Product nameAnti-Kv1.6/RCK2 antibody
DescriptionRabbit polyclonal to Kv1.6/RCK2
In human HEK293 cells ab21089 recognised a band of ~58 kDa, the predicted MW of Kv1.6/RCK2 (data not shown). This band was not blocked by the immunising peptide. Additional bands of 100 kDa, 25 kDa and 20 kDa were also recognised.
Tested applicationsSuitable for: ICC/IF, WB, IHC-FoFr, IHC-Pmore details
Species reactivityReacts with: Mouse, Rat
Predicted to work with: Human
Synthetic peptide corresponding to Human Kv1.6/RCK2 aa 500 to the C-terminus (C terminal) conjugated to keyhole limpet haemocyanin.
(Peptide available as
- This antibody gave a positive signal in Hek293 cells.
Storage instructionsShipped at 4°C. Store at +4°C short term (1-2 weeks). Upon delivery aliquot. Store at -20°C or -80°C. Avoid freeze / thaw cycle.
Storage bufferPreservative: 0.02% Sodium Azide
Constituents: 1% BSA, PBS, pH 7.4
Concentration information loading...
PurityImmunogen affinity purified
Our Abpromise guarantee covers the use of ab21089 in the following tested applications.
The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.
|ICC/IF||Use a concentration of 5 µg/ml.|
|WB||Use a concentration of 1 µg/ml. Detects a band of approximately 58 kDa (predicted molecular weight: 58 kDa).|
|IHC-P||1/40. Perform heat mediated antigen retrieval via the microwave method before commencing with IHC staining protocol.|
FunctionMediates the voltage-dependent potassium ion permeability of excitable membranes. Assuming opened or closed conformations in response to the voltage difference across the membrane, the protein forms a potassium-selective channel through which potassium ions may pass in accordance with their electrochemical gradient.
Sequence similaritiesBelongs to the potassium channel family. A (Shaker) (TC 1.A.1.2) subfamily. Kv1.6/KCNA6 sub-subfamily.
DomainThe N-terminus may be important in determining the rate of inactivation of the channel while the tail may play a role in modulation of channel activity and/or targeting of the channel to specific subcellular compartments.
The segment S4 is probably the voltage-sensor and is characterized by a series of positively charged amino acids at every third position.
- Information by UniProt
- HBK 2 antibody
- HBK2 antibody
- Human brain potassium channel 2 antibody
Image courtesy of Human Protein Atlas
ab21089 staining Kv6.1 in human pancreas tissue. Paraffin embedded human pancreas tissue was incubated with ab21089 (1/40 dilution) for 30 mins at room temperature. Antigen retrieval was performed by heat induction in citrate buffer pH 6.
ab21089 was tested in a tissue microarray (TMA) containing a wide range of normal and cancer tissues as well as a cell microarray consisting of a range of commonly used, well characterised human cell lines. Further results for this antibody can be found at www.proteinatlas.org
All lanes : Anti-Kv1.6/RCK2 antibody (ab21089) at 1 µg/ml
Lane 1 : Mouse Brain Lysate
Lane 2 : Mouse Brain Lysate Kv1.6/RCK2 with Human Kv1.6/RCK2 peptide (ab23431) at 1 µg/ml
Lysates/proteins at 20 µg per lane.
Lane 1 : Alexa Fluor Goat polyclonal to Rabbit IgG at 1/10000 dilution
Lane 2 : Alexa Fluor Goat polyclonal to Rabbit IgG
Predicted band size: 58 kDa
ab21089 recognises a band corresponding to the predicted MW of Kv1.6/RCK2 in mouse brain lysate (lane 1). Antibody binding to this band may be blocked using the immunising peptide, ab23431 (lane 2). The antibody also recognises additional proteins. These may be degradation/cleavage products of Kv1.6/RCK2, or they may represent non-specific binding by the antibody.
ICC/IF image of ab21089 stained human HEK 293 cells. The cells were PFA fixed (10 min), permabilised in TBS-T (20 min) and incubated with the antibody (ab21089, 5µg/ml) for 1h at room temperature. 1%BSA / 10% normal goat serum / 0.3M glycine was used to quench autofluorescence and block non-specific protein-protein interactions. The secondary antibody (green) was Alexa Fluor® 488 goat anti-rabbit IgG (H+L) used at a 1/1000 dilution for 1h. Alexa Fluor® 594 WGA was used to label plasma membranes (red). DAPI was used to stain the cell nuclei (blue).
The IHC-FoFr image stainig Kv1.6/RCK2(ab21089) on Rat DRG sections. The sections used came from animals perfused fixed with Paraformaldehyde 4%, in phosphate buffer 0.2M. Following postfixation in the same fixative overnight, the tissues were cryoprotected in sucrose 30% overnight. Tissues were then cut using a cryostat.
ab21089 has not yet been referenced specifically in any publications.