Product nameAnti-Kv4.2/KCND2 antibody [EP982Y]
See all Kv4.2/KCND2 primary antibodies
DescriptionRabbit monoclonal [EP982Y] to Kv4.2/KCND2
Tested applicationsSuitable for: WB, IHC-P, Flow Cytmore details
Unsuitable for: ICC or IP
Species reactivityReacts with: Human
Synthetic peptide within Human Kv4.2/KCND2 (C terminal). The exact sequence is proprietary.
- Human brain lysate; Human brain tissue.
Our RabMAb® technology is a patented hybridoma-based technology for making rabbit monoclonal antibodies. For details on our patents, please refer to RabMab® patents.
Storage instructionsShipped at 4°C. Store at -20°C. Stable for 12 months at -20°C.
Storage bufferpH: 7.20
Preservative: 0.01% Sodium azide
Constituents: 9% PBS, 40% Glycerol, 0.05% BSA, 50% Tissue culture supernatant
PurityTissue culture supernatant
Our Abpromise guarantee covers the use of ab46797 in the following tested applications.
The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.
|WB||1/1000. Detects a band of approximately 80 kDa.|
|IHC-P||Use at an assay dependent concentration. Perform heat mediated antigen retrieval with citrate buffer pH 6 before commencing with IHC staining protocol.|
ab172730 - Rabbit monoclonal IgG, is suitable for use as an isotype control with this antibody.
FunctionPore-forming (alpha) subunit of voltage-gated rapidly inactivating A-type potassium channels. May contribute to I(To) current in heart and I(Sa) current in neurons. Channel properties are modulated by interactions with other alpha subunits and with regulatory subunits.
Tissue specificityHighly expressed throughout the brain. Expression is very low or absent in other tissues.
Sequence similaritiesBelongs to the potassium channel family. D (Shal) (TC 1.A.1.2) subfamily. Kv4.2/KCND2 sub-subfamily.
DomainThe segment S4 is probably the voltage-sensor and is characterized by a series of positively charged amino acids at every third position.
modificationsPhosphorylated on serine and threonine residues.
Cellular localizationCell membrane. Cell projection > dendrite. Detected in dendrites in cultured hippocampal neurons. Association with KCNIP2 probably enhances cell surface expression.
- Information by UniProt
- KCD2 antibody
- KCND 2 antibody
- KCND2 antibody
Anti-Kv4.2/KCND2 antibody [EP982Y] (ab46797) at 1/1000 dilution + Human brain lysate at 10 µg
Goat anti-rabbit HRP at 1/2000 dilution
Observed band size: 80 kDa why is the actual band size different from the predicted?
Additional bands at: 25 kDa. We are unsure as to the identity of these extra bands.
This product has been referenced in:
- Cacace R et al. Loss of DPP6 in neurodegenerative dementia: a genetic player in the dysfunction of neuronal excitability. Acta Neuropathol 137:901-918 (2019). WB ; Human . Read more (PubMed: 30874922) »
- Aronica E et al. Expression and localization of voltage dependent potassium channel Kv4.2 in epilepsy associated focal lesions. Neurobiol Dis 36:81-95 (2009). Read more (PubMed: 19596445) »