Storage instructionsShipped at 4°C. Upon delivery aliquot and store at -20°C or -80°C. Avoid repeated freeze / thaw cycles.
Storage bufferPreservative: None
PBS, pH 7.2
Concentration information loading...
PurityProtein G purified
Light chain typekappa
Our Abpromise guarantee covers the use of ab55391 in the following tested applications.
The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.
|WB||Use a concentration of 1 - 5 µg/ml. Predicted molecular weight: 22 kDa.|
|IHC-P||Use at an assay dependent concentration.|
|Flow Cyt||Use 0.5-1µg for 106 cells.
ab170191 - Mouse monoclonal IgG2a, is suitable for use as an isotype control with this antibody.
RelevanceRas proteins bind GDP/GTP and possess intrinsic GTPase activity. Plays an important role in the regulation of cell proliferation. Enzyme regulation: Alternates between an inactive form bound to GDP and an active form bound to GTP. Activated by a guanine nucleotide-exchange factor (GEF) and inactivated by a GTPase-activating protein (GAP). Interaction with SOS1 promotes exchange of bound GDP by GTP.
Cellular localizationCell Membrane, Cytoplasmic and Golgi Apparatus
- c-H-ras antibody
- c-K-ras antibody
- c-Ki-ras antibody
cK Ras antibody (ab55391) at 1ug/lane + HeLa cell lysate at 25ug/lane.
Overlay histogram showing HeLa cells stained with ab55391 (red line). The cells were fixed with 4% paraformaldehyde (10 min) and then permeabilized with 0.1% PBS-Tween for 20 min. The cells were then incubated in 1x PBS / 10% normal goat serum / 0.3M glycine to block non-specific protein-protein interactions followed by the antibody (ab55391, 1µg/1x106 cells) for 30 min at 22°C. The secondary antibody used was DyLight® 488 goat anti-mouse IgG (H+L) (ab96879) at 1/500 dilution for 30 min at 22°C. Isotype control antibody (black line) was mouse IgG2a [ICIGG2A] (ab91361, 1µg/1x106 cells) used under the same conditions. Acquisition of >5,000 events was performed. This antibody gave a slightly decreased signal in HeLa cells fixed with methanol (5 min)/permeabilized with 0.1% PBS-Tween 20 used under the same conditions.
ab55391 staining KRAS+HRAS+NRAS in Human Gastric Acenocarcinoma tissue sections by Immunohistochemistry (IHC-P - paraformaldehyde-fixed, paraffin-embedded sections). Tissue was fixed with 10% phosphate buffered formalin, permeabilized with 0.025% of triton X -100 in TBS and blocked with 3% hydrogen peroxide for 5 minutes at room temperature; antigen retrieval was by heat mediation in target retrieval solution. Samples were incubated with primary antibody (1/100 in 0.025% triton X-100-TBS) for 1 hour. An HRP polymer was used for detection.
This product has been referenced in:
- Zhang L et al. Loss of ARID1A Expression Correlates With Tumor Differentiation and Tumor Progression Stage in Pancreatic Ductal Adenocarcinoma. Technol Cancer Res Treat 17:1533034618754475 (2018). Read more (PubMed: 29486633) »
- Bäumer N et al. Downregulation of PIK3CA via antibody-esiRNA-complexes suppresses human xenograft tumor growth. PLoS One 13:e0200163 (2018). Read more (PubMed: 30001368) »