
Paraformaldehyde

Other product details

Incubation time
1 hour(s) and 0 minute(s) · Temperature: 22°C · Diluent: PBS
Dilution
1/200

Secondary antibody

Dilution
1/200
Name
Non-Abcam antibody was used: Goat anti-Rabbit Cy3
Host species: Goat
Clonality: Polyclonal
Conjugation: Cy3 ®

Additional data

Additional Notes
Asynchronouse HeLa cells were paraformaldehyde fixed (4%, 10min), permeablized and immunofluorescently labeled at 1:200 for 1hr. A predominantly focal nuclear staining pattern is observed.

Dr. Kirk Mcmanus

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Submitted Feb 19 2008

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