Anti-KRTHA3B antibody (ab154339)
Key features and details
- Rabbit polyclonal to KRTHA3B
- Suitable for: WB, IHC-P, ICC/IF
- Reacts with: Human
- Isotype: IgG
Overview
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Product name
Anti-KRTHA3B antibody -
Description
Rabbit polyclonal to KRTHA3B -
Host species
Rabbit -
Tested applications
Suitable for: WB, IHC-P, ICC/IFmore details -
Species reactivity
Reacts with: Human -
Immunogen
Recombinant fragment, corresponding to a region within amino acids 140-404 of Human KRTHA3B (Uniprot ID: Q14525)
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Positive control
- HeLa cells and whole cell lysate, paraffin-embedded Cal27 xenograft
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General notes
The Life Science industry has been in the grips of a reproducibility crisis for a number of years. Abcam is leading the way in addressing this with our range of recombinant monoclonal antibodies and knockout edited cell lines for gold-standard validation. Please check that this product meets your needs before purchasing.
If you have any questions, special requirements or concerns, please send us an inquiry and/or contact our Support team ahead of purchase. Recommended alternatives for this product can be found below, along with publications, customer reviews and Q&As
Properties
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Form
Liquid -
Storage instructions
Shipped at 4°C. Upon delivery aliquot. Store at -20°C or -80°C. Avoid freeze / thaw cycle. -
Storage buffer
pH: 7.00
Preservative: 0.01% Thimerosal (merthiolate)
Constituents: 79.99% PBS, 20% Glycerol (glycerin, glycerine) -
Concentration information loading...
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Purity
Immunogen affinity purified -
Clonality
Polyclonal -
Isotype
IgG -
Research areas
Associated products
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Compatible Secondaries
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Isotype control
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Positive Controls
Applications
The Abpromise guarantee
Our Abpromise guarantee covers the use of ab154339 in the following tested applications.
The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.
Application | Abreviews | Notes |
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WB |
1/5000 - 1/20000. Predicted molecular weight: 46 kDa.
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IHC-P |
1/100 - 1/1000. Perform heat mediated antigen retrieval with citrate buffer pH 6 before commencing with IHC staining protocol. Alternatively Tris-EDTA buffer (pH8.0) may be used.
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ICC/IF |
1/100 - 1/1000.
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Notes |
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WB
1/5000 - 1/20000. Predicted molecular weight: 46 kDa. |
IHC-P
1/100 - 1/1000. Perform heat mediated antigen retrieval with citrate buffer pH 6 before commencing with IHC staining protocol. Alternatively Tris-EDTA buffer (pH8.0) may be used. |
ICC/IF
1/100 - 1/1000. |
Target
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Sequence similarities
Belongs to the intermediate filament family. - Information by UniProt
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Database links
- Entrez Gene: 3884 Human
- Omim: 602762 Human
- SwissProt: Q14525 Human
- Unigene: 32950 Human
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Alternative names
- HA 3II antibody
- HA3II antibody
- Hair keratin antibody
see all
Images
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Anti-KRTHA3B antibody (ab154339) at 1/10000 dilution + HeLa whole cell lysate at 30 µg
Predicted band size: 46 kDa
10% SDS PAGE -
Immunofluorescence analysis of methanol-fixed HeLa cells labeling KRTHA3B with ab154339 at 1/500 dilution (green) and counterstained with Hoechst 3342 in bottom panel (blue).
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Immunohistochemical analysis of paraffin-embedded Cal27 xenograft, labeling KRTHA3B with ab154339 at 1/500 dilution
Protocols
Datasheets and documents
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SDS download
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Datasheet download
References (0)
ab154339 has not yet been referenced specifically in any publications.