The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.
Use a concentration of 1 µg/ml. Predicted molecular weight: 55 kDa. Good results were obtained when blocked with 5% non-fat dry milk in 0.05% PBS-T.
KRT24 is a member of the type I (acidic) keratin family, which belongs to the superfamily of intermediate filament (IF) proteins. Keratins are heteropolymeric structural proteins which form the intermediate filament. These filaments, along with actin microfilaments and microtubules, comprise the cytoskeleton of epithelial cells. KRT24 is highly expressed in keratinocytes, placenta, colon and spleen, and has been found to be up-regulated in the mucosa of patients suffering of colorectal cancer.