Overview

  • Product name

    Anti-Kv2.1/KCNB1 antibody [K89/34]
    See all Kv2.1/KCNB1 primary antibodies
  • Description

    Mouse monoclonal [K89/34] to Kv2.1/KCNB1
  • Host species

    Mouse
  • Tested applications

    Suitable for: WB, IHC-Pmore details
  • Species reactivity

    Reacts with: Mouse, Rat, Human
  • Immunogen

    Synthetic peptide corresponding to Rat Kv2.1/KCNB1 (C terminal).

  • Positive control

    • This antibody gave a positive result in IHC in the following FFPE tissue: Human normal Hippocampus
  • General notes

    This antibody clone is manufactured by Abcam. If you require a custom buffer formulation or conjugation for your experiments, please contact orders@abcam.com.

     

Properties

  • Form

    Liquid
  • Storage instructions

    Shipped at 4°C. Store at +4°C short term (1-2 weeks). Store at -20°C long term.
  • Storage buffer

    pH: 7.40
    Preservative: 0.02% Sodium azide
    Constituents: PBS, 6.97% L-Arginine
  • Concentration information loading...
  • Purity

    Protein G purified
  • Clonality

    Monoclonal
  • Clone number

    K89/34
  • Isotype

    IgG1
  • Light chain type

    kappa
  • Research areas

Applications

Our Abpromise guarantee covers the use of ab192761 in the following tested applications.

The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.

Application Abreviews Notes
WB Use a concentration of 1 - 5 µg/ml. Detects a band of approximately 98 kDa (predicted molecular weight: 95 kDa).
IHC-P Use a concentration of 1 µg/ml.

Target

  • Function

    Mediates the voltage-dependent potassium ion permeability of excitable membranes. Channels open or close in response to the voltage difference across the membrane, letting potassium ions pass in accordance with their electrochemical gradient.
  • Sequence similarities

    Belongs to the potassium channel family. B (Shab) (TC 1.A.1.2) subfamily. Kv2.1/KCNB1 sub-subfamily.
  • Domain

    The segment S4 is probably the voltage-sensor and is characterized by a series of positively charged amino acids at every third position.
    The tail may be important in modulation of channel activity and/or targeting of the channel to specific subcellular compartments.
  • Post-translational
    modifications

    Highly phosphorylated on serine residues in the C-terminal. Differential phosphorylation on a subset of serines allows graded activity-dependent regulation of channel gating. Phosphorylation on Ser-457, Ser-541, Ser-567, Ser-607, Ser-656 and Ser-720 as well as the N-terminal Ser-15 are all regulated by calcineurin-mediated dephosphorylation. Particularly, Ser-607 and Tyr-128 are significant sites of voltage-gated regulation through phosphorylation/ dephosphorylation activities. Tyr-128 can be dephosphorylated by PTPalpha and cyt-PTPepsilon. Phosphorylation levels on Ser-607 are supersensitive to neuronal activity. Phosphorylation on Ser-567 is reduced during postnatal development with low levels at P2 and P5. Levels then increase to reach adult levels by P14. Phosphorylation levels on Ser-564 and Ser-607 are greatly reduced during seizures, by 40% and 85% respectively.
  • Cellular localization

    Membrane.
  • Information by UniProt
  • Database links

  • Alternative names

    • Delayed rectifier potassium channel 1 antibody
    • Delayed rectifier potassium channel Kv2.1 antibody
    • DRK 1 antibody
    • DRK1 antibody
    • h DRK1 K(+) channel antibody
    • h-DRK1 antibody
    • hDRK 1 antibody
    • hDRK1 antibody
    • KCB 1 antibody
    • KCB1 antibody
    • KCNB1 antibody
    • KCNB1_HUMAN antibody
    • KV2.1 antibody
    • Potassium channel protein DRK1 antibody
    • Potassium voltage gated channel shab related subfamily member 1 antibody
    • Potassium voltage-gated channel subfamily B member 1 antibody
    • Voltage-gated potassium channel subunit Kv2.1 antibody
    see all

Images

  • IHC image of Kv2.1/KCNB1 staining in Human Normal Hippocampus formalin fixed paraffin embedded tissue section*, performed on a Leica Bond™ system using the standard protocol F. The section was pre-treated using heat mediated antigen retrieval with sodium citrate buffer (pH6, epitope retrieval solution 1) for 20 mins. The section was then incubated with ab192761, 1µg/ml, for 15 mins at room temperature and detected using an HRP conjugated compact polymer system. DAB was used as the chromogen. The section was then counterstained with haematoxylin and mounted with DPX.

    For other IHC staining systems (automated and non-automated) customers should optimize variable parameters such as antigen retrieval conditions, primary antibody concentration and antibody incubation times.

    *Tissue obtained from the Human Research Tissue Bank, supported by the NIHR Cambridge Biomedical Research Centre

  • All lanes : Anti-Kv2.1/KCNB1 antibody [K89/34] (ab192761) at 1 µg/ml

    Lane 1 : Rat brain normal tissue lysate - total protein (ab29475)
    Lane 2 : Hippocampus (Mouse) Tissue Lysate
    Lane 3 : Human brain tissue lysate - total protein (ab29466)
    Lane 4 : Rat Olfactory Bulb Lysate

    Lysates/proteins at 20 µg per lane.

    Secondary
    All lanes : Goat Anti-Mouse IgG H&L (HRP) preadsorbed (ab97040) at 1/50000 dilution

    Developed using the ECL technique.

    Performed under reducing conditions.

    Predicted band size: 95 kDa
    Observed band size: 98 kDa
    why is the actual band size different from the predicted?


    Exposure time: 20 minutes

References

This product has been referenced in:

  • Corona C  et al. Activating Transcription Factor 4 (ATF4) Regulates Neuronal Activity by Controlling GABABR Trafficking. J Neurosci 38:6102-6113 (2018). Read more (PubMed: 29875265) »
See 1 Publication for this product

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Please note: All products are "FOR RESEARCH USE ONLY. NOT FOR USE IN DIAGNOSTIC PROCEDURES"
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