Key features and details
- Rabbit polyclonal to Kv1.2 - Carboxyterminal end
- Suitable for: WB, ELISA, ICC/IF
- Reacts with: Recombinant fragment
- Isotype: IgG
Product nameAnti-Kv1.2 antibody - Carboxyterminal end
See all Kv1.2 primary antibodies
DescriptionRabbit polyclonal to Kv1.2 - Carboxyterminal end
Tested applicationsSuitable for: WB, ELISA, ICC/IFmore details
Species reactivityReacts with: Recombinant fragment
Predicted to work with: Mouse, Rat, Human
A synthetic peptide corresponding to C-terminal residues of mouse KCNA2
Storage instructionsShipped at 4°C. Upon delivery aliquot and store at -20°C. Avoid freeze / thaw cycles.
Storage bufferPreservative: 0.01% Sodium azide
Constituents: 50% Glycerol, PBS
Concentration information loading...
PurityImmunogen affinity purified
Our Abpromise guarantee covers the use of ab55987 in the following tested applications.
The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.
|WB||Use a concentration of 1 µg/ml. Predicted molecular weight: 57 kDa. This antibody has been tested in Western blot against the recombinant peptide used as an immunogen. We have no data on detection of endogenous protein.|
|ELISA||Use at an assay dependent dilution.|
|ICC/IF||Use a concentration of 1 - 5 µg/ml.|
RelevanceKv1.2 mediates the voltage-dependent potassium ion permeability of excitable membranes. Assuming opened or closed conformations in response to the voltage difference across the membrane, the protein forms a potassium-selective channel through which K(+) ions may pass in accordance with their electrochemical gradient. Kv1.2 binds PDZ domains of DLG1, DLG2 and DLG4. The N-terminus may be important in determining the rate of inactivation of the channel while the tail may play a role in modulation of channel activity and/or targeting of the channel to specific subcellular compartments.
Cellular localizationMembrane; Multi-pass membrane protein
- Akr6a4 antibody
- BK2 antibody
- HBK5 antibody
ICC/IF image of ab55987 stained SHSY5Y cells. The cells were 4% formaldehyde fixed (5 min) and then incubated in 1%BSA / 10% normal goat serum / 0.3M glycine in 0.1% PBS-Tween for 1h to permeabilise the cells and block non-specific protein-protein interactions. The cells were then incubated with the antibody (ab55987, 1µg/ml) overnight at +4°C. The secondary antibody (green) was Alexa Fluor® 488 goat anti-rabbit IgG (H+L) used at a 1/1000 dilution for 1h. Alexa Fluor® 594 WGA was used to label plasma membranes (red) at a 1/200 dilution for 1h. DAPI was used to stain the cell nuclei (blue) at a concentration of 1.43µM.
To our knowledge, customised protocols are not required for this product. Please try the standard protocols listed below and let us know how you get on.
ab55987 has been referenced in 1 publication.
- Zhang C et al. Effects of selenium on myocardial apoptosis by modifying the activity of mitochondrial STAT3 and regulating potassium channel expression. Exp Ther Med 14:2201-2205 (2017). PubMed: 28962142