Anti-Kv4.2/KCND2 antibody [5B11B9] (ab204942)
Key features and details
- Mouse monoclonal [5B11B9] to Kv4.2/KCND2
- Suitable for: WB, Flow Cyt
- Reacts with: Human
- Isotype: IgG1
Overview
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Product name
Anti-Kv4.2/KCND2 antibody [5B11B9]
See all Kv4.2/KCND2 primary antibodies -
Description
Mouse monoclonal [5B11B9] to Kv4.2/KCND2 -
Host species
Mouse -
Tested applications
Suitable for: WB, Flow Cytmore details -
Species reactivity
Reacts with: Human
Predicted to work with: Mouse, Rat, Rabbit -
Immunogen
Recombinant fragment corresponding to Human Kv4.2/KCND2 aa 1-200. (Purified from E.coli).
Database link: Q9NZV8 -
Positive control
- Recombinant Human Kv4.2/KCND2 (aa27-184) protein; Kv4.2/KCND2 (aa27-184)-hIgGFc transfected HEK293 cell lysate; HeLa cells.
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General notes
The Life Science industry has been in the grips of a reproducibility crisis for a number of years. Abcam is leading the way in addressing this with our range of recombinant monoclonal antibodies and knockout edited cell lines for gold-standard validation. Please check that this product meets your needs before purchasing.
If you have any questions, special requirements or concerns, please send us an inquiry and/or contact our Support team ahead of purchase. Recommended alternatives for this product can be found below, along with publications, customer reviews and Q&As
Properties
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Form
Liquid -
Storage instructions
Shipped at 4°C. Store at +4°C short term (1-2 weeks). Upon delivery aliquot. Store at -20°C long term. Avoid freeze / thaw cycle. -
Storage buffer
Preservative: 0.05% Sodium azide
Constituent: 99% PBS -
Concentration information loading...
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Purity
Protein G purified -
Purification notes
Purified from tissue culture supernatant. -
Clonality
Monoclonal -
Clone number
5B11B9 -
Isotype
IgG1 -
Research areas
Associated products
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Compatible Secondaries
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Isotype control
Applications
The Abpromise guarantee
Our Abpromise guarantee covers the use of ab204942 in the following tested applications.
The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.
Application | Abreviews | Notes |
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WB |
1/500 - 1/2000. Predicted molecular weight: 71 kDa.
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Flow Cyt |
1/200 - 1/400.
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Notes |
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WB
1/500 - 1/2000. Predicted molecular weight: 71 kDa. |
Flow Cyt
1/200 - 1/400. |
Target
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Function
Pore-forming (alpha) subunit of voltage-gated rapidly inactivating A-type potassium channels. May contribute to I(To) current in heart and I(Sa) current in neurons. Channel properties are modulated by interactions with other alpha subunits and with regulatory subunits. -
Tissue specificity
Highly expressed throughout the brain. Expression is very low or absent in other tissues. -
Sequence similarities
Belongs to the potassium channel family. D (Shal) (TC 1.A.1.2) subfamily. Kv4.2/KCND2 sub-subfamily. -
Domain
The segment S4 is probably the voltage-sensor and is characterized by a series of positively charged amino acids at every third position. -
Post-translational
modificationsPhosphorylated on serine and threonine residues. -
Cellular localization
Cell membrane. Cell projection > dendrite. Detected in dendrites in cultured hippocampal neurons. Association with KCNIP2 probably enhances cell surface expression. - Information by UniProt
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Database links
- Entrez Gene: 3751 Human
- Entrez Gene: 16508 Mouse
- Entrez Gene: 100008841 Rabbit
- Entrez Gene: 65180 Rat
- Omim: 605410 Human
- SwissProt: Q9NZV8 Human
- SwissProt: Q9Z0V2 Mouse
- SwissProt: P59995 Rabbit
see all -
Alternative names
- KCD2 antibody
- KCND 2 antibody
- KCND2 antibody
see all
Images
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Anti-Kv4.2/KCND2 antibody [5B11B9] (ab204942) at 1/500 dilution + Recombinant Human Kv4.2/KCND2 (aa27-184) protein
Predicted band size: 71 kDaExpected MWt is 42.3 kDa.
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All lanes : Anti-Kv4.2/KCND2 antibody [5B11B9] (ab204942) at 1/500 dilution
Lane 1 : HEK293 cell lysate
Lane 2 : Kv4.2/KCND2 (aa27-184)-hIgGFc transfected HEK293 cell lysate
Predicted band size: 71 kDa -
Flow cytometric analysis of HeLa cells labeling Kv4.2/KCND2 with ab204942 at 1/200 dilution (green), compared to a negative control (red).
Datasheets and documents
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Datasheet download
References (0)
ab204942 has not yet been referenced specifically in any publications.