Overview

  • Product name
    Anti-KPRP antibody
  • Description
    Rabbit polyclonal to KPRP
  • Host species
    Rabbit
  • Tested applications
    Suitable for: IHC-P, ICC/IFmore details
  • Species reactivity
    Reacts with: Human
  • Immunogen

    antigen sequence: PNPVPYPGDL GCHESSPHRL DTEAPYCGPS SYNQGQESGA GCGPGDVFPE RRGQDGHGDQ GNAFAGVKGE AK, corresponding to amino acids of Human KPRP.

    Run BLAST with BLAST the sequence with ExPASy Run BLAST with BLAST the sequence with NCBI
  • Positive control
    • Human skin tissue.

Properties

  • Form
    Liquid
  • Storage instructions
    Shipped at 4°C. Store at -20°C or -80°C. Avoid freeze / thaw cycle. Store undiluted.
  • Storage buffer
    pH: 7.20
    Preservative: 0.02% Sodium azide
    Constituents: 59% PBS, 40% Glycerol
  • Concentration information loading...
  • Purity
    Immunogen affinity purified
  • Clonality
    Polyclonal
  • Isotype
    IgG
  • Research areas

Applications

Our Abpromise guarantee covers the use of ab122201 in the following tested applications.

The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.

Application Abreviews Notes
IHC-P 1/200 - 1/500. Perform heat mediated antigen retrieval with citrate buffer pH 6 before commencing with IHC staining protocol.
ICC/IF Use a concentration of 1 - 4 µg/ml.

Recommend PFA Fixation and Triton X-100 treatment

Target

  • Tissue specificity
    Expressed in the upper layer of epidermis and psoriasis (at protein level). Expressed in the upper layer of epidermis and psoriasis.
  • Developmental stage
    Expressed in the periderm of fetal skin from 16th week gestational age and in the granular and horny layers of the epidermis at 24th week gestational age and thereafter (at protein level).
  • Cellular localization
    Cytoplasm.
  • Information by UniProt
  • Database links
    • Alternative names
      • C1orf45 antibody
      • hKPRP antibody
      • Keratinocyte expressed, proline-rich protein antibody
      • Keratinocyte proline-rich protein antibody
      • Kprp antibody
      • KPRP_HUMAN antibody
      see all

    Images

    • Immunocytochemistry/ Immunofluorescence - Anti-KPRP antibody (ab122201)
      Immunocytochemistry/ Immunofluorescence - Anti-KPRP antibody (ab122201)
      Immunofluorescent staining of Human cell line U-251MG shows positivity in plasma membrane and cytoplasm. Recommended concentration of ab122201 1-4 µg/ml. Cells treated with PFA/Triton X-100.
    • Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-KPRP antibody (ab122201)
      Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-KPRP antibody (ab122201)
      ab122201, at 1/200 dilution, staining KPRP in paraffin-embedded Human skin tissue by Immunohistochemistry.

    References

    ab122201 has not yet been referenced specifically in any publications.

    Publishing research using ab122201? Please let us know so that we can cite the reference in this datasheet.

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