Anti-Ku80 antibody [EPR3467] (ab79391)


  • Product name
    Anti-Ku80 antibody [EPR3467]
    See all Ku80 primary antibodies
  • Description
    Rabbit monoclonal [EPR3467] to Ku80
  • Host species
  • Specificity
    The immunogen for ab79391 has 78.6% homology with mouse and rat ku80, therefore we do not guarantee reactivity in these species.
  • Tested applications
    Suitable for: ICC/IF, WB, IHC-P, Flow Cytmore details
    Unsuitable for: IP
  • Species reactivity
    Reacts with: Human
  • Immunogen

    Synthetic peptide within Human Ku80 aa 50-150. The exact sequence is proprietary.

  • Positive control
    • A549 (treated with FBS), HeLa, U937 and HepG2 cell lysates; human breast carcinoma tissue.
  • General notes

    Mouse, Rat: We have preliminary internal testing data to indicate this antibody may not react with these species. Please contact us for more information.


    Our RabMAb® technology is a patented hybridoma-based technology for making rabbit monoclonal antibodies. For details on our patents, please refer to RabMab® patents

    This product is a recombinant rabbit monoclonal antibody.


  • Form
  • Storage instructions
    Shipped at 4°C. Store at +4°C short term (1-2 weeks). Upon delivery aliquot. Store at -20°C. Stable for 12 months at -20°C.
  • Storage buffer
    pH: 7.20
    Preservative: 0.05% Sodium azide
    Constituents: 0.1% BSA, 40% Glycerol, 9.85% Tris glycine, 50% Tissue culture supernatant
  • Purity
    Tissue culture supernatant
  • Clonality
  • Clone number
  • Isotype
  • Research areas


Our Abpromise guarantee covers the use of ab79391 in the following tested applications.

The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.

Application Abreviews Notes
ICC/IF 1/100 - 1/250.
WB 1/500 - 1/2000. Detects a band of approximately 83 kDa (predicted molecular weight: 83 kDa).
IHC-P 1/250 - 1/500. Perform heat mediated antigen retrieval before commencing with IHC staining protocol.
Flow Cyt 1/150.

ab172730 - Rabbit monoclonal IgG, is suitable for use as an isotype control with this antibody.

  • Application notes
    Is unsuitable for IP.
  • Target

    • Function
      Single stranded DNA-dependent ATP-dependent helicase. Has a role in chromosome translocation. The DNA helicase II complex binds preferentially to fork-like ends of double-stranded DNA in a cell cycle-dependent manner. It works in the 3'-5' direction. Binding to DNA may be mediated by XRCC6. Involved in DNA non-homologous end joining (NHEJ) required for double-strand break repair and V(D)J recombination. The XRCC5/6 dimer acts as regulatory subunit of the DNA-dependent protein kinase complex DNA-PK by increasing the affinity of the catalytic subunit PRKDC to DNA by 100-fold. The XRCC5/6 dimer is probably involved in stabilizing broken DNA ends and bringing them together. The assembly of the DNA-PK complex to DNA ends is required for the NHEJ ligation step. In association with NAA15, the XRCC5/6 dimer binds to the osteocalcin promoter and activates osteocalcin expression. The XRCC5/6 dimer probably also acts as a 5'-deoxyribose-5-phosphate lyase (5'-dRP lyase), by catalyzing the beta-elimination of the 5' deoxyribose-5-phosphate at an abasic site near double-strand breaks. XRCC5 probably acts as the catalytic subunit of 5'-dRP activity, and allows to 'clean' the termini of abasic sites, a class of nucleotide damage commonly associated with strand breaks, before such broken ends can be joined. The XRCC5/6 dimer together with APEX1 acts as a negative regulator of transcription.
    • Sequence similarities
      Belongs to the ku80 family.
      Contains 1 Ku domain.
    • Developmental stage
      Expression increases during promyelocyte differentiation.
    • Domain
      The EEXXXDDL motif is required for the interaction with catalytic subunit PRKDC and its recruitment to sites of DNA damage.
    • Post-translational
      Phosphorylated on serine residues. Phosphorylation by PRKDC may enhance helicase activity.
    • Cellular localization
      Nucleus. Chromosome.
    • Information by UniProt
    • Database links
    • Alternative names
      • 86 kDa subunit of Ku antigen antibody
      • ATP dependent DNA helicase 2 subunit 2 antibody
      • ATP dependent DNA helicase II 80 kDa subunit antibody
      • ATP dependent DNA helicase II 86 Kd subunit antibody
      • ATP dependent DNA helicase II antibody
      • ATP-dependent DNA helicase 2 subunit 2 antibody
      • ATP-dependent DNA helicase II 80 kDa subunit antibody
      • CTC box binding factor 85 kDa antibody
      • CTC box-binding factor 85 kDa subunit antibody
      • CTC85 antibody
      • CTCBF antibody
      • DNA repair protein XRCC5 antibody
      • KARP 1 antibody
      • KARP1 antibody
      • Ku 80 antibody
      • Ku autoantigen 80kDa antibody
      • Ku80 antibody
      • Ku86 antibody
      • Ku86 autoantigen related protein 1 antibody
      • KUB 2 antibody
      • KUB2 antibody
      • Lupus Ku autoantigen protein p86 antibody
      • NFIV antibody
      • Nuclear factor IV antibody
      • Thyroid lupus autoantigen antibody
      • Thyroid-lupus autoantigen antibody
      • TLAA antibody
      • X ray repair complementing defective repair in Chinese hamster cells 5 (double strand break rejoining) antibody
      • X-ray repair complementing defective repair in Chinese hamster cells 5 (double-strand-break rejoining) antibody
      • X-ray repair cross-complementing protein 5 antibody
      • Xray repair complementing defective repair in Chinese hamster cells 5 antibody
      • XRCC 5 antibody
      • XRCC5 antibody
      • XRCC5_HUMAN antibody
      see all


    • All lanes : Anti-Ku80 antibody [EPR3467] (ab79391) at 1/2000 dilution

      Lane 1 : A549 cell lysate, treated with FBS

      Lane 2 : HeLa cell lysate
      Lane 3 : U937 cell lysate
      Lane 4 : HepG2 cell lysate

      Lysates/proteins at 10 µg per lane.

      All lanes : goat anti-rabbit HRP at 1/2000 dilution

      Predicted band size: 83 kDa
      Observed band size: 83 kDa

    • ab79391 (1/200) staining Ku80 in Hela cells (green). Cells were fixed in methanol and counterstained with DAPI in order to highlight the nucleus (red). For further experimental details please refer to Abreview.

      See Abreview

    • Overlay histogram showing HeLa cells stained with ab79391 (red line). The cells were fixed with 80% methanol (5 min) and then permeabilized with 0.1% PBS-Tween for 20 min. The cells were then incubated in 1x PBS / 10% normal goat serum / 0.3M glycine to block non-specific protein-protein interactions followed by the antibody (ab79391, 1/100 dilution) for 30 min at 22°C. The secondary antibody used was Alexa Fluor® 488 goat anti-rabbit IgG (H+L) (ab150077) at 1/2000 dilution for 30 min at 22°C. Isotype control antibody (black line) was rabbit IgG (monoclonal) (1μg/1x106 cells) used under the same conditions. Unlabelled sample (blue line) was also used as a control. Acquisition of >5,000 events were collected using a 20mW Argon ion laser (488nm) and 525/30 bandpass filter.
    • ab79391 at 1/250 dilution staining Ku80 in human breast carcinoma by Immunohistochemistry, Paraffin-embedded tissue.


    ab79391 has not yet been referenced specifically in any publications.

    Customer reviews and Q&As

    Abcam guarantees this product to work in the species/application used in this Abreview.
    Human Cell (HeLa)

    Dr. Kirk Mcmanus

    Verified customer

    Submitted Feb 10 2012

    Thank you for contacting us.

    I can confirm that the abs have IgG1 isotype.

    I hope this information is helpful to you. Please do not hesitate to contact us if you need any more advice or information.

    Thank you for contacting us and your interest in our products.

    As far as we know, rabbits do not have specific IgG subclasses like mice or humans. This rabbit monoclonal is therefore simply of the IgG class.

    I hope this informatio...

    Read More
    Abcam has not validated the combination of species/application used in this Abreview.
    Immunocytochemistry/ Immunofluorescence
    Human Cell (HeLa)

    Dr. Kirk Mcmanus

    Verified customer

    Submitted Nov 16 2009


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