Key features and details
- Rabbit polyclonal to KRT6B
- Suitable for: WB, IHC-P, ICC/IF
- Reacts with: Mouse, Human
- Isotype: IgG
Product nameAnti-KRT6B antibody
See all KRT6B primary antibodies
DescriptionRabbit polyclonal to KRT6B
Tested applicationsSuitable for: WB, IHC-P, ICC/IFmore details
Species reactivityReacts with: Mouse, Human
Recombinant fragment corresponding to a region within amino acids 134-564 of Human KRT6B.
- HeLa whole cell lysate. HeLa cells. Mouse brain tissue.
General notesKeep as concentrated solution.
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Abcam is leading the way with our range of recombinant antibodies, knockout-validated antibodies and knockout cell lines, all of which support improved reproducibility.
We are also planning to innovate the way in which we present recommended applications and species on our product datasheets, so that only applications & species that have been tested in our own labs, our suppliers or by selected trusted collaborators are covered by our Abpromise™ guarantee.
In preparation for this, we have started to update the applications & species that this product is Abpromise guaranteed for.
We are also updating the applications & species that this product has been “predicted to work with,” however this information is not covered by our Abpromise guarantee.
Applications & species from publications and Abreviews that have not been tested in our own labs or in those of our suppliers are not covered by the Abpromise guarantee.
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Storage instructionsShipped at 4°C. Upon delivery aliquot. Store at -20°C or -80°C. Avoid freeze / thaw cycle.
Storage bufferpH: 7.00
Preservative: 0.01% Thimerosal (merthiolate)
Constituents: 79.99% PBS, 20% Glycerol (glycerin, glycerine)
Concentration information loading...
PurityImmunogen affinity purified
Our Abpromise guarantee covers the use of ab154313 in the following tested applications.
The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.
|WB||1/1000 - 1/10000. Predicted molecular weight: 60 kDa.|
|IHC-P||1/100 - 1/1000. Perform heat mediated antigen retrieval with citrate buffer pH 6 before commencing with IHC staining protocol.|
|ICC/IF||1/100 - 1/1000.|
Tissue specificityConstitutively expressed in distinct types of epithelia such as those in oral mucosa, esophagus, papillae of tongue and hair follicle outer root sheath.
Involvement in diseaseDefects in KRT6B are a cause of pachyonychia congenita type 2 (PC2) [MIM:167210]; also known as pachyonychia congenita Jackson-Lawler type. PC2 is an autosomal dominant ectodermal dysplasia characterized by hypertrophic nail dystrophy resulting in onchyogryposis (thickening and increase in curvature of the nail), palmoplantar keratoderma and hyperhidrosis, follicular hyperkeratosis, multiple epidermal cysts, absent/sparse eyebrow and body hair, and by the presence of natal teeth.
Sequence similaritiesBelongs to the intermediate filament family.
- Information by UniProt
- CK-6B antibody
- CK6B antibody
- Cytokeratin-6B antibody
Immunohistochemical analysis of paraffin-embedded Mouse brain tissue labeling KRT6B using ab154313 at 1/500 dilution.
Anti-KRT6B antibody (ab154313) at 1/5000 dilution + HeLa cell lysate at 1 µg
Predicted band size: 60 kDa
7.5% SDS PAGE
Immunofluorescence analysis of methanol-fixed HeLa cells, labeling KRT6B using ab154313 at 1/500 dilution. Right panel costained with Hoechst 33342.
ab154313 has not yet been referenced specifically in any publications.