RecombinantRabMAb

Recombinant Anti-KRT6C antibody [SP87] - BSA and Azide free (ab240985)

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Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-KRT6C antibody [SP87] - BSA and Azide free (ab240985)
  • Immunocytochemistry - Anti-KRT6C antibody [SP87] - BSA and Azide free (ab240985)
  • Flow Cytometry - Anti-KRT6C antibody [SP87] - BSA and Azide free (ab240985)
  • Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-KRT6C antibody [SP87] - BSA and Azide free (ab240985)
  • Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-KRT6C antibody [SP87] - BSA and Azide free (ab240985)
  • Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-KRT6C antibody [SP87] - BSA and Azide free (ab240985)
  • Immunohistochemistry (Frozen sections) - Anti-KRT6C antibody [SP87] - BSA and Azide free (ab240985)
  • Immunohistochemistry (Frozen sections) - Anti-KRT6C antibody [SP87] - BSA and Azide free (ab240985)
  • Flow Cytometry - Anti-KRT6C antibody [SP87] - BSA and Azide free (ab240985)
  • Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-KRT6C antibody [SP87] - BSA and Azide free (ab240985)
  • Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-KRT6C antibody [SP87] - BSA and Azide free (ab240985)
  • Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-KRT6C antibody [SP87] - BSA and Azide free (ab240985)
  • Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-KRT6C antibody [SP87] - BSA and Azide free (ab240985)
  • Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-KRT6C antibody [SP87] - BSA and Azide free (ab240985)
  • Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-KRT6C antibody [SP87] - BSA and Azide free (ab240985)
  • Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-KRT6C antibody [SP87] - BSA and Azide free (ab240985)
  • Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-KRT6C antibody [SP87] - BSA and Azide free (ab240985)
  • Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-KRT6C antibody [SP87] - BSA and Azide free (ab240985)
  • Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-KRT6C antibody [SP87] - BSA and Azide free (ab240985)
  • Anti-KRT6C antibody [SP87] - BSA and Azide free (ab240985)

Key features and details

  • Produced recombinantly (animal-free) for high batch-to-batch consistency and long term security of supply
  • Rabbit monoclonal [SP87] to KRT6C - BSA and Azide free
  • Suitable for: ICC, IHC-Fr, Flow Cyt, WB, IHC-P
  • Reacts with: Mouse, Rat, Human

Overview

  • Product name

    Anti-KRT6C antibody [SP87] - BSA and Azide free
    See all KRT6C primary antibodies

  • Description

    Rabbit monoclonal [SP87] to KRT6C - BSA and Azide free

  • Host species

    Rabbit

  • Tested applications

    Suitable for: ICC, IHC-Fr, Flow Cyt, WB, IHC-Pmore details

  • Species reactivity

    Reacts with: Mouse, Rat, Human
    Predicted to work with: Cow

  • Immunogen

    Synthetic peptide within Human KRT6C aa 550 to the C-terminus (C terminal). The exact sequence is proprietary.
    Database link: P48668

  • Positive control

    • WB: A431 whole cell lysate. IHC-P: Human prostate carcinoma, breast, stomach, prostate, lung squamous cell carcinoma, rectal adenocarcinoma, bladder, bladder transitional cell carcinoma, colon adenocarcinoma, and placenta tissue, rat and mouse skin Flow Cyt: A431 and HaCaT cells. ICC/IF: HaCaT cells. IHC-Fr: Rat and mouse skin tissue.

  • General notes

    FOR RESEARCH USE ONLY. For commercial use, please contact partnerships@abcam.com.

    ab240985 is the carrier-free version of ab227643. This format is designed for use in antibody labeling, including fluorochromes, metal isotopes, oligonucleotides, enzymes.

    Our carrier-free formats are supplied in a buffer free of BSA, sodium azide and glycerol for higher conjugation efficiency.

    Use our conjugation kits for antibody conjugates that are ready-to-use in as little as 20 minutes with <1 minute hands-on-time and 100% antibody recovery: available for fluorescent dyes, HRP, biotin and gold.

    ab240985 is compatible with the Maxpar® Antibody Labeling Kit from Fluidigm.

    Maxpar® is a trademark of Fluidigm Canada Inc.

    This product is a recombinant monoclonal antibody, which offers several advantages including:

    • - High batch-to-batch consistency and reproducibility
    • - Improved sensitivity and specificity
    • - Long-term security of supply
    • - Animal-free production
    For more information see here.

    Reproducibility is key to advancing scientific discovery and accelerating scientists’ next breakthrough.

    Abcam is leading the way with our range of recombinant antibodies, knockout-validated antibodies and knockout cell lines, all of which support improved reproducibility.

    We are also planning to innovate the way in which we present recommended applications and species on our product datasheets, so that only applications & species that have been tested in our own labs, our suppliers or by selected trusted collaborators are covered by our Abpromise™ guarantee.

    In preparation for this, we have started to update the applications & species that this product is Abpromise guaranteed for.

    We are also updating the applications & species that this product has been “predicted to work with,” however this information is not covered by our Abpromise guarantee.

    Applications & species from publications and Abreviews that have not been tested in our own labs or in those of our suppliers are not covered by the Abpromise guarantee.

    Please check that this product meets your needs before purchasing. If you have any questions, special requirements or concerns, please send us an inquiry and/or contact our Support team ahead of purchase. Recommended alternatives for this product can be found below, as well as customer reviews and Q&As.

Properties

Applications

Our Abpromise guarantee covers the use of ab240985 in the following tested applications.

The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.

Application Abreviews Notes
ICC Use at an assay dependent concentration.
IHC-Fr Use at an assay dependent concentration.
Flow Cyt Use at an assay dependent concentration.

Primary antibody incubation for 30 minutes at 4 degrees.

 
WB Use at an assay dependent concentration. Predicted molecular weight: 60 kDa.
IHC-P 1/200.

Boil tissue section in 10mM citrate, pH 6.0 for 10 min followed by cooling at room temperature for 20 min. Incubate with ab227643 for 10 minutes at room temperature.

Target

  • Tissue specificity

    Constitutively expressed in distinct types of epithelia such as those in oral mucosa, esophagus, papillae of tongue and hair follicle outer root sheath.

  • Sequence similarities

    Belongs to the intermediate filament family.
  • Information by UniProt

  • Database links

    • Alternative names

      • CK-6C antibody
      • CK-6E antibody
      • Cytokeratin-6C antibody
      • Cytokeratin-6E antibody
      • K2C6C_HUMAN antibody
      • K6C antibody
      • K6E antibody
      • keratin 6C antibody
      • Keratin antibody
      • Keratin K6h antibody
      • Keratin type II cytoskeletal 6C antibody
      • Keratin-6C antibody
      • KRT6C antibody
      • KRT6E antibody
      • type II cytoskeletal 6C antibody
      • Type-II keratin Kb12 antibody
      see all

    Images

    • Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-KRT6C antibody [SP87] - BSA and Azide free (ab240985)
      Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-KRT6C antibody [SP87] - BSA and Azide free (ab240985)

      This data was developed using the same antibody clone in a different buffer formulation (ab227643).

      Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) analysis of Human prostate carcinoma tissue sections labeling KRT6C with ab227643 at 1/200 dilution (0.12 μg/ml). Heat mediated antigen retrieval with sodium citrate buffer (pH 6.0, epitope retrieval solution 1) for 10mins. Leica DS9800 (Bond™ Polymer Refine Detection) was used as a secondary antibody. Hematoxylin was used as a counterstain. Positive staining on the human prostate carcinoma, performed on a Leica Biosystems BOND™ RX instrument. 
      The section was incubated with ab227643 for 10 mins at room temperature.

    • Immunocytochemistry - Anti-KRT6C antibody [SP87] - BSA and Azide free (ab240985)
      Immunocytochemistry - Anti-KRT6C antibody [SP87] - BSA and Azide free (ab240985)

      Immunocytochemistry/ Immunofluorescence analysis of HaCaT (human skin keratinocyte) cells labeling KRT6C with purified ab227643 at 1/50 (1.92 µg/ml). Cells were fixed in 100% Methanol. Cells were counterstained with ab195889 Anti-alpha Tubulin antibody [DM1A] - Microtubule Marker (Alexa Fluor® 594) 1/200 (2.5 µg/ml). Goat anti rabbit IgG (Alexa Fluor® 488, ab150077) was used as the secondary antibody at 1/1000 (2 µg/ml) dilution. DAPI was used as nuclear counterstain. PBS instead of the primary antibody was used as the secondary antibody only control.

      This data was developed using the same antibody clone in a different buffer formulation containing PBS, BSA, glycerol, and sodium azide (ab227643).

    • Flow Cytometry - Anti-KRT6C antibody [SP87] - BSA and Azide free (ab240985)
      Flow Cytometry - Anti-KRT6C antibody [SP87] - BSA and Azide free (ab240985)

      Flow cytometry analysis of HaCaT (human skin keratinocyte) labeling KRT6C with purified ab227643 at 1/200 dilution (0.48 µg/ml) (red). Cells were fixed with 4% paraformaldehyde and permeabilised with 90% methanol. Goat anti rabbit IgG (Alexa Fluor® 488, ab150077) at 1/2000 dilution was used as a secondary antibody. Isotype control - Rabbit monoclonal IgG (ab172730) (black). Unlableled control - Unlabelled cells (blue).

      This data was developed using the same antibody clone in a different buffer formulation containing PBS, BSA, glycerol, and sodium azide (ab227643).

    • Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-KRT6C antibody [SP87] - BSA and Azide free (ab240985)
      Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-KRT6C antibody [SP87] - BSA and Azide free (ab240985)

      This data was developed using the same antibody clone in a different buffer formulation (ab227643).

      Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) analysis of Rat skin tissue sections labeling KRT6C with ab227643 at 1/200 dilution (0.12 μg/ml). Heat mediated antigen retrieval with sodium citrate buffer (pH 6.0, epitope retrieval solution 1) for 10mins. Leica DS9800 (Bond™ Polymer Refine Detection) was used as a secondary antibody. Hematoxylin was used as a counterstain. Positive staining on the rat skin, performed on a Leica Biosystems BOND™ RX instrument. 
      The section was incubated with ab227643 for 10 mins at room temperature.

    • Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-KRT6C antibody [SP87] - BSA and Azide free (ab240985)
      Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-KRT6C antibody [SP87] - BSA and Azide free (ab240985)

      This data was developed using the same antibody clone in a different buffer formulation (ab227643).

      Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) analysis of Mouse skin tissue sections labeling KRT6C with ab227643 at 1/200 dilution (0.12 μg/ml). Heat mediated antigen retrieval with sodium citrate buffer (pH 6.0, epitope retrieval solution 1) for 10mins. Leica DS9800 (Bond™ Polymer Refine Detection) was used as a secondary antibody. Hematoxylin was used as a counterstain. Positive staining on the mouse skin, performed on a Leica Biosystems BOND™ RX instrument. 
      The section was incubated with ab227643 for 10 mins at room temperature.

    • Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-KRT6C antibody [SP87] - BSA and Azide free (ab240985)
      Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-KRT6C antibody [SP87] - BSA and Azide free (ab240985)

      Formalin-fixed, paraffin-embedded human prostate carcinoma tissue stained for KRT6C using ab227643 at 1/200 dilution in immunohistochemical analysis.

      This data was developed using the same antibody clone in a different buffer formulation containing PBS, BSA, glycerol, and sodium azide (ab227643)
    • Immunohistochemistry (Frozen sections) - Anti-KRT6C antibody [SP87] - BSA and Azide free (ab240985)
      Immunohistochemistry (Frozen sections) - Anti-KRT6C antibody [SP87] - BSA and Azide free (ab240985)

      Immunohistochemistry (Frozen) analysis of rat skin tissue section labeling KRT6C with purified ab227643 at 1/25 (3.84µg/ml). Sections were fixed in 4% paraformaldehyde and permeabilized with 0.2% Triton X-100. Antigen retrieval was Heat mediated antigen retrieval using sodium citrate buffer (10mM citrate pH 6.0 + 0.05% Tween-20). Goat anti rabbit IgG (Alexa Fluor® 488, ab150077) was used as the secondary antibody at 1/1000 (2 µg/ml) dilution. DAPI was used as nuclear counterstain. PBS instead of the primary antibody was used as the secondary antibody only control.

      This data was developed using the same antibody clone in a different buffer formulation containing PBS, BSA, glycerol, and sodium azide (ab227643).

    • Immunohistochemistry (Frozen sections) - Anti-KRT6C antibody [SP87] - BSA and Azide free (ab240985)
      Immunohistochemistry (Frozen sections) - Anti-KRT6C antibody [SP87] - BSA and Azide free (ab240985)

      Immunohistochemistry (Frozen) analysis of mouse skin tissue section labeling KRT6C with purified ab227643 at 1/25 (3.84µg/ml). Sections were fixed in 4% paraformaldehyde and permeabilized with 0.2% Triton X-100. Antigen retrieval was Heat mediated antigen retrieval using sodium citrate buffer (10mM citrate pH 6.0 + 0.05% Tween-20). Goat anti rabbit IgG (Alexa Fluor® 488, ab150077) was used as the secondary antibody at 1/1000 (2 µg/ml) dilution. DAPI was used as nuclear counterstain. PBS instead of the primary antibody was used as the secondary antibody only control.

      This data was developed using the same antibody clone in a different buffer formulation containing PBS, BSA, glycerol, and sodium azide (ab227643).

    • Flow Cytometry - Anti-KRT6C antibody [SP87] - BSA and Azide free (ab240985)
      Flow Cytometry - Anti-KRT6C antibody [SP87] - BSA and Azide free (ab240985)

      Flow cytometric analysis of A431 (human epidermoid carcinoma cell line) cell line labeling KRT6C with ab227643 at 1/100 dilution (green) compared with a negative control rabbit IgG (blue). 

      This data was developed using the same antibody clone in a different buffer formulation containing PBS, BSA, glycerol, and sodium azide (ab227643).

    • Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-KRT6C antibody [SP87] - BSA and Azide free (ab240985)
      Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-KRT6C antibody [SP87] - BSA and Azide free (ab240985)

      Formalin-fixed, paraffin-embedded human breast tissue stained for KRT6C using ab227643 at 1/200 dilution in immunohistochemical analysis.

      This data was developed using the same antibody clone in a different buffer formulation containing PBS, BSA, glycerol, and sodium azide (ab227643)
    • Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-KRT6C antibody [SP87] - BSA and Azide free (ab240985)
      Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-KRT6C antibody [SP87] - BSA and Azide free (ab240985)

      Formalin-fixed, paraffin-embedded human stomach tissue stained for KRT6C using ab227643 at 1/200 dilution in immunohistochemical analysis.

      This data was developed using the same antibody clone in a different buffer formulation containing PBS, BSA, glycerol, and sodium azide (ab227643)
    • Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-KRT6C antibody [SP87] - BSA and Azide free (ab240985)
      Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-KRT6C antibody [SP87] - BSA and Azide free (ab240985)

      Formalin-fixed, paraffin-embedded human prostate tissue stained for KRT6C using ab227643 at 1/200 dilution in immunohistochemical analysis.

      This data was developed using the same antibody clone in a different buffer formulation containing PBS, BSA, glycerol, and sodium azide (ab227643)
    • Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-KRT6C antibody [SP87] - BSA and Azide free (ab240985)
      Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-KRT6C antibody [SP87] - BSA and Azide free (ab240985)

      Formalin-fixed, paraffin-embedded human lung squamous cell carcinoma tissue stained for KRT6C using ab227643 at 1/200 dilution in immunohistochemical analysis.

      This data was developed using the same antibody clone in a different buffer formulation containing PBS, BSA, glycerol, and sodium azide (ab227643)
    • Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-KRT6C antibody [SP87] - BSA and Azide free (ab240985)
      Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-KRT6C antibody [SP87] - BSA and Azide free (ab240985)

      Formalin-fixed, paraffin-embedded human rectal adenocarcinoma tissue stained for KRT6C using ab227643 at 1/200 dilution in immunohistochemical analysis.

      This data was developed using the same antibody clone in a different buffer formulation containing PBS, BSA, glycerol, and sodium azide (ab227643)
    • Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-KRT6C antibody [SP87] - BSA and Azide free (ab240985)
      Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-KRT6C antibody [SP87] - BSA and Azide free (ab240985)

      Formalin-fixed, paraffin-embedded human bladder tissue stained for KRT6C using ab227643 at 1/200 dilution in immunohistochemical analysis.

      This data was developed using the same antibody clone in a different buffer formulation containing PBS, BSA, glycerol, and sodium azide (ab227643)
    • Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-KRT6C antibody [SP87] - BSA and Azide free (ab240985)
      Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-KRT6C antibody [SP87] - BSA and Azide free (ab240985)

      Formalin-fixed, paraffin-embedded human bladder transitional cell carcinoma tissue stained for KRT6C using ab227643 at 1/200 dilution in immunohistochemical analysis.

      This data was developed using the same antibody clone in a different buffer formulation containing PBS, BSA, glycerol, and sodium azide (ab227643)
    • Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-KRT6C antibody [SP87] - BSA and Azide free (ab240985)
      Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-KRT6C antibody [SP87] - BSA and Azide free (ab240985)

      Formalin-fixed, paraffin-embedded human colon adenocarcinoma tissue stained for KRT6C using ab227643 at 1/200 dilution in immunohistochemical analysis.

      This data was developed using the same antibody clone in a different buffer formulation containing PBS, BSA, glycerol, and sodium azide (ab227643)
    • Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-KRT6C antibody [SP87] - BSA and Azide free (ab240985)
      Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-KRT6C antibody [SP87] - BSA and Azide free (ab240985)

      Formalin-fixed, paraffin-embedded human ovarian adenocarcinoma tissue stained for KRT6C using ab227643 at 1/200 dilution in immunohistochemical analysis.

      This data was developed using the same antibody clone in a different buffer formulation containing PBS, BSA, glycerol, and sodium azide (ab227643)
    • Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-KRT6C antibody [SP87] - BSA and Azide free (ab240985)
      Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-KRT6C antibody [SP87] - BSA and Azide free (ab240985)

      Formalin-fixed, paraffin-embedded human placenta tissue stained for KRT6C using ab227643 at 1/200 dilution in immunohistochemical analysis.

      This data was developed using the same antibody clone in a different buffer formulation containing PBS, BSA, glycerol, and sodium azide (ab227643)
    • Anti-KRT6C antibody [SP87] - BSA and Azide free (ab240985)
      Anti-KRT6C antibody [SP87] - BSA and Azide free (ab240985)

    Protocols

    To our knowledge, customised protocols are not required for this product. Please try the standard protocols listed below and let us know how you get on.

    Click here to view the general protocols

    References (0)

    Publishing research using ab240985? Please let us know so that we can cite the reference in this datasheet.

    ab240985 has not yet been referenced specifically in any publications.

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    Please note: All products are "FOR RESEARCH USE ONLY. NOT FOR USE IN DIAGNOSTIC PROCEDURES"
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