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Signal Transduction Protein Phosphorylation Tyrosine Kinases Other
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RabMAb

Anti-KSR1 (phospho S404) antibody [EP2358Y] (ab76248)

  • Datasheet
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Western blot - Anti-KSR1 (phospho S404) antibody [EP2358Y] (ab76248)
  • Immunocytochemistry/ Immunofluorescence - KSR1 (phospho S404) antibody [EP2358Y] (ab76248)

Key features and details

  • Rabbit monoclonal [EP2358Y] to KSR1 (phospho S404)
  • Suitable for: ICC/IF, WB
  • Reacts with: Human
  • Isotype: IgG

You may also be interested in

Protein
Product image
Recombinant human KSR1 protein (ab190396)
Secondary
Product image
Goat Anti-Rabbit IgG H&L (HRP) (ab205718)

View more associated products

Overview

  • Product name

    Anti-KSR1 (phospho S404) antibody [EP2358Y]
    See all KSR1 primary antibodies
  • Description

    Rabbit monoclonal [EP2358Y] to KSR1 (phospho S404)
  • Host species

    Rabbit
  • Tested applications

    Suitable for: ICC/IF, WBmore details
    Unsuitable for: Flow Cyt,IHC-P or IP
  • Species reactivity

    Reacts with: Human
  • Immunogen

    Synthetic phospho-peptide corresponding to residues surrounding Serine 404 of human KSR1

  • Positive control

    • WB: HeLa cell lysates, untreated or treated with Calyculin A IF: Sh-SY-5Y cells
  • General notes

    Mouse, Rat: We have preliminary internal testing data to indicate this antibody may not react with these species. Please contact us for more information.

    Our RabMAb® technology is a patented hybridoma-based technology for making rabbit monoclonal antibodies. For details on our patents, please refer to RabMAb® patents.

    Reproducibility is key to advancing scientific discovery and accelerating scientists’ next breakthrough.

    Abcam is leading the way with our range of recombinant antibodies, knockout-validated antibodies and knockout cell lines, all of which support improved reproducibility.

    We are also planning to innovate the way in which we present recommended applications and species on our product datasheets, so that only applications & species that have been tested in our own labs, our suppliers or by selected trusted collaborators are covered by our Abpromise™ guarantee.

    In preparation for this, we have started to update the applications & species that this product is Abpromise guaranteed for.

    We are also updating the applications & species that this product has been “predicted to work with,” however this information is not covered by our Abpromise guarantee.

    Applications & species from publications and Abreviews that have not been tested in our own labs or in those of our suppliers are not covered by the Abpromise guarantee.

    Please check that this product meets your needs before purchasing. If you have any questions, special requirements or concerns, please send us an inquiry and/or contact our Support team ahead of purchase. Recommended alternatives for this product can be found below, as well as customer reviews and Q&As.

Properties

  • Form

    Liquid
  • Storage instructions

    Shipped at 4°C. Store at -20°C. Stable for 12 months at -20°C.
  • Storage buffer

    pH: 7.20
    Preservative: 0.05% Sodium azide
    Constituents: 0.1% BSA, 40% Glycerol (glycerin, glycerine), 9.85% Tris glycine, 50% Tissue culture supernatant
  • Concentration information loading...
  • Purity

    Tissue culture supernatant
  • Clonality

    Monoclonal
  • Clone number

    EP2358Y
  • Isotype

    IgG
  • Research areas

    • Signal Transduction
    • Protein Phosphorylation
    • Tyrosine Kinases
    • Other
    • Signal Transduction
    • Protein Phosphorylation
    • Ser / Thr Kinases
    • MAPK Pathway
    • Signal Transduction
    • Protein Phosphorylation
    • Ser / Thr Kinases
    • Other Kinases

Associated products

  • Isotype control

    • Rabbit IgG, monoclonal [EPR25A] - Isotype Control (ab172730)
  • Positive Controls

    • HeLa membrane extract lysate (ab29547)
  • Recombinant Protein

    • Recombinant human KSR1 protein (ab190396)

Applications

Our Abpromise guarantee covers the use of ab76248 in the following tested applications.

The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.

Application Abreviews Notes
ICC/IF 1/100 - 1/250.
WB 1/1000 - 1/10000. Predicted molecular weight: 102 kDa.
  • Application notes
    Is unsuitable for Flow Cyt,IHC-P or IP.
  • Target

    • Function

      Location-regulated scaffolding protein connecting MEK to RAF. Promotes MEK and RAF phosphorylation and activity through assembly of an activated signaling complex. By itself, it has no demonstrated kinase activity.
    • Sequence similarities

      Belongs to the protein kinase superfamily. TKL Ser/Thr protein kinase family.
      Contains 1 phorbol-ester/DAG-type zinc finger.
      Contains 1 protein kinase domain.
    • Post-translational
      modifications

      Phosphorylated on Ser-309 and, to a higher extent, on Ser-404 by MARK3. Dephosphorylated on Ser-404 by PPP2CA. In resting cells, phosphorylated KSR1 is cytoplasmic and in stimulated cells, dephosphorylated KSR1 is membrane-associated.
    • Cellular localization

      Cytoplasm. Membrane. In unstimulated cells, where the phosphorylated form is bound to a 14-3-3 protein, sequestration in the cytoplasm occurs. Following growth factor treatment, the protein is free for membrane translocation, and it moves from the cytoplasm to the cell periphery.
    • Target information above from: UniProt accession Q8IVT5 The UniProt Consortium
      The Universal Protein Resource (UniProt) in 2010
      Nucleic Acids Res. 38:D142-D148 (2010) .

      Information by UniProt
    • Database links

      • Entrez Gene: 8844 Human
      • Omim: 601132 Human
      • SwissProt: Q8IVT5 Human
      • Unigene: 133534 Human
      • Alternative names

        • BKSR 1 antibody
        • BKSR1 antibody
        • dKsr antibody
        • EK 31 antibody
        • EK31 antibody
        • hb antibody
        • Kinase suppressor of Ras 1 antibody
        • Kinase suppressor of ras antibody
        • KSR 1 antibody
        • KSR antibody
        • KSR1 antibody
        • KSR1_HUMAN antibody
        • Positive Ras signaling mediator family member (86.4 kD) antibody
        • Positive Ras signaling mediator family member antibody
        • RSU 2 antibody
        • RSU2 antibody
        • SR 31 antibody
        • SR31 antibody
        • Suppressor of activated let 60 Ras SUR3 antibody
        • Suppressor of Ras1 31 antibody
        see all

      Images

      • Western blot - Anti-KSR1 (phospho S404) antibody [EP2358Y] (ab76248)
        Western blot - Anti-KSR1 (phospho S404) antibody [EP2358Y] (ab76248)
        All lanes : Anti-KSR1 (phospho S404) antibody [EP2358Y] (ab76248) at 1/2000 dilution

        Lane 1 : HeLa cell lysate (untreated)
        Lane 2 : HeLa cell lysate, treated with Calyculin A

        Lysates/proteins at 10 µg per lane.

        Secondary
        All lanes : goat anti-rabbit HRP labelled IgG at 1/1000 dilution

        Predicted band size: 102 kDa
        Observed band size: 110 kDa
        why is the actual band size different from the predicted?

      • Immunocytochemistry/ Immunofluorescence - KSR1 (phospho S404) antibody [EP2358Y] (ab76248)
        Immunocytochemistry/ Immunofluorescence - KSR1 (phospho S404) antibody [EP2358Y] (ab76248)
        Immunofluorescent staining of Sh-SY-5Y cells, using ab76248, at 1/100 dilution.

      Protocols

      • Western blot protocols
      • Immunocytochemistry & immunofluorescence protocols

      Click here to view the general protocols

      Datasheets and documents

      • Datasheet
    • References (0)

      Publishing research using ab76248? Please let us know so that we can cite the reference in this datasheet.

      ab76248 has not yet been referenced specifically in any publications.

      Customer reviews and Q&As

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      Please use the links above to contact us or submit feedback about this product.

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