Product nameAnti-Kv1.1 potassium channel antibody
See all Kv1.1 potassium channel primary antibodies
DescriptionRabbit polyclonal to Kv1.1 potassium channel
Tested applicationsSuitable for: IHC-P, IHC-Fr, WB, IHC-FoFr, ICC/IFmore details
Species reactivityReacts with: Human
Predicted to work with: Mouse, Rat
Synthetic peptide derived from the C terminal domain of human Kv1.1 potassium channel.
Storage instructionsShipped at 4°C. Upon delivery aliquot and store at -20°C. Avoid freeze / thaw cycles.
Storage bufferConstituent: Whole serum
Our Abpromise guarantee covers the use of ab65790 in the following tested applications.
The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.
|IHC-P||Use at an assay dependent concentration.|
|IHC-Fr||Use at an assay dependent concentration.|
|WB||1/1000 - 1/5000. Detects a band of approximately 57 kDa (predicted molecular weight: 57 kDa).|
|IHC-FoFr||Use at an assay dependent concentration. PubMed: 21752989|
|ICC/IF||Use at an assay dependent concentration. PubMed: 19779067|
FunctionMediates the voltage-dependent potassium ion permeability of excitable membranes. Assuming opened or closed conformations in response to the voltage difference across the membrane, the protein forms a potassium-selective channel through which potassium ions may pass in accordance with their electrochemical gradient.
Involvement in diseaseDefects in KCNA1 are the cause of episodic ataxia type 1 (EA1) [MIM:160120]; also known as paroxysmal or episodic ataxia with myokymia (EAM) or paroxysmal ataxia with neuromyotonia. EA1 is an autosomal dominant disorder characterized by brief episodes of ataxia and dysarthria. Neurological examination during and between the attacks demonstrates spontaneous, repetitive discharges in the distal musculature (myokymia) that arise from peripheral nerve. Nystagmus is absent.
Defects in KCNA1 are the cause of myokymia isolated type 1 (MK1) [MIM:160120]. Myokymia is a condition characterized by spontaneous involuntary contraction of muscle fiber groups that can be observed as vermiform movement of the overlying skin. Electromyography typically shows continuous motor unit activity with spontaneous oligo- and multiplet-discharges of high intraburst frequency (myokymic discharges). Isolated spontaneous muscle twitches occur in many persons and have no grave significance.
Sequence similaritiesBelongs to the potassium channel family. A (Shaker) (TC 1.A.1.2) subfamily. Kv1.1/KCNA1 sub-subfamily.
DomainThe N-terminus may be important in determining the rate of inactivation of the channel while the tail may play a role in modulation of channel activity and/or targeting of the channel to specific subcellular compartments.
The segment S4 is probably the voltage-sensor and is characterized by a series of positively charged amino acids at every third position.
modificationsPalmitoylated on Cys-243; which may be required for membrane targeting.
- Information by UniProt
- AEMK antibody
- EA1 antibody
- Episodic ataxia with myokymia antibody
All lanes : Anti-Kv1.1 potassium channel antibody (ab65790) at 1/500 dilution
Lane 1 : Human brain tissue lysate - total protein (ab29466)
Lane 2 : Human testis tissue lysate - total protein (ab30257)
Lysates/proteins at 10 µg per lane.
All lanes : Goat polyclonal to Rabbit IgG - H&L - Pre-Adsorbed (HRP) at 1/3000 dilution
Developed using the ECL technique.
Performed under reducing conditions.
Predicted band size: 57 kDa
Observed band size: 57 kDa
Additional bands at: 73 kDa. We are unsure as to the identity of these extra bands.
Exposure time: 3 minutes
This product has been referenced in:
- Viader A et al. Schwann cell mitochondrial metabolism supports long-term axonal survival and peripheral nerve function. J Neurosci 31:10128-40 (2011). IHC-FoFr ; Mouse . Read more (PubMed: 21752989) »
- Heeroma JH et al. Episodic ataxia type 1 mutations differentially affect neuronal excitability and transmitter release. Dis Model Mech 2:612-9 (2009). ICC/IF ; Rat . Read more (PubMed: 19779067) »