Recombinant Anti-KRTHB3 antibody [EPR12333] (ab174272)
![Western blot - Anti-KRTHB3 antibody [EPR12333] (ab174272)](https://www.abcam.com/ps/products/174/ab174272/Images/ab174272-199149-ab174272.jpg "Western blot - Anti-KRTHB3 antibody [EPR12333] (ab174272)")
Key features and details
- Produced recombinantly (animal-free) for high batch-to-batch consistency and long term security of supply
- Rabbit monoclonal [EPR12333] to KRTHB3
- Suitable for: WB
- Reacts with: Mouse, Rat, Human
Overview
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Product name
Anti-KRTHB3 antibody [EPR12333]
See all KRTHB3 primary antibodies -
Description
Rabbit monoclonal [EPR12333] to KRTHB3 -
Host species
Rabbit -
Tested applications
Suitable for: WBmore details
Unsuitable for: Flow Cyt,ICC/IF,IHC-P or IP -
Species reactivity
Reacts with: Mouse, Rat, Human -
Immunogen
Recombinant fragment within Human KRTHB3. The exact sequence is proprietary.
Database link: P78385 -
Positive control
- WB: Human skin lysate. C6 and NIH/3T3 cell lysates.
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General notes
This product is a recombinant monoclonal antibody, which offers several advantages including:
- - High batch-to-batch consistency and reproducibility
- - Improved sensitivity and specificity
- - Long-term security of supply
- - Animal-free production
Our RabMAb® technology is a patented hybridoma-based technology for making rabbit monoclonal antibodies. For details on our patents, please refer to RabMAb® patents.
Reproducibility is key to advancing scientific discovery and accelerating scientists’ next breakthrough.
Abcam is leading the way with our range of recombinant antibodies, knockout-validated antibodies and knockout cell lines, all of which support improved reproducibility.
We are also planning to innovate the way in which we present recommended applications and species on our product datasheets, so that only applications & species that have been tested in our own labs, our suppliers or by selected trusted collaborators are covered by our Abpromise™ guarantee.
In preparation for this, we have started to update the applications & species that this product is Abpromise guaranteed for.
We are also updating the applications & species that this product has been “predicted to work with,” however this information is not covered by our Abpromise guarantee.
Applications & species from publications and Abreviews that have not been tested in our own labs or in those of our suppliers are not covered by the Abpromise guarantee.
Please check that this product meets your needs before purchasing. If you have any questions, special requirements or concerns, please send us an inquiry and/or contact our Support team ahead of purchase. Recommended alternatives for this product can be found below, as well as customer reviews and Q&As.
Properties
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Form
Liquid -
Storage instructions
Shipped at 4°C. Store at +4°C short term (1-2 weeks). Upon delivery aliquot. Store at -20°C long term. Avoid freeze / thaw cycle. -
Storage buffer
pH: 7.20
Preservative: 0.01% Sodium azide
Constituents: 9% PBS, 40% Glycerol (glycerin, glycerine), 0.05% BSA, 50% Tissue culture supernatant -
Concentration information loading... -
Purity
Tissue culture supernatant -
Clonality
Monoclonal -
Clone number
EPR12333 -
Isotype
IgG -
Research areas
Associated products
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Alternative Versions
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Isotype control
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Positive Controls
Applications
Our Abpromise guarantee covers the use of ab174272 in the following tested applications.
The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.
| Application | Abreviews | Notes |
|---|---|---|
| WB | 1/1000 - 1/10000. Detects a band of approximately 54 kDa (predicted molecular weight: 54 kDa). |
Target
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Tissue specificity
Synthesis begins in the cortex 10-15 cell layers above the apex of the dermal papilla and ends abruptly in the middle of the cortex. -
Involvement in disease
Defects in KRT83 are a cause of monilethrix (MLTRX) [MIM:158000]. Monilethrix is an autosomal dominant hair disorder characterized clinically by alopecia and follicular papules. Affected hairs have uniform elliptical nodes of normal thickness and intermittent constrictions, internodes at which the hair easily breaks. Usually only the scalp is involved, but in severe forms, the secondary sexual hair, eyebrows, eyelashes, and nails may also be affected. -
Sequence similarities
Belongs to the intermediate filament family. - Information by UniProt
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Database links
- Entrez Gene: 3889 Human
- Entrez Gene: 406219 Mouse
- Entrez Gene: 300246 Rat
- Omim: 602765 Human
- SwissProt: P78385 Human
- SwissProt: Q6IMF0 Mouse
- Unigene: 720768 Human
- Unigene: 347934 Mouse
see all -
Alternative names
- Hair keratin K2.10 antibody
- Hard keratin, type II, 3 antibody
- HB 3 antibody
see all
Images
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Western blot - Anti-KRTHB3 antibody [EPR12333] (ab174272)All lanes : Anti-KRTHB3 antibody [EPR12333] (ab174272) at 1/1000 dilution
Lane 1 : Human skin lysate
Lane 2 : C6 lysate
Lane 3 : NIH 3T3 lysate
Lysates/proteins at 10 µg per lane.
Predicted band size: 54 kDa -
![Anti-KRTHB3 antibody [EPR12333] (ab174272)](https://www.abcam.com/ps/products/174/ab174272/Images/ab174272-2-benefits-of-recombinant-antibodies.png)
Anti-KRTHB3 antibody [EPR12333] (ab174272)
Datasheets and documents
References (0)
ab174272 has not yet been referenced specifically in any publications.
