Product nameAnti-Kv4.2/KCND2 antibody
See all Kv4.2/KCND2 primary antibodies
DescriptionRabbit polyclonal to Kv4.2/KCND2
Tested applicationsSuitable for: WB, ICC/IF, IP, ELISAmore details
Species reactivityReacts with: Rat
Predicted to work with: Mouse, Rabbit, Human, Ferret
- Transfected HEK293 cells containing Kv4.2/KCND2.2 cDNA.
Storage instructionsShipped at 4°C. Upon delivery aliquot and store at -20°C. Avoid freeze / thaw cycles.
Storage bufferpH: 7.40
Preservative: 0.08% Sodium azide
Concentration information loading...
PurityAmmonium Sulphate Precipitation
Our Abpromise guarantee covers the use of ab16719 in the following tested applications.
The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.
|WB||1/1000. Detects a band of approximately 80 kDa (predicted molecular weight: 70 kDa).|
|ICC/IF||Use at an assay dependent dilution.|
|IP||Use at an assay dependent dilution.|
|ELISA||Use at an assay dependent dilution.|
FunctionPore-forming (alpha) subunit of voltage-gated rapidly inactivating A-type potassium channels. May contribute to I(To) current in heart and I(Sa) current in neurons. Channel properties are modulated by interactions with other alpha subunits and with regulatory subunits.
Tissue specificityHighly expressed throughout the brain. Expression is very low or absent in other tissues.
Sequence similaritiesBelongs to the potassium channel family. D (Shal) (TC 1.A.1.2) subfamily. Kv4.2/KCND2 sub-subfamily.
DomainThe segment S4 is probably the voltage-sensor and is characterized by a series of positively charged amino acids at every third position.
modificationsPhosphorylated on serine and threonine residues.
Cellular localizationCell membrane. Cell projection > dendrite. Detected in dendrites in cultured hippocampal neurons. Association with KCNIP2 probably enhances cell surface expression.
- Information by UniProt
- KCD2 antibody
- KCND 2 antibody
- KCND2 antibody
ab16719 detecting Kv4.2/KCND2 by ELISA. The sample was purified from rat neuronal cells and blocked with 1% milk for 1 hour at 18°C. The primary antibody was used at 1/1000 dilution, and incubated with sample for 1 hour at 18°C.
All lanes : Anti-Kv4.2/KCND2 antibody (ab16719)
Lanes 1-3 : Lysate prepared from total cellular protein (intracellular + surface)
Lanes 4-6 : Lysate prepared from biotinylated, surface-expressed channels (as supported by the absence of the intracellular protein synaptophysin)
Predicted band size: 70 kDa
ab16719 used in Western Blot analysis. The positive control used was prepared from cultured rat hippocampal slices. Lanes containing surface channels were loaded with 3 times the protein loaded for total protein analysis.
This product has been referenced in:
- Yang CH et al. A critical role for protein tyrosine phosphatase nonreceptor type 5 in determining individual susceptibility to develop stress-related cognitive and morphological changes. J Neurosci 32:7550-62 (2012). Read more (PubMed: 22649233) »
- Noam Y et al. Trafficking and surface expression of hyperpolarization-activated cyclic nucleotide-gated channels in hippocampal neurons. J Biol Chem 285:14724-36 (2010). WB ; Rat . Read more (PubMed: 20215108) »