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Signal Transduction Signaling Pathway G Protein Signaling Small G Proteins Ras Family
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RecombinantRabMAb

Recombinant Anti-KRAS antibody [EPR23474-76] (ab275876)

  • Datasheet
  • Certificate of Compliance
Reviews (8) Submit a question References (1)

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Western blot - Anti-KRAS antibody [EPR23474-76] (ab275876)
  • Flow Cytometry (Intracellular) - Anti-KRAS antibody [EPR23474-76] (ab275876)
  • Immunoprecipitation - Anti-KRAS antibody [EPR23474-76] (ab275876)
  • Western blot - Anti-KRAS antibody [EPR23474-76] (ab275876)
  • Anti-KRAS antibody [EPR23474-76] (ab275876)

Key features and details

  • Produced recombinantly (animal-free) for high batch-to-batch consistency and long term security of supply
  • Rabbit monoclonal [EPR23474-76] to KRAS
  • Suitable for: Flow Cyt (Intra), IP, WB
  • Reacts with: Human

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Overview

  • Product name

    Anti-KRAS antibody [EPR23474-76]
    See all KRAS primary antibodies
  • Description

    Rabbit monoclonal [EPR23474-76] to KRAS
  • Host species

    Rabbit
  • Specificity

    This antibody is not suitable for mouse or rat samples. 

  • Tested applications

    Suitable for: Flow Cyt (Intra), IP, WBmore details
    Unsuitable for: ICC or IHC-P
  • Species reactivity

    Reacts with: Human
    Does not react with: Mouse, Rat
  • Immunogen

    Recombinant fragment. This information is proprietary to Abcam and/or its suppliers.

  • Positive control

    • WB: Human brain tissue lysate; HeLa whole cell lysate; His-tagged human KRAS recombinant protein. Flow Cyt (intra): HeLa cells. IP: Human brain tissue lysate.
  • General notes

    This product is a recombinant monoclonal antibody, which offers several advantages including:

    • - High batch-to-batch consistency and reproducibility
    • - Improved sensitivity and specificity
    • - Long-term security of supply
    • - Animal-free production
    For more information see here.

    Our RabMAb® technology is a patented hybridoma-based technology for making rabbit monoclonal antibodies. For details on our patents, please refer to RabMAb® patents.

Properties

  • Form

    Liquid
  • Storage instructions

    Shipped at 4°C. Store at +4°C short term (1-2 weeks). Upon delivery aliquot. Store at -20°C long term. Avoid freeze / thaw cycle.
  • Storage buffer

    Preservative: 0.01% Sodium azide
    Constituents: 59.94% PBS, 40% Glycerol, 0.05% BSA
  • Concentration information loading...
  • Purity

    Protein A purified
  • Clonality

    Monoclonal
  • Clone number

    EPR23474-76
  • Isotype

    IgG
  • Research areas

    • Signal Transduction
    • Signaling Pathway
    • G Protein Signaling
    • Small G Proteins
    • Ras Family
    • Epigenetics and Nuclear Signaling
    • Transcription
    • Cancer susceptibility
    • Proto-oncogenes
    • Cancer
    • Signal transduction
    • G protein signaling
    • Small G proteins
    • Ras family

Associated products

  • Alternative Versions

    • Anti-KRAS antibody [EPR23474-76] - BSA and Azide free (ab275885)
  • Compatible Secondaries

    • Goat Anti-Rabbit IgG H&L (Alexa Fluor® 488) (ab150077)
    • Goat Anti-Rabbit IgG H&L (HRP) (ab97051)
  • Isotype control

    • Rabbit IgG, monoclonal [EPR25A] - Isotype Control (ab172730)

Applications

The Abpromise guarantee

Our Abpromise guarantee covers the use of ab275876 in the following tested applications.

The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.

Application Abreviews Notes
Flow Cyt (Intra)
1/50.
IP (2)
1/30.
WB (5)
1/1000. Detects a band of approximately 21 kDa (predicted molecular weight: 22 kDa).
Notes
Flow Cyt (Intra)
1/50.
IP
1/30.
WB
1/1000. Detects a band of approximately 21 kDa (predicted molecular weight: 22 kDa).
Application notes
Is unsuitable for ICC or IHC-P.

Target

  • Function

    Ras proteins bind GDP/GTP and possess intrinsic GTPase activity.
  • Involvement in disease

    Defects in KRAS are a cause of acute myelogenous leukemia (AML) [MIM:601626]. AML is a malignant disease in which hematopoietic precursors are arrested in an early stage of development.
    Defects in KRAS are a cause of juvenile myelomonocytic leukemia (JMML) [MIM:607785]. JMML is a pediatric myelodysplastic syndrome that constitutes approximately 30% of childhood cases of myelodysplastic syndrome (MDS) and 2% of leukemia. It is characterized by leukocytosis with tissue infiltration and in vitro hypersensitivity of myeloid progenitors to granulocyte-macrophage colony stimulating factor.
    Defects in KRAS are the cause of Noonan syndrome type 3 (NS3) [MIM:609942]. Noonan syndrome (NS) [MIM:163950] is a disorder characterized by dysmorphic facial features, short stature, hypertelorism, cardiac anomalies, deafness, motor delay, and a bleeding diathesis. It is a genetically heterogeneous and relatively common syndrome, with an estimated incidence of 1 in 1000-2500 live births. Rarely, NS is associated with juvenile myelomonocytic leukemia (JMML). NS3 inheritance is autosomal dominant.
    Defects in KRAS are a cause of gastric cancer (GASC) [MIM:613659]; also called gastric cancer intestinal or stomach cancer. Gastric cancer is a malignant disease which starts in the stomach, can spread to the esophagus or the small intestine, and can extend through the stomach wall to nearby lymph nodes and organs. It also can metastasize to other parts of the body. The term gastric cancer or gastric carcinoma refers to adenocarcinoma of the stomach that accounts for most of all gastric malignant tumors. Two main histologic types are recognized, diffuse type and intestinal type carcinomas. Diffuse tumors are poorly differentiated infiltrating lesions, resulting in thickening of the stomach. In contrast, intestinal tumors are usually exophytic, often ulcerating, and associated with intestinal metaplasia of the stomach, most often observed in sporadic disease.
    Note=Defects in KRAS are a cause of pylocytic astrocytoma (PA). Pylocytic astrocytomas are neoplasms of the brain and spinal cord derived from glial cells which vary from histologically benign forms to highly anaplastic and malignant tumors.
    Defects in KRAS are a cause of cardiofaciocutaneous syndrome (CFC syndrome) [MIM:115150]; also known as cardio-facio-cutaneous syndrome. CFC syndrome is characterized by a distinctive facial appearance, heart defects and mental retardation. Heart defects include pulmonic stenosis, atrial septal defects and hypertrophic cardiomyopathy. Some affected individuals present with ectodermal abnormalities such as sparse, friable hair, hyperkeratotic skin lesions and a generalized ichthyosis-like condition. Typical facial features are similar to Noonan syndrome. They include high forehead with bitemporal constriction, hypoplastic supraorbital ridges, downslanting palpebral fissures, a depressed nasal bridge, and posteriorly angulated ears with prominent helices. The inheritance of CFC syndrome is autosomal dominant.
    Note=KRAS mutations are involved in cancer development.
  • Sequence similarities

    Belongs to the small GTPase superfamily. Ras family.
  • Cellular localization

    Cell membrane.
  • Target information above from: UniProt accession P01116 The UniProt Consortium
    The Universal Protein Resource (UniProt) in 2010
    Nucleic Acids Res. 38:D142-D148 (2010) .

    Information by UniProt
  • Database links

    • Entrez Gene: 3845 Human
    • Omim: 190070 Human
    • SwissProt: P01116 Human
    • Unigene: 37003 Human
    • Unigene: 505033 Human
    • Alternative names

      • c Ki ras2 antibody
      • c Kirsten ras protein antibody
      • c-K-ras antibody
      • c-Ki-ras antibody
      • Cellular c Ki ras2 proto oncogene antibody
      • Cellular transforming proto oncogene antibody
      • CFC2 antibody
      • cK Ras antibody
      • GTPase KRas antibody
      • K RAS p21 protein antibody
      • K RAS2A antibody
      • K RAS2B antibody
      • K RAS4A antibody
      • K RAS4B antibody
      • K-Ras 2 antibody
      • KI RAS antibody
      • Ki-Ras antibody
      • KIRSTEN MURINE SARCOMA VIRUS 2 antibody
      • Kirsten rat sarcoma 2 viral (v Ki ras2) oncogene homolog antibody
      • Kirsten rat sarcoma viral oncogene homolog antibody
      • KRAS antibody
      • KRAS proto oncogene, GTPase antibody
      • KRAS1 antibody
      • KRAS2 antibody
      • N-terminally processed antibody
      • NS antibody
      • NS3 antibody
      • Oncogene KRAS2 antibody
      • p21ras antibody
      • PR310 c K ras oncogene antibody
      • PR310 cK ras oncogene antibody
      • RALD antibody
      • RASK_HUMAN antibody
      • RASK2 antibody
      • Transforming protein p21 antibody
      • v Ki ras2 Kirsten rat sarcoma 2 viral oncogene homolog antibody
      • v Ki ras2 Kirsten rat sarcoma viral oncogene homolog antibody
      see all

    Images

    • Western blot - Anti-KRAS antibody [EPR23474-76] (ab275876)
      Western blot - Anti-KRAS antibody [EPR23474-76] (ab275876)
      All lanes : Anti-KRAS antibody [EPR23474-76] (ab275876) at 1/1000 dilution

      Lane 1 : Human brain tissue lysate
      Lane 2 : HeLa (human cervix adenocarcinoma epithelial cell) whole cell lysate

      Lysates/proteins at 40 µg per lane.

      Secondary
      All lanes : Goat Anti-Rabbit IgG, (H+L), Peroxidase conjugated (ab97051) at 1/50000 dilution

      Predicted band size: 22 kDa
      Observed band size: 21 kDa why is the actual band size different from the predicted?



      Blocking and diluting buffer and concentration: 5% NFDM/TBST.

      Exposure time: 3 minutes

       

    • Flow Cytometry (Intracellular) - Anti-KRAS antibody [EPR23474-76] (ab275876)
      Flow Cytometry (Intracellular) - Anti-KRAS antibody [EPR23474-76] (ab275876)

      Intracellular flow cytometric analysis of 4% paraformaldehyde-fixed, 90% methanol-permeabilized HeLa (human cervix adenocarcinoma epithelial cell) cells labelling KRAS with ab275876 at 1/50 dilution (1ug)/ (Red) compared with a Rabbit monoclonal IgG (ab172730) isotype control (Black) and an unlabelled control (cells without incubation with primary antibody and secondary antibody) (Blue). 

      A Goat anti rabbit IgG (Alexa Fluor® 488, ab150077) at 1/2000 dilution was used as the secondary antibody. 

       

       

    • Immunoprecipitation - Anti-KRAS antibody [EPR23474-76] (ab275876)
      Immunoprecipitation - Anti-KRAS antibody [EPR23474-76] (ab275876)

      KRAS was immunoprecipitated from 0.35 mg Human brain tissue lysate with ab275876 at 1/30 dilution (2ug in 0.35mg lysates). Western blot was performed on the immunoprecipitate using ab275876 at 1/1000 dilution. VeriBlot for IP Detection Reagent (HRP)(ab131366) was used at 1/5000 dilution.

      Lane 1: Human brain tissue lysate 10 ug

      Lane 2: ab275876 IP in Human brain tissue lysate

      Lane 3: Rabbit monoclonal IgG (ab172730) instead of ab275876 in human brain tissue lysate

      Blocking and dilution buffer and concentration: 5% NFDM/TBST.

      Exposure time: 3 minutes.

       

    • Western blot - Anti-KRAS antibody [EPR23474-76] (ab275876)
      Western blot - Anti-KRAS antibody [EPR23474-76] (ab275876)
      All lanes : Anti-KRAS antibody [EPR23474-76] (ab275876) at 1/1000 dilution

      Lane 1 : His-tagged human KRAS recombinant protein, 10 ng
      Lane 2 : His-tagged human HRAS recombinant protein (aa 1-186), 20 ng
      Lane 3 : His-tagged human NRAS recombinant protein (aa 1-186), 20 ng

      Secondary
      All lanes : Goat Anti-Rabbit IgG, (H+L), Peroxidase conjugated (ab97051) at 1/100000 dilution

      Predicted band size: 22 kDa
      Observed band size: 21 kDa why is the actual band size different from the predicted?



      Blocking and diluting buffer and concentration: 5% NFDM/TBST.

      Exposure time: 3 minutes.

       

    • Anti-KRAS antibody [EPR23474-76] (ab275876)
      Anti-KRAS antibody [EPR23474-76] (ab275876)

    Protocols

    To our knowledge, customised protocols are not required for this product. Please try the standard protocols listed below and let us know how you get on.

    Click here to view the general protocols

    Datasheets and documents

    • Datasheet download

      Download

    Certificate of Compliance

    To download a Certificate of Compliance, please enter your Lot number below:

    References (1)

    Publishing research using ab275876? Please let us know so that we can cite the reference in this datasheet.

    ab275876 has been referenced in 1 publication.

    • Hu Q  et al. The Promotional Effect of Hollow MnO2 with Brucea Javanica Oil Emulsion (BJOE) on Endometrial Cancer Apoptosis. Biomed Res Int 2021:6631533 (2021). PubMed: 33816622

    Customer reviews and Q&As

    Show All Reviews Q&A
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    1-5 of 5 Abreviews or Q&A

    Western blot abreview for Anti-KRAS antibody [EPR23474-76]

    Good
    Abreviews
    Abreviews
    abreview image
    Application
    Western blot
    Sample
    Monkey Cell lysate - whole cell (COS-1 cells)
    Gel Running Conditions
    Reduced Denaturing (4-15%)
    Loading amount
    25 µg
    Specification
    COS-1 cells
    Blocking step
    Milk as blocking agent for 1 hour(s) and 0 minute(s) · Concentration: 5% · Temperature: 25°C
    Read More

    Abcam user community

    Verified customer

    Submitted May 05 2022

    Western blot abreview for Anti-KRAS antibody [EPR23474-76]

    Excellent
    Abreviews
    Abreviews
    This product is known to not work in this application or species.
    abreview image
    Application
    Western blot
    Sample
    Rat Cell lysate - whole cell (3611-RF cells)
    Gel Running Conditions
    Reduced Denaturing (4-15%)
    Loading amount
    25 µg
    Specification
    3611-RF cells
    Blocking step
    Milk as blocking agent for 1 hour(s) and 0 minute(s) · Concentration: 5% · Temperature: 25°C
    Read More

    Abcam user community

    Verified customer

    Submitted May 05 2022

    Western blot abreview for Anti-KRAS antibody [EPR23474-76]

    Excellent
    Abreviews
    Abreviews
    abreview image
    Application
    Western blot
    Sample
    Dog Cell lysate - whole cell (MDCK cells)
    Gel Running Conditions
    Reduced Denaturing (4-15%)
    Loading amount
    25 µg
    Specification
    MDCK cells
    Blocking step
    Milk as blocking agent for 1 hour(s) and 0 minute(s) · Concentration: 5% · Temperature: 25°C
    Read More

    Abcam user community

    Verified customer

    Submitted May 05 2022

    Western blot abreview for Anti-KRAS antibody [EPR23474-76]

    Poor
    Abreviews
    Abreviews
    This product is known to not work in this application or species.
    abreview image
    Application
    Western blot
    Sample
    Mouse Cell lysate - whole cell (MEF)
    Gel Running Conditions
    Reduced Denaturing (12%)
    Loading amount
    25 µg
    Specification
    MEF
    Blocking step
    Milk as blocking agent for 1 hour(s) and 0 minute(s) · Concentration: 5% · Temperature: 25°C
    Read More

    Abcam user community

    Verified customer

    Submitted Jan 26 2021

    Western blot abreview for Anti-KRAS antibody [EPR23474-76]

    Excellent
    Abreviews
    Abreviews
    abreview image
    Application
    Western blot
    Sample
    Human Cell lysate - whole cell (PANC-1 cells)
    Gel Running Conditions
    Reduced Denaturing (12%)
    Loading amount
    20 µg
    Specification
    PANC-1 cells
    Blocking step
    BSA as blocking agent for 1 hour(s) and 0 minute(s) · Concentration: 5% · Temperature: 25°C
    Read More

    Abcam user community

    Verified customer

    Submitted Jan 18 2021

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