Anti-Ku70 + Ku80 antibody - C-terminal (ab166822)
Key features and details
- Rabbit polyclonal to Ku70 + Ku80 - C-terminal
- Suitable for: WB, IHC-P, ICC/IF
- Reacts with: Human
- Isotype: IgG
Get better batch-to-batch reproducibility with a recombinant antibody
- Research with confidence – consistent and reproducible results with every batch
- Long-term and scalable supply – powered by recombinant technology for fast production
- Success from the first experiment – confirmed specificity through extensive validation
- Ethical standards compliant – production is animal-free
Overview
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Product name
Anti-Ku70 + Ku80 antibody - C-terminal
See all Ku70 + Ku80 primary antibodies -
Description
Rabbit polyclonal to Ku70 + Ku80 - C-terminal -
Host species
Rabbit -
Tested applications
Suitable for: WB, IHC-P, ICC/IFmore details -
Species reactivity
Reacts with: Human -
Immunogen
Synthetic peptide corresponding to a region within the C terminal of Human Ku70 + Ku80 (P12956, P13010).
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Positive control
- LOVO cell extracts; Human kidney tissue; A549 cells.
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General notes
The Life Science industry has been in the grips of a reproducibility crisis for a number of years. Abcam is leading the way in addressing this with our range of recombinant monoclonal antibodies and knockout edited cell lines for gold-standard validation. Please check that this product meets your needs before purchasing.
If you have any questions, special requirements or concerns, please send us an inquiry and/or contact our Support team ahead of purchase. Recommended alternatives for this product can be found below, along with publications, customer reviews and Q&As
Properties
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Form
Liquid -
Storage instructions
Shipped at 4°C. Store at -20°C. -
Storage buffer
pH: 7.40
Preservative: 0.02% Sodium azide
Constituents: 49% PBS, 50% Glycerol (glycerin, glycerine), 0.88% Sodium chloride
without Mg2+, Ca2+ -
Concentration information loading...
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Purity
Immunogen affinity purified -
Clonality
Polyclonal -
Isotype
IgG -
Research areas
Associated products
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Compatible Secondaries
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Isotype control
Applications
The Abpromise guarantee
Our Abpromise guarantee covers the use of ab166822 in the following tested applications.
The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.
Application | Abreviews | Notes |
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WB |
1/500 - 1/1000. Predicted molecular weight: 70, 82 kDa.
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IHC-P |
Use a concentration of 5 µg/ml. Perform heat mediated antigen retrieval with citrate buffer pH 6 before commencing with IHC staining protocol.
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ICC/IF |
1/100 - 1/500.
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Notes |
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WB
1/500 - 1/1000. Predicted molecular weight: 70, 82 kDa. |
IHC-P
Use a concentration of 5 µg/ml. Perform heat mediated antigen retrieval with citrate buffer pH 6 before commencing with IHC staining protocol. |
ICC/IF
1/100 - 1/500. |
Target
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Relevance
The Ku autoantigen is a heterodimer of 70kDa (p70) and 80kDa (p80) proteins. The p70 / p80 dimer is important for function of a 460kDa DNA-dependent protein kinase that phosphorylates certain transcription factors, including Sp1, Oct-1, p53, and SV40 large T antigen in vitro. The Ku protein plays a role in cell signaling, proliferation, DNA repair, replication, transcriptional activation, and apoptosis. -
Cellular localization
Nuclear -
Database links
- Entrez Gene: 2547 Human
- Entrez Gene: 7520 Human
- Omim: 152690 Human
- Omim: 194364 Human
- SwissProt: P12956 Human
- SwissProt: P13010 Human
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Alternative names
- CTC75 antibody
- CTC85 antibody
- CTCBF antibody
see all
Images
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All lanes : Anti-Ku70 + Ku80 antibody - C-terminal (ab166822) at 1/500 dilution
Lane 1 : LoVo cell lysate
Lane 2 : LoVo cell lysate with synthesized peptide
Predicted band size: 70, 82 kDa -
Immunohistochemical analysis of formalin fixed, paraffin embedded Human kidney tissue labeling Ku70 + Ku80 with ab166822 at 5 µg/ml.
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Immunofluorescence analysis of A549 cells labeling Ku70 + Ku80 with ab166822 at 1/100. The panel on the right is treated with the synthesized peptide.
Protocols
Datasheets and documents
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SDS download
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Datasheet download
References (0)
ab166822 has not yet been referenced specifically in any publications.