Anti-Ku70 antibody [2F7F5] (ab202022)
Key features and details
- Mouse monoclonal [2F7F5] to Ku70
- Suitable for: WB, IHC-P, Flow Cyt
- Reacts with: Rat, Human
- Isotype: IgG1
Overview
-
Product name
Anti-Ku70 antibody [2F7F5]
See all Ku70 primary antibodies -
Description
Mouse monoclonal [2F7F5] to Ku70 -
Host species
Mouse -
Tested applications
Suitable for: WB, IHC-P, Flow Cytmore details -
Species reactivity
Reacts with: Rat, Human
Predicted to work with: Mouse -
Immunogen
Recombinant fragment corresponding to Human Ku70 aa 1-250. Expressed in E. Coli.
Database link: P12956 -
Positive control
- Human Ku70 (aa: 6-214) recombinant protein; Ku70 (aa: 6-214)-hIgGFc transfected HEK293 cell lysate; HeLa, PC-2, A549, A431, HepG2 and K562 cell lysates; A431 cells; Human endometrial cancer and cervical cancer tissues
-
General notes
The Life Science industry has been in the grips of a reproducibility crisis for a number of years. Abcam is leading the way in addressing this with our range of recombinant monoclonal antibodies and knockout edited cell lines for gold-standard validation. Please check that this product meets your needs before purchasing.
If you have any questions, special requirements or concerns, please send us an inquiry and/or contact our Support team ahead of purchase. Recommended alternatives for this product can be found below, along with publications, customer reviews and Q&As
Properties
-
Form
Liquid -
Storage instructions
Shipped at 4°C. Store at +4°C short term (1-2 weeks). Upon delivery aliquot. Store at -20°C long term. Avoid freeze / thaw cycle. -
Storage buffer
Preservative: 0.05% Sodium azide
Constituent: 99% PBS -
Concentration information loading...
-
Purity
Protein G purified -
Purification notes
Purified from tissue culture supernatant. -
Clonality
Monoclonal -
Clone number
2F7F5 -
Isotype
IgG1 -
Research areas
Associated products
-
Compatible Secondaries
-
Isotype control
-
Positive Controls
-
Recombinant Protein
-
Related Products
Applications
The Abpromise guarantee
Our Abpromise guarantee covers the use of ab202022 in the following tested applications.
The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.
Application | Abreviews | Notes |
---|---|---|
WB |
1/500 - 1/2000. Predicted molecular weight: 70 kDa.
|
|
IHC-P |
1/200 - 1/1000.
|
|
Flow Cyt |
1/200 - 1/400.
ab170190 - Mouse monoclonal IgG1, is suitable for use as an isotype control with this antibody. |
Notes |
---|
WB
1/500 - 1/2000. Predicted molecular weight: 70 kDa. |
IHC-P
1/200 - 1/1000. |
Flow Cyt
1/200 - 1/400. ab170190 - Mouse monoclonal IgG1, is suitable for use as an isotype control with this antibody. |
Target
-
Function
Single stranded DNA-dependent ATP-dependent helicase. Has a role in chromosome translocation. The DNA helicase II complex binds preferentially to fork-like ends of double-stranded DNA in a cell cycle-dependent manner. It works in the 3'-5' direction. Binding to DNA may be mediated by XRCC6. Involved in DNA non-homologous end joining (NHEJ) required for double-strand break repair and V(D)J recombination. The XRCC5/6 dimer acts as regulatory subunit of the DNA-dependent protein kinase complex DNA-PK by increasing the affinity of the catalytic subunit PRKDC to DNA by 100-fold. The XRCC5/6 dimer is probably involved in stabilizing broken DNA ends and bringing them together. The assembly of the DNA-PK complex to DNA ends is required for the NHEJ ligation step. Required for osteocalcin gene expression. Probably also acts as a 5'-deoxyribose-5-phosphate lyase (5'-dRP lyase), by catalyzing the beta-elimination of the 5' deoxyribose-5-phosphate at an abasic site near double-strand breaks. 5'-dRP lyase activity allows to 'clean' the termini of abasic sites, a class of nucleotide damage commonly associated with strand breaks, before such broken ends can be joined. The XRCC5/6 dimer together with APEX1 acts as a negative regulator of transcription. -
Sequence similarities
Belongs to the ku70 family.
Contains 1 Ku domain.
Contains 1 SAP domain. -
Developmental stage
Expression does not increase during promyelocyte differentiation. -
Post-translational
modificationsPhosphorylation by PRKDC may enhance helicase activity. Phosphorylation of Ser-51 does not affect DNA repair. -
Cellular localization
Nucleus. Chromosome. - Information by UniProt
-
Database links
- Entrez Gene: 2547 Human
- Entrez Gene: 14375 Mouse
- Entrez Gene: 25019 Rat
- Omim: 152690 Human
- SwissProt: P12956 Human
- SwissProt: P23475 Mouse
- Unigene: 292493 Human
- Unigene: 288809 Mouse
-
Alternative names
- 5''-deoxyribose-5-phosphate lyase Ku70 antibody
- 5''-dRP lyase Ku70 antibody
- 70 kDa subunit of Ku antigen antibody
see all
Images
-
Anti-Ku70 antibody [2F7F5] (ab202022) at 1/500 dilution + Ku70 (aa: 6-214) recombinant protein. at 1 µg
Predicted band size: 70 kDaExpected MW is 49.7 kDa
-
All lanes : Anti-Ku70 antibody [2F7F5] (ab202022) at 1/500 dilution
Lane 1 : Non transfected HEK293 cell lysate.
Lane 2 : Ku70 (aa: 6-214)-hIgGFc transfected HEK293 cell lysate.
Lysates/proteins at 1 µg per lane.
Predicted band size: 70 kDa -
All lanes : Anti-Ku70 antibody [2F7F5] (ab202022) at 1/500 dilution
Lane 1 : HeLa cell lysate
Lane 2 : PC-2 cell lysate
Lane 3 : A549 cell lysate
Lane 4 : A431 cell lysate
Lane 5 : HepG2 cell lysate
Lane 6 : K562 cell lysate
Lysates/proteins at 1 µg per lane.
Predicted band size: 70 kDa -
Immunohistochemical analysis of paraffin embedded Human endometrial cancer tissues labeling Ku70 using ab202022 at 1/200 dilution, followed by DAB staining.
-
Immunohistochemical analysis of paraffin embedded Human cervical cancer tissues labeling Ku70 using ab202022 at 1/200 dilution, followed by DAB staining.
-
Flow cytometric analysis of A431 cells labeling Ku70 using ab202022 (green) at 1/200 dilution, and negative control (red).
Protocols
Datasheets and documents
-
Datasheet download
References (2)
ab202022 has been referenced in 2 publications.
- Eberle J et al. A Fibrinogen Alpha Fragment Mitigates Chemotherapy-Induced MLL Rearrangements. Front Oncol 11:689063 (2021). PubMed: 34222016
- Berti M et al. Sequential role of RAD51 paralog complexes in replication fork remodeling and restart. Nat Commun 11:3531 (2020). PubMed: 32669601