Overview

  • Product name
  • Description
    Rabbit polyclonal to Kv4.2/KCND2
  • Host species
    Rabbit
  • Tested applications
    Suitable for: WB, ICC/IF, IP, ELISAmore details
  • Species reactivity
    Reacts with: Rat
    Predicted to work with: Mouse, Rabbit, Human, Ferret
  • Immunogen

    Synthetic peptide corresponding to Rat Kv4.2/KCND2 aa 23-43 conjugated to keyhole limpet haemocyanin.
    Sequence:

    ASGPMPAPPRQERKRTQDALI

  • Positive control
    • Transfected HEK293 cells containing Kv4.2/KCND2.2 cDNA.

Properties

Applications

Our Abpromise guarantee covers the use of ab16719 in the following tested applications.

The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.

Application Abreviews Notes
WB 1/1000. Detects a band of approximately 80 kDa (predicted molecular weight: 70 kDa).
ICC/IF Use at an assay dependent dilution.
IP Use at an assay dependent dilution.
ELISA Use at an assay dependent dilution.

Target

  • Function
    Pore-forming (alpha) subunit of voltage-gated rapidly inactivating A-type potassium channels. May contribute to I(To) current in heart and I(Sa) current in neurons. Channel properties are modulated by interactions with other alpha subunits and with regulatory subunits.
  • Tissue specificity
    Highly expressed throughout the brain. Expression is very low or absent in other tissues.
  • Sequence similarities
    Belongs to the potassium channel family. D (Shal) (TC 1.A.1.2) subfamily. Kv4.2/KCND2 sub-subfamily.
  • Domain
    The segment S4 is probably the voltage-sensor and is characterized by a series of positively charged amino acids at every third position.
  • Post-translational
    modifications
    Phosphorylated on serine and threonine residues.
  • Cellular localization
    Cell membrane. Cell projection > dendrite. Detected in dendrites in cultured hippocampal neurons. Association with KCNIP2 probably enhances cell surface expression.
  • Information by UniProt
  • Database links
  • Alternative names
    • KCD2 antibody
    • KCND 2 antibody
    • KCND2 antibody
    • KCND2_HUMAN antibody
    • KIAA1044 antibody
    • MGC119702 antibody
    • MGC119703 antibody
    • Potassium voltage gated channel Shal related subfamily member 2 antibody
    • Potassium voltage-gated channel subfamily D member 2 antibody
    • RK 5 antibody
    • RK5 antibody
    • Voltage gated potassium channel Kv4.2 antibody
    • Voltage gated potassium channel subunit Kv4.2 antibody
    • Voltage sensitive potassium channel antibody
    • Voltage-gated potassium channel subunit Kv4.2 antibody
    see all

Images

  • ab16719 detecting Kv4.2/KCND2 by ELISA. The sample was purified from rat neuronal cells and blocked with 1% milk for 1 hour at 18°C. The primary antibody was used at 1/1000 dilution, and incubated with sample for 1 hour at 18°C.       

    See Abreview

  • All lanes : Anti-Kv4.2/KCND2 antibody (ab16719)

    Lanes 1-3 : Lysate prepared from total cellular protein (intracellular + surface)
    Lanes 4-6 : Lysate prepared from biotinylated, surface-expressed channels (as supported by the absence of the intracellular protein synaptophysin)

    Predicted band size: 70 kDa



    ab16719 used in Western Blot analysis. The positive control used was prepared from cultured rat hippocampal slices. Lanes containing surface channels were loaded with 3 times the protein loaded for total protein analysis.

References

This product has been referenced in:
  • Yang CH  et al. A critical role for protein tyrosine phosphatase nonreceptor type 5 in determining individual susceptibility to develop stress-related cognitive and morphological changes. J Neurosci 32:7550-62 (2012). Read more (PubMed: 22649233) »
  • Noam Y  et al. Trafficking and surface expression of hyperpolarization-activated cyclic nucleotide-gated channels in hippocampal neurons. J Biol Chem 285:14724-36 (2010). WB ; Rat . Read more (PubMed: 20215108) »
See all 3 Publications for this product

Customer reviews and Q&As

1-2 of 2 Abreviews or Q&A

Abcam guarantees this product to work in the species/application used in this Abreview.
Application
ELISA
Sample
Rat Purified protein (neuronal)
Specification
neuronal
Blocking step
Milk as blocking agent for 1 hour(s) and 0 minute(s) · Concentration: 1% · Temperature: 18°C
Type
Indirect

Abcam user community

Verified customer

Submitted May 06 2009

Application
Western blot
Sample
Escherichia coli Purified protein (E.Coli)
Gel Running Conditions
Reduced Denaturing (gradient 4-12%)
Loading amount
0.05 µg
Specification
E.Coli
Blocking step
Milk as blocking agent for 1 hour(s) and 0 minute(s) · Concentration: 5% · Temperature: 4°C

Abcam user community

Verified customer

Submitted Jan 09 2009

Please note: All products are "FOR RESEARCH USE ONLY AND ARE NOT INTENDED FOR DIAGNOSTIC OR THERAPEUTIC USE"

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