RabMAb

Anti-KSR1 (phospho S404) antibody [EP2358Y] (ab76248)

Submit an Abreview Submit a Question

Overview

  • Product name
    Anti-KSR1 (phospho S404) antibody [EP2358Y]
    See all KSR1 primary antibodies
  • Description
    Rabbit monoclonal [EP2358Y] to KSR1 (phospho S404)
  • Host species
    Rabbit
  • Tested applications
    Suitable for: ICC/IF, WBmore details
    Unsuitable for: Flow Cyt,IHC-P or IP
  • Species reactivity
    Reacts with: Human
  • Immunogen

    Synthetic phospho-peptide corresponding to residues surrounding Serine 404 of human KSR1

  • Positive control
    • WB: HeLa cell lysates, untreated or treated with Calyculin A IF: Sh-SY-5Y cells
  • General notes

    Mouse, Rat: We have preliminary internal testing data to indicate this antibody may not react with these species. Please contact us for more information.

    Our RabMAb® technology is a patented hybridoma-based technology for making rabbit monoclonal antibodies. For details on our patents, please refer to RabMab® patents

Properties

Associated products

Applications

Our Abpromise guarantee covers the use of ab76248 in the following tested applications.

The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.

Application Abreviews Notes
ICC/IF 1/100 - 1/250.
WB 1/1000 - 1/10000. Predicted molecular weight: 102 kDa.
  • Application notes
    Is unsuitable for Flow Cyt,IHC-P or IP.

    • Target

    • Function
      Location-regulated scaffolding protein connecting MEK to RAF. Promotes MEK and RAF phosphorylation and activity through assembly of an activated signaling complex. By itself, it has no demonstrated kinase activity.
    • Sequence similarities
      Belongs to the protein kinase superfamily. TKL Ser/Thr protein kinase family.
      Contains 1 phorbol-ester/DAG-type zinc finger.
      Contains 1 protein kinase domain.
    • Post-translational
      modifications
      Phosphorylated on Ser-309 and, to a higher extent, on Ser-404 by MARK3. Dephosphorylated on Ser-404 by PPP2CA. In resting cells, phosphorylated KSR1 is cytoplasmic and in stimulated cells, dephosphorylated KSR1 is membrane-associated.
    • Cellular localization
      Cytoplasm. Membrane. In unstimulated cells, where the phosphorylated form is bound to a 14-3-3 protein, sequestration in the cytoplasm occurs. Following growth factor treatment, the protein is free for membrane translocation, and it moves from the cytoplasm to the cell periphery.
    • Information by UniProt
    • Database links
      • Alternative names
        • BKSR 1 antibody
        • BKSR1 antibody
        • dKsr antibody
        • EK 31 antibody
        • EK31 antibody
        • hb antibody
        • Kinase suppressor of Ras 1 antibody
        • Kinase suppressor of ras antibody
        • KSR 1 antibody
        • KSR antibody
        • KSR1 antibody
        • KSR1_HUMAN antibody
        • Positive Ras signaling mediator family member (86.4 kD) antibody
        • Positive Ras signaling mediator family member antibody
        • RSU 2 antibody
        • RSU2 antibody
        • SR 31 antibody
        • SR31 antibody
        • Suppressor of activated let 60 Ras SUR3 antibody
        • Suppressor of Ras1 31 antibody
        see all

      Images

      • Western blot - Anti-KSR1 (phospho S404) antibody [EP2358Y] (ab76248)
        Western blot - Anti-KSR1 (phospho S404) antibody [EP2358Y] (ab76248)
        All lanes : Anti-KSR1 (phospho S404) antibody [EP2358Y] (ab76248) at 1/2000 dilution

        Lane 1 : HeLa cell lysate (untreated)
        Lane 2 : HeLa cell lysate, treated with Calyculin A

        Lysates/proteins at 10 µg per lane.

        Secondary
        All lanes : goat anti-rabbit HRP labelled IgG at 1/1000 dilution

        Predicted band size: 102 kDa
        Observed band size: 110 kDa (why is the actual band size different from the predicted?)

      • Immunocytochemistry/ Immunofluorescence - KSR1 (phospho S404) antibody [EP2358Y] (ab76248)
        Immunocytochemistry/ Immunofluorescence - KSR1 (phospho S404) antibody [EP2358Y] (ab76248)
        Immunofluorescent staining of Sh-SY-5Y cells, using ab76248, at 1/100 dilution.

      References

      ab76248 has not yet been referenced specifically in any publications.

      Publishing research using ab76248? Please let us know so that we can cite the reference in this datasheet.

      Customer reviews and Q&As

      Submit a review Submit a question

      There are currently no Customer reviews or Questions for ab76248.
      Please use the links above to contact us or submit feedback about this product.

      Please note: All products are "FOR RESEARCH USE ONLY AND ARE NOT INTENDED FOR DIAGNOSTIC OR THERAPEUTIC USE"

      Sign up