Product nameAnti-KSR1 (phospho S404) antibody [EP2358Y]
See all KSR1 primary antibodies
DescriptionRabbit monoclonal [EP2358Y] to KSR1 (phospho S404)
Tested applicationsSuitable for: ICC/IF, WBmore details
Unsuitable for: Flow Cyt,IHC-P or IP
Species reactivityReacts with: Human
Synthetic phospho-peptide corresponding to residues surrounding Serine 404 of human KSR1
- WB: HeLa cell lysates, untreated or treated with Calyculin A IF: Sh-SY-5Y cells
Mouse, Rat: We have preliminary internal testing data to indicate this antibody may not react with these species. Please contact us for more information.
Our RabMAb® technology is a patented hybridoma-based technology for making rabbit monoclonal antibodies. For details on our patents, please refer to RabMab® patents.
Storage instructionsShipped at 4°C. Store at -20°C. Stable for 12 months at -20°C.
Storage bufferpH: 7.20
Preservative: 0.05% Sodium azide
Constituents: 0.1% BSA, 40% Glycerol, 9.85% Tris glycine, 50% Tissue culture supernatant
PurityTissue culture supernatant
Our Abpromise guarantee covers the use of ab76248 in the following tested applications.
The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.
|ICC/IF||1/100 - 1/250.|
|WB||1/1000 - 1/10000. Predicted molecular weight: 102 kDa.|
FunctionLocation-regulated scaffolding protein connecting MEK to RAF. Promotes MEK and RAF phosphorylation and activity through assembly of an activated signaling complex. By itself, it has no demonstrated kinase activity.
Sequence similaritiesBelongs to the protein kinase superfamily. TKL Ser/Thr protein kinase family.
Contains 1 phorbol-ester/DAG-type zinc finger.
Contains 1 protein kinase domain.
modificationsPhosphorylated on Ser-309 and, to a higher extent, on Ser-404 by MARK3. Dephosphorylated on Ser-404 by PPP2CA. In resting cells, phosphorylated KSR1 is cytoplasmic and in stimulated cells, dephosphorylated KSR1 is membrane-associated.
Cellular localizationCytoplasm. Membrane. In unstimulated cells, where the phosphorylated form is bound to a 14-3-3 protein, sequestration in the cytoplasm occurs. Following growth factor treatment, the protein is free for membrane translocation, and it moves from the cytoplasm to the cell periphery.
- Information by UniProt
- BKSR 1 antibody
- BKSR1 antibody
- dKsr antibody
All lanes : Anti-KSR1 (phospho S404) antibody [EP2358Y] (ab76248) at 1/2000 dilution
Lane 1 : HeLa cell lysate (untreated)
Lane 2 : HeLa cell lysate, treated with Calyculin A
Lysates/proteins at 10 µg per lane.
All lanes : goat anti-rabbit HRP labelled IgG at 1/1000 dilution
Predicted band size: 102 kDa
Observed band size: 110 kDa why is the actual band size different from the predicted?
Immunofluorescent staining of Sh-SY-5Y cells, using ab76248, at 1/100 dilution.
ab76248 has not yet been referenced specifically in any publications.