Anti-KRIT1 antibody (ab110530)

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Western blot - Anti-KRIT1 antibody (ab110530)

    Key features and details

    • Rabbit polyclonal to KRIT1
    • Suitable for: WB
    • Reacts with: Human
    • Isotype: IgG

    Overview

    • Product name

      Anti-KRIT1 antibody
      See all KRIT1 primary antibodies

    • Description

      Rabbit polyclonal to KRIT1

    • Host species

      Rabbit

    • Tested applications

      Suitable for: WBmore details

    • Species reactivity

      Reacts with: Human
      Predicted to work with: Mouse, Rat, Dog, Pig, Chimpanzee, Macaque monkey, Gorilla

    • Immunogen

      Synthetic peptide corresponding to Human KRIT1 aa 700 to the C-terminus (C terminal) conjugated to keyhole limpet haemocyanin.
      (Peptide available as ab130766)

    • Positive control

      • This antibody gave a positive signal in Raji and SHSY5Y whole cell lysates.

    • General notes

      Reproducibility is key to advancing scientific discovery and accelerating scientists’ next breakthrough.

      Abcam is leading the way with our range of recombinant antibodies, knockout-validated antibodies and knockout cell lines, all of which support improved reproducibility.

      We are also planning to innovate the way in which we present recommended applications and species on our product datasheets, so that only applications & species that have been tested in our own labs, our suppliers or by selected trusted collaborators are covered by our Abpromise™ guarantee.

      In preparation for this, we have started to update the applications & species that this product is Abpromise guaranteed for.

      We are also updating the applications & species that this product has been “predicted to work with,” however this information is not covered by our Abpromise guarantee.

      Applications & species from publications and Abreviews that have not been tested in our own labs or in those of our suppliers are not covered by the Abpromise guarantee.

      Please check that this product meets your needs before purchasing. If you have any questions, special requirements or concerns, please send us an inquiry and/or contact our Support team ahead of purchase. Recommended alternatives for this product can be found below, as well as customer reviews and Q&As.

    Properties

    Applications

    Our Abpromise guarantee covers the use of ab110530 in the following tested applications.

    The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.

    Application Abreviews Notes
    WB Use a concentration of 1 µg/ml. Detects a band of approximately 84 kDa (predicted molecular weight: 84 kDa).

    Target

    • Function

      Negative regulator of angiogenesis. Inhibits endothelial proliferation, apoptosis, migration, lumen formation and sprouting angiogenesis in primary endothelial cells. Promotes AKT phosphorylation in a NOTCH-dependent and independent manner, and inhibits EKR1/2 phosphorylation indirectly through activation of the DELTA-NOTCH cascade. Acts in concert with CDH5 to establish and maintain correct endothelial cell polarity and vascular lumen and these effects are mediated by recruitment and activation of the Par polarity complex and RAP1B. Required for the localization of phosphorylated PRKCZ, PARD3, TIAM1 and RAP1B to the cell junction. Plays an important role in the maintenance of the intracellular reactive oxygen species (ROS) homeostasis to prevent oxidative cellular damage. Regulates the homeostasis of intracellular ROS through an antioxidant pathway involving FOXO1 and SOD2. Facilitates the down-regulation of cyclin D1 levels required for cell transition from proliferative growth to quiescence by preventing the accumulation of intracellular ROS through the modulation of FOXO1 and SOD2 levels.

    • Tissue specificity

      Low levels in brain. Very weak expression found in heart and muscle.

    • Involvement in disease

      Defects in KRIT1 are the cause of cerebral cavernous malformations type 1 (CCM1) [MIM:116860]. Cerebral cavernous malformations (CCMs) are congenital vascular anomalies of the central nervous system that can result in hemorrhagic stroke, seizures, recurrent headaches, and focal neurologic deficits. CCMs have an incidence of 0.1%-0.5% in the general population and usually present clinically during the 3rd to 5th decade of life. The lesions are characterized by grossly enlarged blood vessels consisting of a single layer of endothelium and without any intervening neural tissue, ranging in diameter from a few millimeters to several centimeters.

    • Sequence similarities

      Contains 4 ANK repeats.
      Contains 1 FERM domain.

    • Cellular localization

      Membrane. Cell junction. KRIT1 and CDH5 reciprocally regulate their localization to endothelial cell-cell junctions.
    • Information by UniProt

    • Database links

      see all

    • Alternative names

      • Ankyrin repeat containing protein Krit1 antibody
      • CAM antibody
      • CCM 1 antibody
      • CCM1 antibody
      • Cerebral cavernous malformations 1 antibody
      • Cerebral cavernous malformations 1 protein antibody
      • Krev interaction trapped 1 antibody
      • Krev interaction trapped protein 1 antibody
      • KRIT 1 antibody
      • KRIT1 ankyrin repeat containing antibody
      • KRIT1 antibody
      • KRIT1_HUMAN antibody
      see all

    Images

    • Western blot - Anti-KRIT1 antibody (ab110530)
      Western blot - Anti-KRIT1 antibody (ab110530)
      All lanes : Anti-KRIT1 antibody (ab110530) at 1 µg/ml

      Lane 1 : Raji (Human Burkitt's lymphoma cell line) Whole Cell Lysate
      Lane 2 : SHSY-5Y (Human neuroblastoma cell line) Whole Cell Lysate

      Lysates/proteins at 10 µg per lane.

      Secondary
      All lanes : Goat Anti-Rabbit IgG H&L (HRP) preadsorbed (ab97080) at 1/5000 dilution

      Developed using the ECL technique.

      Performed under reducing conditions.

      Predicted band size: 84 kDa
      Observed band size: 84 kDa


      Exposure time: 20 minutes

    References (0)

    Publishing research using ab110530? Please let us know so that we can cite the reference in this datasheet.

    ab110530 has not yet been referenced specifically in any publications.

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