Key features and details
- Rabbit polyclonal to KRTHA3B
- Suitable for: WB, IHC-P, ICC/IF
- Reacts with: Human
- Isotype: IgG
Product nameAnti-KRTHA3B antibody
DescriptionRabbit polyclonal to KRTHA3B
Tested applicationsSuitable for: WB, IHC-P, ICC/IFmore details
Species reactivityReacts with: Human
Recombinant fragment, corresponding to a region within amino acids 140-404 of Human KRTHA3B (Uniprot ID: Q14525)
- HeLa cells and whole cell lysate, paraffin-embedded Cal27 xenograft
Storage instructionsShipped at 4°C. Upon delivery aliquot. Store at -20°C or -80°C. Avoid freeze / thaw cycle.
Storage bufferpH: 7.00
Preservative: 0.01% Thimerosal (merthiolate)
Constituents: PBS, 20% Glycerol
Concentration information loading...
PurityImmunogen affinity purified
Our Abpromise guarantee covers the use of ab154339 in the following tested applications.
The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.
|WB||1/5000 - 1/20000. Predicted molecular weight: 46 kDa.|
|IHC-P||1/100 - 1/1000. Perform heat mediated antigen retrieval with citrate buffer pH 6 before commencing with IHC staining protocol. Alternatively Tris-EDTA buffer (pH8.0) may be used.|
|ICC/IF||1/100 - 1/1000.|
Sequence similaritiesBelongs to the intermediate filament family.
- Information by UniProt
- HA 3II antibody
- HA3II antibody
- Hair keratin antibody
Anti-KRTHA3B antibody (ab154339) at 1/10000 dilution + HeLa whole cell lysate at 30 µg
Predicted band size: 46 kDa
10% SDS PAGE
Immunofluorescence analysis of methanol-fixed HeLa cells labeling KRTHA3B with ab154339 at 1/500 dilution (green) and counterstained with Hoechst 3342 in bottom panel (blue).
Immunohistochemical analysis of paraffin-embedded Cal27 xenograft, labeling KRTHA3B with ab154339 at 1/500 dilution
ab154339 has not yet been referenced specifically in any publications.