Anti-KPRP antibody (ab122201)
- Datasheet
- References
- Protocols
Overview
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Product name
Anti-KPRP antibody -
Description
Rabbit polyclonal to KPRP -
Host species
Rabbit -
Tested applications
Suitable for: IHC-P, ICC/IFmore details -
Species reactivity
Reacts with: Human -
Immunogen
antigen sequence: PNPVPYPGDL GCHESSPHRL DTEAPYCGPS SYNQGQESGA GCGPGDVFPE RRGQDGHGDQ GNAFAGVKGE AK, corresponding to amino acids of Human KPRP.
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Positive control
- Human skin tissue.
Properties
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Form
Liquid -
Storage instructions
Shipped at 4°C. Store at -20°C or -80°C. Avoid freeze / thaw cycle. Store undiluted. -
Storage buffer
pH: 7.20
Preservative: 0.02% Sodium azide
Constituents: 59% PBS, 40% Glycerol -
Concentration information loading...
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Purity
Immunogen affinity purified -
Clonality
Polyclonal -
Isotype
IgG -
Research areas
Associated products
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Compatible Secondaries
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Isotype control
Applications
Our Abpromise guarantee covers the use of ab122201 in the following tested applications.
The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.
Application | Abreviews | Notes |
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IHC-P | 1/200 - 1/500. Perform heat mediated antigen retrieval with citrate buffer pH 6 before commencing with IHC staining protocol. | |
ICC/IF | Use a concentration of 1 - 4 µg/ml. Recommend PFA Fixation and Triton X-100 treatment |
Target
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Tissue specificity
Expressed in the upper layer of epidermis and psoriasis (at protein level). Expressed in the upper layer of epidermis and psoriasis. -
Developmental stage
Expressed in the periderm of fetal skin from 16th week gestational age and in the granular and horny layers of the epidermis at 24th week gestational age and thereafter (at protein level). -
Cellular localization
Cytoplasm. - Information by UniProt
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Database links
- Entrez Gene: 448834 Human
- Omim: 613260 Human
- SwissProt: Q5T749 Human
- Unigene: 149386 Human
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Alternative names
- C1orf45 antibody
- hKPRP antibody
- Keratinocyte expressed, proline-rich protein antibody
see all
Images
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Immunofluorescent staining of Human cell line U-251MG shows positivity in plasma membrane and cytoplasm. Recommended concentration of ab122201 1-4 µg/ml. Cells treated with PFA/Triton X-100.
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Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-KPRP antibody (ab122201)ab122201, at 1/200 dilution, staining KPRP in paraffin-embedded Human skin tissue by Immunohistochemistry.
Datasheets and documents
References
ab122201 has not yet been referenced specifically in any publications.