Key features and details
- Rabbit polyclonal to Ku80 - C-terminal
- Suitable for: IHC-P
- Reacts with: Human
- Isotype: IgG
Product nameAnti-Ku80 antibody - C-terminal
See all Ku80 primary antibodies
DescriptionRabbit polyclonal to Ku80 - C-terminal
Tested applicationsSuitable for: IHC-Pmore details
Species reactivityReacts with: Human
Synthetic peptide corresponding to Human Ku80 (C terminal).
Database link: P13010
- IHC-P: Human placenta tissue.
This product is FOR RESEARCH USE ONLY. For commercial use, please contact firstname.lastname@example.org.
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In preparation for this, we have started to update the applications & species that this product is Abpromise guaranteed for.
We are also updating the applications & species that this product has been “predicted to work with,” however this information is not covered by our Abpromise guarantee.
Applications & species from publications and Abreviews that have not been tested in our own labs or in those of our suppliers are not covered by the Abpromise guarantee.
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Storage instructionsShipped at 4°C. Store at +4°C short term (1-2 weeks). Upon delivery aliquot. Store at -20°C long term. Avoid freeze / thaw cycle.
Storage bufferpH: 7.60
Preservative: 0.1% Sodium azide
Constituents: PBS, 1% BSA
Concentration information loading...
PurityImmunogen affinity purified
Our Abpromise guarantee covers the use of ab227792 in the following tested applications.
The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.
Perform heat mediated antigen retrieval with 1 mM EDTA buffer pH 8.0 before commencing with IHC staining protocol.
Primary antibody incubation for 10 minutes at room temperature.
FunctionSingle stranded DNA-dependent ATP-dependent helicase. Has a role in chromosome translocation. The DNA helicase II complex binds preferentially to fork-like ends of double-stranded DNA in a cell cycle-dependent manner. It works in the 3'-5' direction. Binding to DNA may be mediated by XRCC6. Involved in DNA non-homologous end joining (NHEJ) required for double-strand break repair and V(D)J recombination. The XRCC5/6 dimer acts as regulatory subunit of the DNA-dependent protein kinase complex DNA-PK by increasing the affinity of the catalytic subunit PRKDC to DNA by 100-fold. The XRCC5/6 dimer is probably involved in stabilizing broken DNA ends and bringing them together. The assembly of the DNA-PK complex to DNA ends is required for the NHEJ ligation step. In association with NAA15, the XRCC5/6 dimer binds to the osteocalcin promoter and activates osteocalcin expression. The XRCC5/6 dimer probably also acts as a 5'-deoxyribose-5-phosphate lyase (5'-dRP lyase), by catalyzing the beta-elimination of the 5' deoxyribose-5-phosphate at an abasic site near double-strand breaks. XRCC5 probably acts as the catalytic subunit of 5'-dRP activity, and allows to 'clean' the termini of abasic sites, a class of nucleotide damage commonly associated with strand breaks, before such broken ends can be joined. The XRCC5/6 dimer together with APEX1 acts as a negative regulator of transcription.
Sequence similaritiesBelongs to the ku80 family.
Contains 1 Ku domain.
Developmental stageExpression increases during promyelocyte differentiation.
DomainThe EEXXXDDL motif is required for the interaction with catalytic subunit PRKDC and its recruitment to sites of DNA damage.
modificationsPhosphorylated on serine residues. Phosphorylation by PRKDC may enhance helicase activity.
Cellular localizationNucleus. Chromosome.
- Information by UniProt
- 86 kDa subunit of Ku antigen antibody
- ATP dependent DNA helicase 2 subunit 2 antibody
- ATP dependent DNA helicase II 80 kDa subunit antibody
To our knowledge, customised protocols are not required for this product. Please try the standard protocols listed below and let us know how you get on.
ab227792 has not yet been referenced specifically in any publications.