Anti-KSR1 (phospho S404) antibody [EP2358Y] (ab76248)
![Western blot - Anti-KSR1 (phospho S404) antibody [EP2358Y] (ab76248)](https://www.abcam.com/ps/products/76/ab76248/Images/ab76248.JPG "Western blot - Anti-KSR1 (phospho S404) antibody [EP2358Y] (ab76248)")
Key features and details
- Rabbit monoclonal [EP2358Y] to KSR1 (phospho S404)
- Suitable for: ICC/IF, WB
- Reacts with: Human
- Isotype: IgG
Overview
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Product name
Anti-KSR1 (phospho S404) antibody [EP2358Y]
See all KSR1 primary antibodies -
Description
Rabbit monoclonal [EP2358Y] to KSR1 (phospho S404) -
Host species
Rabbit -
Tested applications
Suitable for: ICC/IF, WBmore details
Unsuitable for: Flow Cyt,IHC-P or IP -
Species reactivity
Reacts with: Human -
Immunogen
Synthetic phospho-peptide corresponding to residues surrounding Serine 404 of human KSR1
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Positive control
- WB: HeLa cell lysates, untreated or treated with Calyculin A IF: Sh-SY-5Y cells
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General notes
Mouse, Rat: We have preliminary internal testing data to indicate this antibody may not react with these species. Please contact us for more information.
Our RabMAb® technology is a patented hybridoma-based technology for making rabbit monoclonal antibodies. For details on our patents, please refer to RabMAb® patents.
Reproducibility is key to advancing scientific discovery and accelerating scientists’ next breakthrough.
Abcam is leading the way with our range of recombinant antibodies, knockout-validated antibodies and knockout cell lines, all of which support improved reproducibility.
We are also planning to innovate the way in which we present recommended applications and species on our product datasheets, so that only applications & species that have been tested in our own labs, our suppliers or by selected trusted collaborators are covered by our Abpromise™ guarantee.
In preparation for this, we have started to update the applications & species that this product is Abpromise guaranteed for.
We are also updating the applications & species that this product has been “predicted to work with,” however this information is not covered by our Abpromise guarantee.
Applications & species from publications and Abreviews that have not been tested in our own labs or in those of our suppliers are not covered by the Abpromise guarantee.
Please check that this product meets your needs before purchasing. If you have any questions, special requirements or concerns, please send us an inquiry and/or contact our Support team ahead of purchase. Recommended alternatives for this product can be found below, as well as customer reviews and Q&As.
Properties
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Form
Liquid -
Storage instructions
Shipped at 4°C. Store at -20°C. Stable for 12 months at -20°C. -
Storage buffer
pH: 7.20
Preservative: 0.05% Sodium azide
Constituents: 0.1% BSA, 40% Glycerol (glycerin, glycerine), 9.85% Tris glycine, 50% Tissue culture supernatant -
Concentration information loading... -
Purity
Tissue culture supernatant -
Clonality
Monoclonal -
Clone number
EP2358Y -
Isotype
IgG -
Research areas
Associated products
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Isotype control
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Positive Controls
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Recombinant Protein
Applications
Our Abpromise guarantee covers the use of ab76248 in the following tested applications.
The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.
| Application | Abreviews | Notes |
|---|---|---|
| ICC/IF | 1/100 - 1/250. | |
| WB | 1/1000 - 1/10000. Predicted molecular weight: 102 kDa. |
Target
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Function
Location-regulated scaffolding protein connecting MEK to RAF. Promotes MEK and RAF phosphorylation and activity through assembly of an activated signaling complex. By itself, it has no demonstrated kinase activity. -
Sequence similarities
Belongs to the protein kinase superfamily. TKL Ser/Thr protein kinase family.
Contains 1 phorbol-ester/DAG-type zinc finger.
Contains 1 protein kinase domain. -
Post-translational
modificationsPhosphorylated on Ser-309 and, to a higher extent, on Ser-404 by MARK3. Dephosphorylated on Ser-404 by PPP2CA. In resting cells, phosphorylated KSR1 is cytoplasmic and in stimulated cells, dephosphorylated KSR1 is membrane-associated. -
Cellular localization
Cytoplasm. Membrane. In unstimulated cells, where the phosphorylated form is bound to a 14-3-3 protein, sequestration in the cytoplasm occurs. Following growth factor treatment, the protein is free for membrane translocation, and it moves from the cytoplasm to the cell periphery. - Information by UniProt
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Database links
- Entrez Gene: 8844 Human
- Omim: 601132 Human
- SwissProt: Q8IVT5 Human
- Unigene: 133534 Human
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Alternative names
- BKSR 1 antibody
- BKSR1 antibody
- dKsr antibody
see all
Images
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Western blot - Anti-KSR1 (phospho S404) antibody [EP2358Y] (ab76248)All lanes : Anti-KSR1 (phospho S404) antibody [EP2358Y] (ab76248) at 1/2000 dilution
Lane 1 : HeLa cell lysate (untreated)
Lane 2 : HeLa cell lysate, treated with Calyculin A
Lysates/proteins at 10 µg per lane.
Secondary
All lanes : goat anti-rabbit HRP labelled IgG at 1/1000 dilution
Predicted band size: 102 kDa
Observed band size: 110 kDa why is the actual band size different from the predicted? -
![Immunocytochemistry/ Immunofluorescence - KSR1 (phospho S404) antibody [EP2358Y] (ab76248)](https://www.abcam.com/ps/products/76/ab76248/Images/ab76248 IF.JPG)
Immunocytochemistry/ Immunofluorescence - KSR1 (phospho S404) antibody [EP2358Y] (ab76248)Immunofluorescent staining of Sh-SY-5Y cells, using ab76248, at 1/100 dilution.
Protocols
Datasheets and documents
References (0)
ab76248 has not yet been referenced specifically in any publications.
