Key features and details
- Rabbit polyclonal to Ku70 + Ku80 - C-terminal
- Suitable for: WB, IHC-P, ICC/IF
- Reacts with: Human
- Isotype: IgG
Product nameAnti-Ku70 + Ku80 antibody - C-terminal
See all Ku70 + Ku80 primary antibodies
DescriptionRabbit polyclonal to Ku70 + Ku80 - C-terminal
Tested applicationsSuitable for: WB, IHC-P, ICC/IFmore details
Species reactivityReacts with: Human
Synthetic peptide corresponding to a region within the C terminal of Human Ku70 + Ku80 (P12956, P13010).
- LOVO cell extracts; Human kidney tissue; A549 cells.
Storage instructionsShipped at 4°C. Store at -20°C.
Storage bufferpH: 7.40
Preservative: 0.02% Sodium azide
Constituents: 49% PBS, 50% Glycerol, 0.88% Sodium chloride
without Mg2+, Ca2+
Concentration information loading...
PurityImmunogen affinity purified
Our Abpromise guarantee covers the use of ab166822 in the following tested applications.
The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.
|WB||1/500 - 1/1000. Predicted molecular weight: 70, 82 kDa.|
|IHC-P||Use a concentration of 5 µg/ml. Perform heat mediated antigen retrieval with citrate buffer pH 6 before commencing with IHC staining protocol.|
|ICC/IF||1/100 - 1/500.|
RelevanceThe Ku autoantigen is a heterodimer of 70kDa (p70) and 80kDa (p80) proteins. The p70 / p80 dimer is important for function of a 460kDa DNA-dependent protein kinase that phosphorylates certain transcription factors, including Sp1, Oct-1, p53, and SV40 large T antigen in vitro. The Ku protein plays a role in cell signaling, proliferation, DNA repair, replication, transcriptional activation, and apoptosis.
- CTC75 antibody
- CTC85 antibody
- CTCBF antibody
All lanes : Anti-Ku70 + Ku80 antibody - C-terminal (ab166822) at 1/500 dilution
Lane 1 : LoVo cell lysate
Lane 2 : LoVo cell lysate with synthesized peptide
Predicted band size: 70, 82 kDa
Immunohistochemical analysis of formalin fixed, paraffin embedded Human kidney tissue labeling Ku70 + Ku80 with ab166822 at 5 µg/ml.
Immunofluorescence analysis of A549 cells labeling Ku70 + Ku80 with ab166822 at 1/100. The panel on the right is treated with the synthesized peptide.
ab166822 has not yet been referenced specifically in any publications.