Overview

  • Product name

    Anti-KPNB1 antibody [OTI6H3]
    See all KPNB1 primary antibodies
  • Description

    Mouse monoclonal [OTI6H3] to KPNB1
  • Host species

    Mouse
  • Tested applications

    Suitable for: WB, IHC-Pmore details
  • Species reactivity

    Reacts with: Mouse, Rat, Human
  • Immunogen

    Recombinant fragment corresponding to Human KPNB1 aa 1-200. (NP_002256) produced in E.coli.
    Database link: Q14974

  • Positive control

    • WB: HEK-293T transfected with pCMV6-ENTRY KPNB1; HeLa, A431, NIH/3T3 and PC-12 whole cell lysates. IHC-P: Human adult heart tissue.
  • General notes

    Previously labelled as NTF97/Importin beta. 

Properties

Applications

Our Abpromise guarantee covers the use of ab236508 in the following tested applications.

The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.

Application Abreviews Notes
WB 1/500 - 1/2000. Predicted molecular weight: 97 kDa.
IHC-P 1/2000.

Heat-induced epitope retrieval by 1 mM EDTA in 10 mM Tris buffer (pH 8.5) at 120°C for 3 minutes

Target

  • Function

    Functions in nuclear protein import, either in association with an adapter protein, like an importin-alpha subunit, which binds to nuclear localization signals (NLS) in cargo substrates, or by acting as autonomous nuclear transport receptor. Acting autonomously, serves itself as NLS receptor. Docking of the importin/substrate complex to the nuclear pore complex (NPC) is mediated by KPNB1 through binding to nucleoporin FxFG repeats and the complex is subsequently translocated through the pore by an energy requiring, Ran-dependent mechanism. At the nucleoplasmic side of the NPC, Ran binds to importin-beta and the three components separate and importin-alpha and -beta are re-exported from the nucleus to the cytoplasm where GTP hydrolysis releases Ran from importin. The directionality of nuclear import is thought to be conferred by an asymmetric distribution of the GTP- and GDP-bound forms of Ran between the cytoplasm and nucleus. Mediates autonomously the nuclear import of ribosomal proteins RPL23A, RPS7 and RPL5. Binds to a beta-like import receptor binding (BIB) domain of RPL23A. In association with IPO7 mediates the nuclear import of H1 histone. In vitro, mediates nuclear import of H2A, H2B, H3 and H4 histones. In case of HIV-1 infection, binds and mediates the nuclear import of HIV-1 Rev. Imports PRKCI into the nucleus.
  • Sequence similarities

    Belongs to the importin beta family.
    Contains 8 HEAT repeats.
    Contains 1 importin N-terminal domain.
  • Cellular localization

    Cytoplasm. Nucleus envelope.
  • Information by UniProt
  • Database links

  • Alternative names

    • IMB 1 antibody
    • IMB1 antibody
    • IMB1_HUMAN antibody
    • Impnb antibody
    • Importin 90 antibody
    • Importin beta 1 antibody
    • Importin beta 1 subunit antibody
    • Importin subunit beta-1 antibody
    • Importin-90 antibody
    • IPOB antibody
    • Karyopherin beta 1 antibody
    • Karyopherin beta 1 subunit antibody
    • Karyopherin subunit beta-1 antibody
    • KPNB 1 antibody
    • Kpnb1 antibody
    • MGC2155 antibody
    • MGC2156 antibody
    • MGC2157 antibody
    • NTF 97 antibody
    • NTF97 antibody
    • NTF97/Importin beta antibody
    • Nuclear factor P97 antibody
    • Pore targeting complex 97 kDa subunit antibody
    • PTAC97 antibody
    see all

Images

  • All lanes : Anti-KPNB1 antibody [OTI6H3] (ab236508) at 1/2000 dilution

    Lane 1 : HEK-293T (human epithelial cell line from embryonic kidney transformed with large T antigen) transfected with pCMV6-ENTRY control whole cell lysate
    Lane 2 : HEK-293T (human epithelial cell line from embryonic kidney transformed with large T antigen) transfected with pCMV6-ENTRY KPNB1

    Lysates/proteins at 5 µg per lane.

    Predicted band size: 97 kDa

  • All lanes : Anti-KPNB1 antibody [OTI6H3] (ab236508) at 1/500 dilution

    Lane 1 : HeLa (human epithelial cell line from cervix adenocarcinoma) whole cell extract
    Lane 2 : A431 (human epidermoid carcinoma cell line) whole cell extract
    Lane 3 : NIH/3T3 (mouse embryo fibroblast cell line) whole cell extract
    Lane 4 : PC-12 (rat adrenal gland pheochromocytoma cell line) whole cell extract

    Lysates/proteins at 35 µg per lane.

    Predicted band size: 97 kDa

  • Paraffin-embedded human adult heart tissue stained for KPNB1 using ab236508 at 1/2000 dilution in immunohistochemical analysis.

References

ab236508 has not yet been referenced specifically in any publications.

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