Recombinant
Recombinant Anti-KRTHB3 antibody [EPR12333] (ab174272)
- Datasheet
- References
- Protocols
Overview
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Product name
Anti-KRTHB3 antibody [EPR12333]
See all KRTHB3 primary antibodies -
Description
Rabbit monoclonal [EPR12333] to KRTHB3 -
Host species
Rabbit -
Tested applications
Suitable for: WBmore details
Unsuitable for: Flow Cyt,ICC/IF,IHC-P or IP -
Species reactivity
Reacts with: Mouse, Rat, Human -
Immunogen
Recombinant fragment within Human KRTHB3. The exact sequence is proprietary.
Database link: P78385 -
Positive control
- WB: Human skin lysate. C6 and NIH/3T3 cell lysates.
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General notes
Our RabMAb® technology is a patented hybridoma-based technology for making rabbit monoclonal antibodies. For details on our patents, please refer to RabMab® patents.
This product is a recombinant rabbit monoclonal antibody.
Properties
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Form
Liquid -
Storage instructions
Shipped at 4°C. Store at +4°C short term (1-2 weeks). Upon delivery aliquot. Store at -20°C long term. Avoid freeze / thaw cycle. -
Storage buffer
pH: 7.20
Preservative: 0.01% Sodium azide
Constituents: 9% PBS, 40% Glycerol, 0.05% BSA, 50% Tissue culture supernatant -
Purity
Tissue culture supernatant -
Clonality
Monoclonal -
Clone number
EPR12333 -
Isotype
IgG -
Research areas
Associated products
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Alternative Versions
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Isotype control
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Positive Controls
Applications
Our Abpromise guarantee covers the use of ab174272 in the following tested applications.
The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.
| Application | Abreviews | Notes |
|---|---|---|
| WB | 1/1000 - 1/10000. Detects a band of approximately 54 kDa (predicted molecular weight: 54 kDa). |
Application notes
Is unsuitable for Flow Cyt,ICC/IF,IHC-P or IP.
Target
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Tissue specificity
Synthesis begins in the cortex 10-15 cell layers above the apex of the dermal papilla and ends abruptly in the middle of the cortex. -
Involvement in disease
Defects in KRT83 are a cause of monilethrix (MLTRX) [MIM:158000]. Monilethrix is an autosomal dominant hair disorder characterized clinically by alopecia and follicular papules. Affected hairs have uniform elliptical nodes of normal thickness and intermittent constrictions, internodes at which the hair easily breaks. Usually only the scalp is involved, but in severe forms, the secondary sexual hair, eyebrows, eyelashes, and nails may also be affected. -
Sequence similarities
Belongs to the intermediate filament family. - Information by UniProt
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Database links
- Entrez Gene: 3889 Human
- Entrez Gene: 406219 Mouse
- Entrez Gene: 300246 Rat
- Omim: 602765 Human
- SwissProt: P78385 Human
- SwissProt: Q6IMF0 Mouse
- Unigene: 720768 Human
- Unigene: 347934 Mouse
see all -
Alternative names
- Hair keratin K2.10 antibody
- Hard keratin, type II, 3 antibody
- HB 3 antibody
see all
Images
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![Western blot - Anti-KRTHB3 antibody [EPR12333] (ab174272)](https://www.abcam.com/ps/products/174/ab174272/Images/ab174272-199149-ab174272.jpg)
Western blot - Anti-KRTHB3 antibody [EPR12333] (ab174272)All lanes : Anti-KRTHB3 antibody [EPR12333] (ab174272) at 1/1000 dilution
Lane 1 : Human skin lysate
Lane 2 : C6 lysate
Lane 3 : NIH 3T3 lysate
Lysates/proteins at 10 µg per lane.
Predicted band size: 54 kDa
Protocols
Datasheets and documents
References
ab174272 has not yet been referenced specifically in any publications.
