Anti-Kv4.2/KCND2 antibody (ab123543)
- Datasheet
- References (1)
- Protocols
Overview
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Product name
Anti-Kv4.2/KCND2 antibody
See all Kv4.2/KCND2 primary antibodies -
Description
Rabbit polyclonal to Kv4.2/KCND2 -
Host species
Rabbit -
Tested applications
Suitable for: WB, ICC/IFmore details -
Species reactivity
Reacts with: Mouse, Rat
Predicted to work with: Rabbit, Chicken, Cow, Dog, Human, Pig, Macaque monkey, Chinese hamster, Orangutan
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Immunogen
Synthetic peptide corresponding to Human Kv4.2/KCND2 aa 600 to the C-terminus conjugated to keyhole limpet haemocyanin.
(Peptide available asab138806) -
Positive control
- This antibody gave a positive signal in Mouse and Rat Heart tissue lysates. This antibody gave a positive result in IF in the following Formaldehyde fixed cell line: SKNSH
Properties
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Form
Liquid -
Storage instructions
Shipped at 4°C. Store at +4°C short term (1-2 weeks). Upon delivery aliquot. Store at -20°C or -80°C. Avoid freeze / thaw cycle. -
Storage buffer
pH: 7.40
Preservative: 0.02% Sodium azide
Constituent: PBS
Note: Batches of this product that have a concentration < 1mg/ml may have BSA added as a stabilising agent. If you would like information about the formulation of a specific lot, please contact our scientific support team who will be happy to help. -
Concentration information loading... -
Purity
Immunogen affinity purified -
Clonality
Polyclonal -
Isotype
IgG -
Research areas
Associated products
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Compatible Secondaries
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Isotype control
Applications
Our Abpromise guarantee covers the use of ab123543 in the following tested applications.
The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.
| Application | Abreviews | Notes |
|---|---|---|
| WB | Use a concentration of 1 µg/ml. Detects a band of approximately 74 kDa (predicted molecular weight: 70 kDa). | |
| ICC/IF | Use a concentration of 5 µg/ml. |
Target
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Function
Pore-forming (alpha) subunit of voltage-gated rapidly inactivating A-type potassium channels. May contribute to I(To) current in heart and I(Sa) current in neurons. Channel properties are modulated by interactions with other alpha subunits and with regulatory subunits. -
Tissue specificity
Highly expressed throughout the brain. Expression is very low or absent in other tissues. -
Sequence similarities
Belongs to the potassium channel family. D (Shal) (TC 1.A.1.2) subfamily. Kv4.2/KCND2 sub-subfamily. -
Domain
The segment S4 is probably the voltage-sensor and is characterized by a series of positively charged amino acids at every third position. -
Post-translational
modificationsPhosphorylated on serine and threonine residues. -
Cellular localization
Cell membrane. Cell projection > dendrite. Detected in dendrites in cultured hippocampal neurons. Association with KCNIP2 probably enhances cell surface expression. - Information by UniProt
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Database links
- Entrez Gene: 3751 Human
- Entrez Gene: 16508 Mouse
- Entrez Gene: 100008841 Rabbit
- Entrez Gene: 65180 Rat
- Omim: 605410 Human
- SwissProt: Q9NZV8 Human
- SwissProt: Q9Z0V2 Mouse
- SwissProt: P59995 Rabbit
see all -
Alternative names
- KCD2 antibody
- KCND 2 antibody
- KCND2 antibody
see all
Images
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Western blot - Anti-Kv4.2/KCND2 antibody (ab123543)All lanes : Anti-Kv4.2/KCND2 antibody (ab123543) at 1 µg/ml
Lane 1 : Heart (Mouse) Tissue Lysate
Lane 2 : Heart (Rat) Tissue Lysate
Lysates/proteins at 10 µg per lane.
Secondary
All lanes : Goat Anti-Rabbit IgG H&L (HRP) preadsorbed (ab97080) at 1/5000 dilution
Developed using the ECL technique.
Performed under reducing conditions.
Predicted band size: 70 kDa
Observed band size: 74 kDa why is the actual band size different from the predicted?
Additional bands at: 90 kDa. We are unsure as to the identity of these extra bands.
Exposure time: 4 minutes -
Immunocytochemistry/ Immunofluorescence - Anti-Kv4.2/KCND2 antibody (ab123543)ab123543 stained SKNSH cells. The cells were 4% formaldehyde fixed (10 min) and then incubated in 1%BSA / 10% normal goat serum / 0.3M glycine in 0.1% PBS-Tween for 1h to permeabilise the cells and block non-specific protein-protein interactions. The cells were then incubated with the antibody ab123543 at 5µg/ml overnight at +4°C. The secondary antibody (green) was DyLight® 488 goat anti- rabbit (ab96899) IgG (H+L) used at a 1/1000 dilution for 1h. Alexa Fluor® 594 WGA was used to label plasma membranes (red) at a 1/200 dilution for 1h. DAPI was used to stain the cell nuclei (blue) at a concentration of 1.43µM.
References
This product has been referenced in:
- Panama BK et al. Reductions in the Cardiac Transient Outward K+ Current Ito Caused by Chronic ß-Adrenergic Receptor Stimulation Are Partly Rescued by Inhibition of Nuclear Factor ?B. J Biol Chem 291:4156-65 (2016). Read more (PubMed: 26742842) »
