Recombinant
RabMAb

Recombinant Anti-Kv1.1 potassium channel antibody [EPR12718] (ab177481)

Overview

  • Product name

    Anti-Kv1.1 potassium channel antibody [EPR12718]
    See all Kv1.1 potassium channel primary antibodies
  • Description

    Rabbit monoclonal [EPR12718] to Kv1.1 potassium channel
  • Host species

    Rabbit
  • Tested applications

    Suitable for: IP, WBmore details
    Unsuitable for: ICC/IF or IHC-P
  • Species reactivity

    Reacts with: Human
  • Immunogen

    Synthetic peptide (the amino acid sequence is considered to be commercially sensitive) within Human Kv1.1 potassium channel aa 400 to the C-terminus (Cysteine residue). The exact sequence is proprietary.
    Database link: Q09470

  • Positive control

    • Human fetal brain and Human fetal heart lysates.
  • General notes

    Mouse, Rat: We have preliminary internal testing data to indicate this antibody may not react with these species. Please contact us for more information.

    Our RabMAb® technology is a patented hybridoma-based technology for making rabbit monoclonal antibodies. For details on our patents, please refer to RabMab® patents.

    This product is a recombinant rabbit monoclonal antibody.

Properties

Applications

Our Abpromise guarantee covers the use of ab177481 in the following tested applications.

The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.

Application Abreviews Notes
IP 1/10 - 1/100.
WB 1/10000 - 1/50000. Detects a band of approximately 60 kDa (predicted molecular weight: 56 kDa).
  • Application notes
    Is unsuitable for ICC/IF or IHC-P.
  • Target

    • Function

      Mediates the voltage-dependent potassium ion permeability of excitable membranes. Assuming opened or closed conformations in response to the voltage difference across the membrane, the protein forms a potassium-selective channel through which potassium ions may pass in accordance with their electrochemical gradient.
    • Involvement in disease

      Defects in KCNA1 are the cause of episodic ataxia type 1 (EA1) [MIM:160120]; also known as paroxysmal or episodic ataxia with myokymia (EAM) or paroxysmal ataxia with neuromyotonia. EA1 is an autosomal dominant disorder characterized by brief episodes of ataxia and dysarthria. Neurological examination during and between the attacks demonstrates spontaneous, repetitive discharges in the distal musculature (myokymia) that arise from peripheral nerve. Nystagmus is absent.
      Defects in KCNA1 are the cause of myokymia isolated type 1 (MK1) [MIM:160120]. Myokymia is a condition characterized by spontaneous involuntary contraction of muscle fiber groups that can be observed as vermiform movement of the overlying skin. Electromyography typically shows continuous motor unit activity with spontaneous oligo- and multiplet-discharges of high intraburst frequency (myokymic discharges). Isolated spontaneous muscle twitches occur in many persons and have no grave significance.
    • Sequence similarities

      Belongs to the potassium channel family. A (Shaker) (TC 1.A.1.2) subfamily. Kv1.1/KCNA1 sub-subfamily.
    • Domain

      The N-terminus may be important in determining the rate of inactivation of the channel while the tail may play a role in modulation of channel activity and/or targeting of the channel to specific subcellular compartments.
      The segment S4 is probably the voltage-sensor and is characterized by a series of positively charged amino acids at every third position.
    • Post-translational
      modifications

      Palmitoylated on Cys-243; which may be required for membrane targeting.
    • Cellular localization

      Membrane.
    • Information by UniProt
    • Database links

    • Alternative names

      • AEMK antibody
      • EA1 antibody
      • Episodic ataxia with myokymia antibody
      • HBK1 antibody
      • HUK1 antibody
      • Kca1 1 antibody
      • Kcna1 antibody
      • KCNA1_HUMAN antibody
      • Kcpvd antibody
      • KV1.1 antibody
      • MBK1 antibody
      • mceph antibody
      • MGC124402 antibody
      • MGC126782 antibody
      • MGC138385 antibody
      • MK1 antibody
      • MK1, mouse, homolog of KV1.1 antibody
      • Potassium channel protein 1 antibody
      • Potassium voltage gated channel shaker related subfamily member 1 antibody
      • Potassium voltage gated channel subfamily A member 1 antibody
      • Potassium voltage gated channel, shaker related subfamily, member 1 (episodic ataxia with myokymia) antibody
      • Potassium voltage-gated channel subfamily A member 1 antibody
      • RBK1 antibody
      • RCK1 antibody
      • Shak antibody
      • Shaker related subfamily member 1 antibody
      • Voltage gated potassium channel subunit Kv1.1 antibody
      • Voltage-gated K(+) channel HuKI antibody
      • Voltage-gated potassium channel HBK1 antibody
      • Voltage-gated potassium channel subunit Kv1.1 antibody
      see all

    Images

    • All lanes : Anti-Kv1.1 potassium channel antibody [EPR12718] (ab177481) at 1/10000 dilution

      Lane 1 : Human fetal brain lysate
      Lane 2 : Human fetal heart lysate

      Lysates/proteins at 10 µg per lane.

      Secondary
      All lanes : HRP labelled goat anti-rabbit at 1/2000 dilution

      Predicted band size: 56 kDa

    • Immunoprecipitation of Kv1.1 potassium channel from Human fetal heart lysate using ab177481 at 1/10 or 1X PBS (negative control) (lane 2), and HRP-conjugated anti-rabbit IgG preferentially detecting the non-reduced form of rabbit IgG.

    References

    ab177481 has not yet been referenced specifically in any publications.

    Customer reviews and Q&As

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