Product nameAnti-KSR2 antibody
See all KSR2 primary antibodies
DescriptionRabbit polyclonal to KSR2
SpecificityDetects endogenous levels of total KSR2 protein.
Tested applicationsSuitable for: WB, ELISA, ICC/IF, IPmore details
Species reactivityReacts with: Human
Predicted to work with: Mouse
Synthetic peptide from an internal region of human KSR2
- WB: 293 cell extract ICC/IF: HeLa cells
Storage instructionsShipped at 4°C. Store at -20°C. Stable for 12 months at -20°C.
Storage bufferpH: 7.40
Preservative: 0.02% Sodium azide
Constituents: 0.87% Sodium chloride, 50% Glycerol, PBS
Without Mg2+ and Ca2+
Concentration information loading...
PurityImmunogen affinity purified
Purification notesab72753 was Affinity purified from rabbit antiserum by affinity chromatography using epitope specific immunogen.
Our Abpromise guarantee covers the use of ab72753 in the following tested applications.
The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.
|WB||1/500 - 1/1000. Detects a band of approximately 108 kDa (predicted molecular weight: 108 kDa).|
|ICC/IF||1/500 - 1/1000.|
|IP||Use a concentration of 5 µg/ml.|
FunctionLocation-regulated scaffold connecting MEK to RAF. Blocks MAP3K8 kinase activity and MAP3K8-mediated signaling. Acts as a negative regulator of MAP3K3-mediated activation of ERK, JNK and NF-kappa-B pathways, inhibiting MAP3K3-mediated interleukin-8 production.
Tissue specificityMainly expressed in brain and kidney.
Sequence similaritiesBelongs to the protein kinase superfamily. TKL Ser/Thr protein kinase family.
Contains 1 phorbol-ester/DAG-type zinc finger.
Contains 1 protein kinase domain.
DomainThe protein kinase domain is predicted to be catalytically inactive.
modificationsPhosphorylated on Ser-474 by MARK3.
Cellular localizationCytoplasm. Membrane.
- Information by UniProt
- FLJ25965 antibody
- hKSR 2 antibody
- hKSR2 antibody
All lanes : Anti-KSR2 antibody (ab72753) at 1/500 dilution
Lane 1 : 293 cell extract
Lane 2 : 293 cell extract with immunising peptide at 10 µg
Lysates/proteins at 10 µg per lane.
Predicted band size: 108 kDa
Observed band size: 108 kDa
Additional bands at: 55 kDa. We are unsure as to the identity of these extra bands.
Immunofluorescence analysis of KSR2 in HeLa cells, using ab72753 at 1/500 dilution. Right panel was also treated with immunising peptide.
KSR2 was immunoprecipitated using 0.5mg Hek293 whole cell extract, 5µg of Rabbit polyclonal to KSR2 and 50µl of protein G magnetic beads (+). No antibody was added to the control (-).
The antibody was incubated under agitation with Protein G beads for 10min, Hek293 whole cell extract lysate diluted in RIPA buffer was added to each sample and incubated for a further 10min under agitation.
Proteins were eluted by addition of 40µl SDS loading buffer and incubated for 10min at 70oC; 10µl of each sample was separated on a SDS PAGE gel, transferred to a nitrocellulose membrane, blocked with 5% BSA and probed with ab72753.
Secondary: Mouse monoclonal [SB62a] Secondary Antibody to Rabbit IgG light chain (HRP) (ab99697).
Band: 108kDa; KSR2