Overview

  • Product name

  • Description

    Rabbit polyclonal to KRT6B
  • Host species

    Rabbit
  • Tested applications

    Suitable for: WB, IHC-P, ICC/IFmore details
  • Species reactivity

    Reacts with: Mouse, Human
  • Immunogen

    Recombinant fragment corresponding to a region within amino acids 134-564 of Human KRT6B.

  • Positive control

    • HeLa whole cell lysate. HeLa cells. Mouse brain tissue.
  • General notes

    Keep as concentrated solution.

Properties

Applications

Our Abpromise guarantee covers the use of ab154313 in the following tested applications.

The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.

Application Abreviews Notes
WB 1/1000 - 1/10000. Predicted molecular weight: 60 kDa.
IHC-P 1/100 - 1/1000. Perform heat mediated antigen retrieval with citrate buffer pH 6 before commencing with IHC staining protocol.
ICC/IF 1/100 - 1/1000.

Target

  • Tissue specificity

    Constitutively expressed in distinct types of epithelia such as those in oral mucosa, esophagus, papillae of tongue and hair follicle outer root sheath.
  • Involvement in disease

    Defects in KRT6B are a cause of pachyonychia congenita type 2 (PC2) [MIM:167210]; also known as pachyonychia congenita Jackson-Lawler type. PC2 is an autosomal dominant ectodermal dysplasia characterized by hypertrophic nail dystrophy resulting in onchyogryposis (thickening and increase in curvature of the nail), palmoplantar keratoderma and hyperhidrosis, follicular hyperkeratosis, multiple epidermal cysts, absent/sparse eyebrow and body hair, and by the presence of natal teeth.
  • Sequence similarities

    Belongs to the intermediate filament family.
  • Information by UniProt
  • Database links

  • Alternative names

    • CK-6B antibody
    • CK6B antibody
    • Cytokeratin-6B antibody
    • K2C6B_HUMAN antibody
    • K6b antibody
    • Keratin antibody
    • Keratin epidermal type II K6B antibody
    • Keratin like 1 (a type II keratin sequence) antibody
    • Keratin type II cytoskeletal 6B antibody
    • Keratin-6B antibody
    • KRT6B antibody
    • KRTL1 antibody
    • PC2 antibody
    • PC4 antibody
    • type II cytoskeletal 6B antibody
    • Type-II keratin Kb10 antibody
    see all

Images

  • Immunohistochemical analysis of paraffin-embedded Mouse brain tissue labeling KRT6B using ab154313 at 1/500 dilution.
  • Anti-KRT6B antibody (ab154313) at 1/5000 dilution + HeLa cell lysate at 1 µg

    Predicted band size: 60 kDa



    7.5% SDS PAGE
  • Immunofluorescence analysis of methanol-fixed HeLa cells, labeling KRT6B using ab154313 at 1/500 dilution. Right panel costained with Hoechst 33342.

References

ab154313 has not yet been referenced specifically in any publications.

Customer reviews and Q&As

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