Anti-KPNB1 antibody [OTI6H3] (ab236508)
Key features and details
- Mouse monoclonal [OTI6H3] to KPNB1
- Suitable for: WB, IHC-P
- Reacts with: Mouse, Rat, Human
- Isotype: IgG1
Overview
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Product name
Anti-KPNB1 antibody [OTI6H3]
See all KPNB1 primary antibodies -
Description
Mouse monoclonal [OTI6H3] to KPNB1 -
Host species
Mouse -
Tested applications
Suitable for: WB, IHC-Pmore details -
Species reactivity
Reacts with: Mouse, Rat, Human -
Immunogen
Recombinant fragment corresponding to Human KPNB1 aa 1-200. (NP_002256) produced in E.coli.
Database link: Q14974 -
Positive control
- WB: HEK-293T transfected with pCMV6-ENTRY KPNB1; HeLa, A431, NIH/3T3 and PC-12 whole cell lysates. IHC-P: Human adult heart tissue.
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General notes
The Life Science industry has been in the grips of a reproducibility crisis for a number of years. Abcam is leading the way in addressing this with our range of recombinant monoclonal antibodies and knockout edited cell lines for gold-standard validation. Please check that this product meets your needs before purchasing.
If you have any questions, special requirements or concerns, please send us an inquiry and/or contact our Support team ahead of purchase. Recommended alternatives for this product can be found below, along with publications, customer reviews and Q&As
Properties
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Form
Liquid -
Storage instructions
Shipped at 4°C. Store at +4°C short term (1-2 weeks). Upon delivery aliquot. Store at -20°C long term. Avoid freeze / thaw cycle. -
Storage buffer
pH: 7.30
Preservative: 0.02% Sodium azide
Constituents: PBS, 1% BSA, 50% Glycerol -
Concentration information loading...
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Purity
Affinity purified -
Purification notes
Purified from cell culture supernatant. -
Clonality
Monoclonal -
Clone number
OTI6H3 -
Isotype
IgG1 -
Research areas
Associated products
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Compatible Secondaries
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Isotype control
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Positive Controls
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Related Products
Applications
The Abpromise guarantee
Our Abpromise guarantee covers the use of ab236508 in the following tested applications.
The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.
Application | Abreviews | Notes |
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WB |
1/500 - 1/2000. Predicted molecular weight: 97 kDa.
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IHC-P |
1/2000.
Heat-induced epitope retrieval by 1 mM EDTA in 10 mM Tris buffer (pH 8.5) at 120°C for 3 minutes |
Notes |
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WB
1/500 - 1/2000. Predicted molecular weight: 97 kDa. |
IHC-P
1/2000. Heat-induced epitope retrieval by 1 mM EDTA in 10 mM Tris buffer (pH 8.5) at 120°C for 3 minutes |
Target
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Function
Functions in nuclear protein import, either in association with an adapter protein, like an importin-alpha subunit, which binds to nuclear localization signals (NLS) in cargo substrates, or by acting as autonomous nuclear transport receptor. Acting autonomously, serves itself as NLS receptor. Docking of the importin/substrate complex to the nuclear pore complex (NPC) is mediated by KPNB1 through binding to nucleoporin FxFG repeats and the complex is subsequently translocated through the pore by an energy requiring, Ran-dependent mechanism. At the nucleoplasmic side of the NPC, Ran binds to importin-beta and the three components separate and importin-alpha and -beta are re-exported from the nucleus to the cytoplasm where GTP hydrolysis releases Ran from importin. The directionality of nuclear import is thought to be conferred by an asymmetric distribution of the GTP- and GDP-bound forms of Ran between the cytoplasm and nucleus. Mediates autonomously the nuclear import of ribosomal proteins RPL23A, RPS7 and RPL5. Binds to a beta-like import receptor binding (BIB) domain of RPL23A. In association with IPO7 mediates the nuclear import of H1 histone. In vitro, mediates nuclear import of H2A, H2B, H3 and H4 histones. In case of HIV-1 infection, binds and mediates the nuclear import of HIV-1 Rev. Imports PRKCI into the nucleus. -
Sequence similarities
Belongs to the importin beta family.
Contains 8 HEAT repeats.
Contains 1 importin N-terminal domain. -
Cellular localization
Cytoplasm. Nucleus envelope. - Information by UniProt
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Database links
- Entrez Gene: 3837 Human
- Entrez Gene: 16211 Mouse
- Entrez Gene: 24917 Rat
- Omim: 602738 Human
- SwissProt: Q14974 Human
- SwissProt: P70168 Mouse
- SwissProt: P52296 Rat
- Unigene: 532793 Human
see all -
Alternative names
- IMB 1 antibody
- IMB1 antibody
- IMB1_HUMAN antibody
see all
Images
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All lanes : Anti-KPNB1 antibody [OTI6H3] (ab236508) at 1/2000 dilution
Lane 1 : HEK-293T (human epithelial cell line from embryonic kidney transformed with large T antigen) transfected with pCMV6-ENTRY control whole cell lysate
Lane 2 : HEK-293T (human epithelial cell line from embryonic kidney transformed with large T antigen) transfected with pCMV6-ENTRY KPNB1
Lysates/proteins at 5 µg per lane.
Predicted band size: 97 kDa -
All lanes : Anti-KPNB1 antibody [OTI6H3] (ab236508) at 1/500 dilution
Lane 1 : HeLa (human epithelial cell line from cervix adenocarcinoma) whole cell extract
Lane 2 : A431 (human epidermoid carcinoma cell line) whole cell extract
Lane 3 : NIH/3T3 (mouse embryo fibroblast cell line) whole cell extract
Lane 4 : PC-12 (rat adrenal gland pheochromocytoma cell line) whole cell extract
Lysates/proteins at 35 µg per lane.
Predicted band size: 97 kDa -
Paraffin-embedded human adult heart tissue stained for KPNB1 using ab236508 at 1/2000 dilution in immunohistochemical analysis.
Protocols
To our knowledge, customised protocols are not required for this product. Please try the standard protocols listed below and let us know how you get on.
Datasheets and documents
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SDS download
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Datasheet download
References (0)
ab236508 has not yet been referenced specifically in any publications.