Overview

  • Product name

    Anti-Kv4.2/KCND2 antibody [EP982Y]
    See all Kv4.2/KCND2 primary antibodies
  • Description

    Rabbit monoclonal [EP982Y] to Kv4.2/KCND2
  • Host species

    Rabbit
  • Tested applications

    Suitable for: WB, IHC-P, Flow Cytmore details
    Unsuitable for: ICC or IP
  • Species reactivity

    Reacts with: Human
  • Immunogen

    Synthetic peptide within Human Kv4.2/KCND2 (C terminal). The exact sequence is proprietary.

  • Positive control

    • Human brain lysate; Human brain tissue.
  • General notes

    Our RabMAb® technology is a patented hybridoma-based technology for making rabbit monoclonal antibodies. For details on our patents, please refer to RabMab® patents.

Properties

Applications

Our Abpromise guarantee covers the use of ab46797 in the following tested applications.

The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.

Application Abreviews Notes
WB 1/1000. Detects a band of approximately 80 kDa.
IHC-P Use at an assay dependent concentration. Perform heat mediated antigen retrieval with citrate buffer pH 6 before commencing with IHC staining protocol.
Flow Cyt 1/30.

ab172730 - Rabbit monoclonal IgG, is suitable for use as an isotype control with this antibody.

  • Application notes
    Is unsuitable for ICC or IP.
  • Target

    • Function

      Pore-forming (alpha) subunit of voltage-gated rapidly inactivating A-type potassium channels. May contribute to I(To) current in heart and I(Sa) current in neurons. Channel properties are modulated by interactions with other alpha subunits and with regulatory subunits.
    • Tissue specificity

      Highly expressed throughout the brain. Expression is very low or absent in other tissues.
    • Sequence similarities

      Belongs to the potassium channel family. D (Shal) (TC 1.A.1.2) subfamily. Kv4.2/KCND2 sub-subfamily.
    • Domain

      The segment S4 is probably the voltage-sensor and is characterized by a series of positively charged amino acids at every third position.
    • Post-translational
      modifications

      Phosphorylated on serine and threonine residues.
    • Cellular localization

      Cell membrane. Cell projection > dendrite. Detected in dendrites in cultured hippocampal neurons. Association with KCNIP2 probably enhances cell surface expression.
    • Information by UniProt
    • Database links

    • Alternative names

      • KCD2 antibody
      • KCND 2 antibody
      • KCND2 antibody
      • KCND2_HUMAN antibody
      • KIAA1044 antibody
      • MGC119702 antibody
      • MGC119703 antibody
      • Potassium voltage gated channel Shal related subfamily member 2 antibody
      • Potassium voltage-gated channel subfamily D member 2 antibody
      • RK 5 antibody
      • RK5 antibody
      • Voltage gated potassium channel Kv4.2 antibody
      • Voltage gated potassium channel subunit Kv4.2 antibody
      • Voltage sensitive potassium channel antibody
      • Voltage-gated potassium channel subunit Kv4.2 antibody
      see all

    Images

    • Anti-Kv4.2/KCND2 antibody [EP982Y] (ab46797) at 1/1000 dilution + Human brain lysate at 10 µg

      Secondary
      Goat anti-rabbit HRP at 1/2000 dilution

      Observed band size: 80 kDa
      why is the actual band size different from the predicted?
      Additional bands at: 25 kDa. We are unsure as to the identity of these extra bands.

    References

    This product has been referenced in:

    • Cacace R  et al. Loss of DPP6 in neurodegenerative dementia: a genetic player in the dysfunction of neuronal excitability. Acta Neuropathol 137:901-918 (2019). WB ; Human . Read more (PubMed: 30874922) »
    • Aronica E  et al. Expression and localization of voltage dependent potassium channel Kv4.2 in epilepsy associated focal lesions. Neurobiol Dis 36:81-95 (2009). Read more (PubMed: 19596445) »
    See all 2 Publications for this product

    Customer reviews and Q&As

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    Please note: All products are "FOR RESEARCH USE ONLY. NOT FOR USE IN DIAGNOSTIC PROCEDURES"
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