Key features and details
- Rabbit polyclonal to Ku70 (phospho S5)
- Suitable for: WB, IHC-P
- Reacts with: Human
- Isotype: IgG
Product nameAnti-Ku70 (phospho S5) antibody
See all Ku70 primary antibodies
DescriptionRabbit polyclonal to Ku70 (phospho S5)
SpecificityDetects endogenous levels of Ku70 only when phosphorylated at serine 5 (mouse homologue is Serine 6).
Tested applicationsSuitable for: WB, IHC-Pmore details
Species reactivityReacts with: Human
Predicted to work with: Mouse
Synthetic phosphopeptide derived from human Ku70 around the phosphorylation site of serine 5 (WESPYY)
- Extracts from HeLa cells (WB) Human lung carcinoma tissue (IHC)
Reproducibility is key to advancing scientific discovery and accelerating scientists’ next breakthrough.
Abcam is leading the way with our range of recombinant antibodies, knockout-validated antibodies and knockout cell lines, all of which support improved reproducibility.
We are also planning to innovate the way in which we present recommended applications and species on our product datasheets, so that only applications & species that have been tested in our own labs, our suppliers or by selected trusted collaborators are covered by our Abpromise™ guarantee.
In preparation for this, we have started to update the applications & species that this product is Abpromise guaranteed for.
We are also updating the applications & species that this product has been “predicted to work with,” however this information is not covered by our Abpromise guarantee.
Applications & species from publications and Abreviews that have not been tested in our own labs or in those of our suppliers are not covered by the Abpromise guarantee.
Please check that this product meets your needs before purchasing. If you have any questions, special requirements or concerns, please send us an inquiry and/or contact our Support team ahead of purchase. Recommended alternatives for this product can be found below, as well as customer reviews and Q&As.
Storage instructionsShipped at 4°C. Upon delivery aliquot and store at -20°C. Avoid freeze / thaw cycles.
Storage bufferpH: 7.40
Preservative: 0.02% Sodium azide
Constituents: PBS, 50% Glycerol (glycerin, glycerine), 0.87% Sodium chloride
Without Mg2+ and Ca2+
Concentration information loading...
PurityImmunogen affinity purified
Purification notesAffinity purified from rabbit antiserum by affinity chromatography using epitope specific phosphopeptide. The antibody against non phosphopeptide was removed by chromatography using non phosphopeptide corresponding to the phosphorylation site.
Our Abpromise guarantee covers the use of ab61783 in the following tested applications.
The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.
|WB||1/500 - 1/1000. Predicted molecular weight: 70 kDa.|
|IHC-P||1/50 - 1/100.|
FunctionSingle stranded DNA-dependent ATP-dependent helicase. Has a role in chromosome translocation. The DNA helicase II complex binds preferentially to fork-like ends of double-stranded DNA in a cell cycle-dependent manner. It works in the 3'-5' direction. Binding to DNA may be mediated by XRCC6. Involved in DNA non-homologous end joining (NHEJ) required for double-strand break repair and V(D)J recombination. The XRCC5/6 dimer acts as regulatory subunit of the DNA-dependent protein kinase complex DNA-PK by increasing the affinity of the catalytic subunit PRKDC to DNA by 100-fold. The XRCC5/6 dimer is probably involved in stabilizing broken DNA ends and bringing them together. The assembly of the DNA-PK complex to DNA ends is required for the NHEJ ligation step. Required for osteocalcin gene expression. Probably also acts as a 5'-deoxyribose-5-phosphate lyase (5'-dRP lyase), by catalyzing the beta-elimination of the 5' deoxyribose-5-phosphate at an abasic site near double-strand breaks. 5'-dRP lyase activity allows to 'clean' the termini of abasic sites, a class of nucleotide damage commonly associated with strand breaks, before such broken ends can be joined. The XRCC5/6 dimer together with APEX1 acts as a negative regulator of transcription.
Sequence similaritiesBelongs to the ku70 family.
Contains 1 Ku domain.
Contains 1 SAP domain.
Developmental stageExpression does not increase during promyelocyte differentiation.
modificationsPhosphorylation by PRKDC may enhance helicase activity. Phosphorylation of Ser-51 does not affect DNA repair.
Cellular localizationNucleus. Chromosome.
- Information by UniProt
- 5''-deoxyribose-5-phosphate lyase Ku70 antibody
- 5''-dRP lyase Ku70 antibody
- 70 kDa subunit of Ku antigen antibody
Immunohistochemistry analysis of paraffin embedded human lung carcinoma tissue using ab61783 at 1/50 dilution in the presence and abscence of immunizing peptide antibody.
All lanes : Anti-Ku70 (phospho S5) antibody (ab61783) at 1/500 dilution
Lane 1 : extracts from HeLa cells with no immunizing peptide
Lane 2 : extracts from HeLa cells with immunizing peptide
Predicted band size: 70 kDa
Observed band size: 70 kDa
ab61783 has been referenced in 4 publications.
- Timm S et al. Clustered DNA damage concentrated in particle trajectories causes persistent large-scale rearrangements in chromatin architecture. Radiother Oncol N/A:N/A (2018). PubMed: 30049456
- Natale F et al. Identification of the elementary structural units of the DNA damage response. Nat Commun 8:15760 (2017). PubMed: 28604675
- Schuler N et al. DNA-damage foci to detect and characterize DNA repair alterations in children treated for pediatric malignancies. PLoS One 9:e91319 (2014). IHC ; Human . PubMed: 24637877
- Schuler N & Rübe CE Accumulation of DNA damage-induced chromatin alterations in tissue-specific stem cells: the driving force of aging? PLoS One 8:e63932 (2013). Flow Cyt ; Mouse . PubMed: 23691119