Anti-KSR1 (phospho S404) antibody [EP2358Y] (ab76248)
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Overview
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Product name
Anti-KSR1 (phospho S404) antibody [EP2358Y]
See all KSR1 primary antibodies -
Description
Rabbit monoclonal [EP2358Y] to KSR1 (phospho S404) -
Host species
Rabbit -
Tested applications
Suitable for: ICC/IF, WBmore details
Unsuitable for: Flow Cyt,IHC-P or IP -
Species reactivity
Reacts with: Human -
Immunogen
Synthetic phospho-peptide corresponding to residues surrounding Serine 404 of human KSR1
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Positive control
- WB: HeLa cell lysates, untreated or treated with Calyculin A IF: Sh-SY-5Y cells
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General notes
Mouse, Rat: We have preliminary internal testing data to indicate this antibody may not react with these species. Please contact us for more information.
Our RabMAb® technology is a patented hybridoma-based technology for making rabbit monoclonal antibodies. For details on our patents, please refer to RabMab® patents.
Properties
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Form
Liquid -
Storage instructions
Shipped at 4°C. Store at -20°C. Stable for 12 months at -20°C. -
Storage buffer
pH: 7.20
Preservative: 0.05% Sodium azide
Constituents: 0.1% BSA, 40% Glycerol, 9.85% Tris glycine, 50% Tissue culture supernatant -
Purity
Tissue culture supernatant -
Clonality
Monoclonal -
Clone number
EP2358Y -
Isotype
IgG -
Research areas
Associated products
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Isotype control
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Positive Controls
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Recombinant Protein
Applications
Our Abpromise guarantee covers the use of ab76248 in the following tested applications.
The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.
| Application | Abreviews | Notes |
|---|---|---|
| ICC/IF | 1/100 - 1/250. | |
| WB | 1/1000 - 1/10000. Predicted molecular weight: 102 kDa. |
Target
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Function
Location-regulated scaffolding protein connecting MEK to RAF. Promotes MEK and RAF phosphorylation and activity through assembly of an activated signaling complex. By itself, it has no demonstrated kinase activity. -
Sequence similarities
Belongs to the protein kinase superfamily. TKL Ser/Thr protein kinase family.
Contains 1 phorbol-ester/DAG-type zinc finger.
Contains 1 protein kinase domain. -
Post-translational
modificationsPhosphorylated on Ser-309 and, to a higher extent, on Ser-404 by MARK3. Dephosphorylated on Ser-404 by PPP2CA. In resting cells, phosphorylated KSR1 is cytoplasmic and in stimulated cells, dephosphorylated KSR1 is membrane-associated. -
Cellular localization
Cytoplasm. Membrane. In unstimulated cells, where the phosphorylated form is bound to a 14-3-3 protein, sequestration in the cytoplasm occurs. Following growth factor treatment, the protein is free for membrane translocation, and it moves from the cytoplasm to the cell periphery. - Information by UniProt
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Database links
- Entrez Gene: 8844 Human
- Omim: 601132 Human
- SwissProt: Q8IVT5 Human
- Unigene: 133534 Human
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Alternative names
- BKSR 1 antibody
- BKSR1 antibody
- dKsr antibody
see all
Images
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![Western blot - Anti-KSR1 (phospho S404) antibody [EP2358Y] (ab76248)](https://www.abcam.com/ps/products/76/ab76248/Images/ab76248.JPG)
Western blot - Anti-KSR1 (phospho S404) antibody [EP2358Y] (ab76248)All lanes : Anti-KSR1 (phospho S404) antibody [EP2358Y] (ab76248) at 1/2000 dilution
Lane 1 : HeLa cell lysate (untreated)
Lane 2 : HeLa cell lysate, treated with Calyculin A
Lysates/proteins at 10 µg per lane.
Secondary
All lanes : goat anti-rabbit HRP labelled IgG at 1/1000 dilution
Predicted band size: 102 kDa
Observed band size: 110 kDa why is the actual band size different from the predicted? -
![Immunocytochemistry/ Immunofluorescence - KSR1 (phospho S404) antibody [EP2358Y] (ab76248)](https://www.abcam.com/ps/products/76/ab76248/Images/ab76248 IF.JPG)
Immunocytochemistry/ Immunofluorescence - KSR1 (phospho S404) antibody [EP2358Y] (ab76248)Immunofluorescent staining of Sh-SY-5Y cells, using ab76248, at 1/100 dilution.
Datasheets and documents
References
ab76248 has not yet been referenced specifically in any publications.
