Anti-KPRP antibody (ab122201)

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Key features and details

  • Rabbit polyclonal to KPRP
  • Suitable for: IHC-P, ICC/IF
  • Reacts with: Human
  • Isotype: IgG

Overview

  • Product name

    Anti-KPRP antibody

  • Description

    Rabbit polyclonal to KPRP

  • Host species

    Rabbit

  • Tested applications

    Suitable for: IHC-P, ICC/IFmore details

  • Species reactivity

    Reacts with: Human

  • Immunogen

    antigen sequence: PNPVPYPGDL GCHESSPHRL DTEAPYCGPS SYNQGQESGA GCGPGDVFPE RRGQDGHGDQ GNAFAGVKGE AK, corresponding to amino acids of Human KPRP.

    Run BLAST with BLAST the sequence with ExPASy Run BLAST with BLAST the sequence with NCBI

  • Positive control

    • Human skin tissue.

  • General notes

    Reproducibility is key to advancing scientific discovery and accelerating scientists’ next breakthrough.

    Abcam is leading the way with our range of recombinant antibodies, knockout-validated antibodies and knockout cell lines, all of which support improved reproducibility.

    We are also planning to innovate the way in which we present recommended applications and species on our product datasheets, so that only applications & species that have been tested in our own labs, our suppliers or by selected trusted collaborators are covered by our Abpromise™ guarantee.

    In preparation for this, we have started to update the applications & species that this product is Abpromise guaranteed for.

    We are also updating the applications & species that this product has been “predicted to work with,” however this information is not covered by our Abpromise guarantee.

    Applications & species from publications and Abreviews that have not been tested in our own labs or in those of our suppliers are not covered by the Abpromise guarantee.

    Please check that this product meets your needs before purchasing. If you have any questions, special requirements or concerns, please send us an inquiry and/or contact our Support team ahead of purchase. Recommended alternatives for this product can be found below, as well as customer reviews and Q&As.

Properties

  • Form

    Liquid

  • Storage instructions

    Shipped at 4°C. Store at -20°C or -80°C. Avoid freeze / thaw cycle. Store undiluted.

  • Storage buffer

    pH: 7.20
    Preservative: 0.02% Sodium azide
    Constituents: 40% Glycerol (glycerin, glycerine), 59% PBS
  • Concentration information loading...

  • Purity

    Immunogen affinity purified

  • Clonality

    Polyclonal

  • Isotype

    IgG

  • Research areas

Applications

Our Abpromise guarantee covers the use of ab122201 in the following tested applications.

The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.

Application Abreviews Notes
IHC-P 1/200 - 1/500. Perform heat mediated antigen retrieval with citrate buffer pH 6 before commencing with IHC staining protocol.
ICC/IF Use a concentration of 1 - 4 µg/ml.

Recommend PFA Fixation and Triton X-100 treatment

Target

  • Tissue specificity

    Expressed in the upper layer of epidermis and psoriasis (at protein level). Expressed in the upper layer of epidermis and psoriasis.

  • Developmental stage

    Expressed in the periderm of fetal skin from 16th week gestational age and in the granular and horny layers of the epidermis at 24th week gestational age and thereafter (at protein level).

  • Cellular localization

    Cytoplasm.
  • Information by UniProt

  • Database links

    • Alternative names

      • C1orf45 antibody
      • hKPRP antibody
      • Keratinocyte expressed, proline-rich protein antibody
      • Keratinocyte proline-rich protein antibody
      • Kprp antibody
      • KPRP_HUMAN antibody
      see all

    Images

    • Immunocytochemistry/ Immunofluorescence - Anti-KPRP antibody (ab122201)
      Immunocytochemistry/ Immunofluorescence - Anti-KPRP antibody (ab122201)
      Immunofluorescent staining of Human cell line U-251MG shows positivity in plasma membrane and cytoplasm. Recommended concentration of ab122201 1-4 µg/ml. Cells treated with PFA/Triton X-100.
    • Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-KPRP antibody (ab122201)
      Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-KPRP antibody (ab122201)
      ab122201, at 1/200 dilution, staining KPRP in paraffin-embedded Human skin tissue by Immunohistochemistry.

    References (1)

    Publishing research using ab122201? Please let us know so that we can cite the reference in this datasheet.

    ab122201 has been referenced in 1 publication.

    • Suga H  et al. Keratinocyte Proline-Rich Protein Deficiency in Atopic Dermatitis Leads to Barrier Disruption. J Invest Dermatol 139:1867-1875.e7 (2019). PubMed: 30905808

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