Overview

  • Product name

    Anti-Ku70 antibody [7A9E7]
    See all Ku70 primary antibodies
  • Description

    Mouse monoclonal [7A9E7] to Ku70
  • Host species

    Mouse
  • Tested applications

    Suitable for: Flow Cyt, WBmore details
  • Species reactivity

    Reacts with: Human
    Predicted to work with: Mouse
  • Immunogen

    Recombinant fragment corresponding to Human Ku70 aa 6-214. Expressed in E. Coli.
    Sequence:

    SYYKT EGDEEAEEEQ EENLEASGDY KYSGRDSLIF LVDASKAMFE SQSEDELTPF DMSIQCIQSV YISKIISSDR DLLAVVFYGT EKDKNSVNFK NIYVLQELDN PGAKRILELD QFKGQQGQKR FQDMMGHGSD YSLSEVLWVC ANLFSDVQFK MSHKRIMLFT NEDNPHGNDS AKASRARTKA GDLRDTGIFL DLMHLKKPGG FDIS


    Database link: P12956

  • Positive control

    • Human recombinant Ku70 (aa6-214) protein; Human recombinant Ku70 (aa6-214)-hIgGFc transfected HEK293 cell lysate; PC2 , A549, A431, HepG and K562 cell lysates; A431 cells.

Properties

Applications

Our Abpromise guarantee covers the use of ab201963 in the following tested applications.

The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.

Application Abreviews Notes
Flow Cyt 1/200 - 1/400.

ab170190 - Mouse monoclonal IgG1, is suitable for use as an isotype control with this antibody.

 

WB 1/500 - 1/2000. Predicted molecular weight: 70 kDa.

Target

  • Function

    Single stranded DNA-dependent ATP-dependent helicase. Has a role in chromosome translocation. The DNA helicase II complex binds preferentially to fork-like ends of double-stranded DNA in a cell cycle-dependent manner. It works in the 3'-5' direction. Binding to DNA may be mediated by XRCC6. Involved in DNA non-homologous end joining (NHEJ) required for double-strand break repair and V(D)J recombination. The XRCC5/6 dimer acts as regulatory subunit of the DNA-dependent protein kinase complex DNA-PK by increasing the affinity of the catalytic subunit PRKDC to DNA by 100-fold. The XRCC5/6 dimer is probably involved in stabilizing broken DNA ends and bringing them together. The assembly of the DNA-PK complex to DNA ends is required for the NHEJ ligation step. Required for osteocalcin gene expression. Probably also acts as a 5'-deoxyribose-5-phosphate lyase (5'-dRP lyase), by catalyzing the beta-elimination of the 5' deoxyribose-5-phosphate at an abasic site near double-strand breaks. 5'-dRP lyase activity allows to 'clean' the termini of abasic sites, a class of nucleotide damage commonly associated with strand breaks, before such broken ends can be joined. The XRCC5/6 dimer together with APEX1 acts as a negative regulator of transcription.
  • Sequence similarities

    Belongs to the ku70 family.
    Contains 1 Ku domain.
    Contains 1 SAP domain.
  • Developmental stage

    Expression does not increase during promyelocyte differentiation.
  • Post-translational
    modifications

    Phosphorylation by PRKDC may enhance helicase activity. Phosphorylation of Ser-51 does not affect DNA repair.
  • Cellular localization

    Nucleus. Chromosome.
  • Information by UniProt
  • Database links

  • Alternative names

    • 5''-deoxyribose-5-phosphate lyase Ku70 antibody
    • 5''-dRP lyase Ku70 antibody
    • 70 kDa subunit of Ku antigen antibody
    • ATP dependent DNA helicase 2 subunit 1 antibody
    • ATP dependent DNA helicase II 70 kDa subunit antibody
    • ATP-dependent DNA helicase 2 subunit 1 antibody
    • ATP-dependent DNA helicase II 70 kDa subunit antibody
    • CTC box binding factor 75 kDa subunit antibody
    • CTC box-binding factor 75 kDa subunit antibody
    • CTC75 antibody
    • CTCBF antibody
    • DNA repair protein XRCC6 antibody
    • G22P1 antibody
    • Ku 70 antibody
    • Ku autoantigen p70 subunit antibody
    • Ku autoantigen, 70kDa antibody
    • Ku p70 antibody
    • Ku70 antibody
    • Ku70 DNA binding component of DNA-dependent proteinkinase complex (thyroid autoantigen 70 kDa antibody
    • Kup70 antibody
    • Lupus Ku autoantigen protein p70 antibody
    • ML8 antibody
    • Thyroid autoantigen 70kD (Ku antigen) antibody
    • Thyroid autoantigen antibody
    • Thyroid lupus autoantigen antibody
    • Thyroid lupus autoantigen p70 antibody
    • Thyroid-lupus autoantigen antibody
    • TLAA antibody
    • X ray repair complementing defective repair in Chinese hamster cells 6 antibody
    • X-ray repair complementing defective repair in Chinese hamster cells 6 antibody
    • X-ray repair cross-complementing protein 6 antibody
    • XRCC 6 antibody
    • Xrcc6 antibody
    • XRCC6_HUMAN antibody
    see all

Images

  • Flow cytometric analysis of A431 cells labeling Ku70 with ab201963 at 1/200 dilution (green). Negative control (red).

  • All lanes : Anti-Ku70 antibody [7A9E7] (ab201963) at 1/500 dilution

    Lane 1 : PC2 cell lysate
    Lane 2 : A549 cell lysate
    Lane 3 : A431 cell lysate
    Lane 4 : HepG2 cell lysate
    Lane 5 : K562 cell lysate

    Predicted band size: 70 kDa

  • All lanes : Anti-Ku70 antibody [7A9E7] (ab201963) at 1/500 dilution

    Lane 1 : HEK293 cell lysate
    Lane 2 : Human recombinant Ku70 (aa6-214)-hIgGFc transfected HEK293 cell lysate

    Predicted band size: 70 kDa

  • Anti-Ku70 antibody [7A9E7] (ab201963) at 1/500 dilution + Human recombinant Ku70 (aa6-214) protein

    Predicted band size: 70 kDa



    (Expected MW is 49.7 kDa)

References

ab201963 has not yet been referenced specifically in any publications.

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