Key features and details
- Mouse monoclonal [OTI6H3] to KPNB1
- Suitable for: WB, IHC-P
- Reacts with: Mouse, Rat, Human
- Isotype: IgG1
Product nameAnti-KPNB1 antibody [OTI6H3]
See all KPNB1 primary antibodies
DescriptionMouse monoclonal [OTI6H3] to KPNB1
Tested applicationsSuitable for: WB, IHC-Pmore details
Species reactivityReacts with: Mouse, Rat, Human
Recombinant fragment corresponding to Human KPNB1 aa 1-200. (NP_002256) produced in E.coli.
Database link: Q14974
- WB: HEK-293T transfected with pCMV6-ENTRY KPNB1; HeLa, A431, NIH/3T3 and PC-12 whole cell lysates. IHC-P: Human adult heart tissue.
Previously labelled as NTF97/Importin beta.
Storage instructionsShipped at 4°C. Store at +4°C short term (1-2 weeks). Upon delivery aliquot. Store at -20°C long term. Avoid freeze / thaw cycle.
Storage bufferpH: 7.30
Preservative: 0.02% Sodium azide
Constituents: PBS, 1% BSA, 50% Glycerol
Concentration information loading...
Purification notesPurified from cell culture supernatant.
Our Abpromise guarantee covers the use of ab236508 in the following tested applications.
The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.
|WB||1/500 - 1/2000. Predicted molecular weight: 97 kDa.|
Heat-induced epitope retrieval by 1 mM EDTA in 10 mM Tris buffer (pH 8.5) at 120°C for 3 minutes
FunctionFunctions in nuclear protein import, either in association with an adapter protein, like an importin-alpha subunit, which binds to nuclear localization signals (NLS) in cargo substrates, or by acting as autonomous nuclear transport receptor. Acting autonomously, serves itself as NLS receptor. Docking of the importin/substrate complex to the nuclear pore complex (NPC) is mediated by KPNB1 through binding to nucleoporin FxFG repeats and the complex is subsequently translocated through the pore by an energy requiring, Ran-dependent mechanism. At the nucleoplasmic side of the NPC, Ran binds to importin-beta and the three components separate and importin-alpha and -beta are re-exported from the nucleus to the cytoplasm where GTP hydrolysis releases Ran from importin. The directionality of nuclear import is thought to be conferred by an asymmetric distribution of the GTP- and GDP-bound forms of Ran between the cytoplasm and nucleus. Mediates autonomously the nuclear import of ribosomal proteins RPL23A, RPS7 and RPL5. Binds to a beta-like import receptor binding (BIB) domain of RPL23A. In association with IPO7 mediates the nuclear import of H1 histone. In vitro, mediates nuclear import of H2A, H2B, H3 and H4 histones. In case of HIV-1 infection, binds and mediates the nuclear import of HIV-1 Rev. Imports PRKCI into the nucleus.
Sequence similaritiesBelongs to the importin beta family.
Contains 8 HEAT repeats.
Contains 1 importin N-terminal domain.
Cellular localizationCytoplasm. Nucleus envelope.
- Information by UniProt
- IMB 1 antibody
- IMB1 antibody
- IMB1_HUMAN antibody
All lanes : Anti-KPNB1 antibody [OTI6H3] (ab236508) at 1/2000 dilution
Lane 1 : HEK-293T (human epithelial cell line from embryonic kidney transformed with large T antigen) transfected with pCMV6-ENTRY control whole cell lysate
Lane 2 : HEK-293T (human epithelial cell line from embryonic kidney transformed with large T antigen) transfected with pCMV6-ENTRY KPNB1
Lysates/proteins at 5 µg per lane.
Predicted band size: 97 kDa
All lanes : Anti-KPNB1 antibody [OTI6H3] (ab236508) at 1/500 dilution
Lane 1 : HeLa (human epithelial cell line from cervix adenocarcinoma) whole cell extract
Lane 2 : A431 (human epidermoid carcinoma cell line) whole cell extract
Lane 3 : NIH/3T3 (mouse embryo fibroblast cell line) whole cell extract
Lane 4 : PC-12 (rat adrenal gland pheochromocytoma cell line) whole cell extract
Lysates/proteins at 35 µg per lane.
Predicted band size: 97 kDa
Paraffin-embedded human adult heart tissue stained for KPNB1 using ab236508 at 1/2000 dilution in immunohistochemical analysis.
To our knowledge, customised protocols are not required for this product. Please try the standard protocols listed below and let us know how you get on.
ab236508 has not yet been referenced specifically in any publications.