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Rabbit polyclonal to Ku70 + Ku80 - C-terminal
Synthetic peptide corresponding to a region within the C terminal of Human Ku70 + Ku80 (P12956, P13010).
LOVO cell extracts; Human kidney tissue; A549 cells.
Shipped at 4°C. Store at -20°C.
Preservative: 0.02% Sodium azide Constituents: 49% PBS, 50% Glycerol, 0.88% Sodium chloride Note: without Mg 2+, Ca 2+
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Immunogen affinity purified
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in the following tested applications.
The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.
1/500 - 1/1000. Predicted molecular weight: 70, 82 kDa.
Use a concentration of 5 µg/ml. Perform heat mediated antigen retrieval with citrate buffer pH 6 before commencing with IHC staining protocol.
1/100 - 1/500.
The Ku autoantigen is a heterodimer of 70kDa (p70) and 80kDa (p80) proteins. The p70 / p80 dimer is important for function of a 460kDa DNA-dependent protein kinase that phosphorylates certain transcription factors, including Sp1, Oct-1, p53, and SV40 large T antigen in vitro. The Ku protein plays a role in cell signaling, proliferation, DNA repair, replication, transcriptional activation, and apoptosis.
Western blot - Anti-Ku70 + Ku80 antibody - C-terminal (ab166822)
All lanes : Anti-Ku70 + Ku80 antibody - C-terminal (ab166822) at 1/500 dilution Lane 1 : LoVo cell lysate Lane 2 : LoVo cell lysate with synthesized peptide Predicted band size: 70, 82 kDa
Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-Ku70 + Ku80 antibody - C-terminal (ab166822)
Immunohistochemical analysis of formalin fixed, paraffin embedded Human kidney tissue labeling Ku70 + Ku80 with ab166822 at 5 µg/ml.
Immunocytochemistry/ Immunofluorescence - Anti-Ku70 + Ku80 antibody - C-terminal (ab166822)
Immunofluorescence analysis of A549 cells labeling Ku70 + Ku80 with ab166822 at 1/100. The panel on the right is treated with the synthesized peptide.
has not yet been referenced specifically in any publications.
Please note: All products are "FOR RESEARCH USE ONLY AND ARE NOT INTENDED FOR DIAGNOSTIC OR THERAPEUTIC USE"