Overview

  • Product name

  • Description

    Rabbit polyclonal to Kv4.3/KCND3
  • Host species

    Rabbit
  • Tested applications

    Suitable for: IHC-P, IHC-Fr, WBmore details
  • Species reactivity

    Reacts with: Human
    Predicted to work with: Mouse, Rat
  • Immunogen

    Synthetic peptide corresponding to Human Kv4.3/KCND3 (N terminal).

  • General notes

     This product was previously labelled as Kv4.3

     

Properties

Applications

Our Abpromise guarantee covers the use of ab65794 in the following tested applications.

The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.

Application Abreviews Notes
IHC-P Use at an assay dependent concentration.
IHC-Fr Use at an assay dependent concentration.
WB 1/200 - 1/2000. Predicted molecular weight: 73 kDa.

Target

  • Function

    Pore-forming (alpha) subunit of voltage-gated rapidly inactivating A-type potassium channels. May contribute to I(To) current in heart and I(Sa) current in neurons. Channel properties are modulated by interactions with other alpha subunits and with regulatory subunits.
  • Tissue specificity

    Highly expressed in heart and brain, in particular in cortex, cerebellum, amygdala and caudate nucleus. Detected at lower levels in liver, skeletal muscle, kidney and pancreas. Isoform 1 predominates in most tissues. Isoform 1 and isoform 2 are detected at similar levels in brain, skeletal muscle and pancreas.
  • Sequence similarities

    Belongs to the potassium channel family. D (Shal) (TC 1.A.1.2) subfamily. Kv4.3/KCND3 sub-subfamily.
  • Domain

    The segment S4 is probably the voltage-sensor and is characterized by a series of positively charged amino acids at every third position.
  • Cellular localization

    Membrane. Cell membrane > sarcolemma.
  • Information by UniProt
  • Database links

  • Alternative names

    • KCND 3 antibody
    • KCND 3L antibody
    • KCND 3S antibody
    • Kcnd3 antibody
    • KCND3_HUMAN antibody
    • KCND3L antibody
    • KCND3S antibody
    • KSHIVB antibody
    • Kv 4.3 antibody
    • MGC14203 antibody
    • MGC142037 antibody
    • OTTHUMP00000063948 antibody
    • Potassium ioinic channel Kv4.3 antibody
    • Potassium voltage gated channel Shal related subfamily member 3 antibody
    • Potassium voltage gated channel subfamily D member 3 antibody
    • Potassium voltage-gated channel subfamily D member 3 antibody
    • Sha1 related potassium channel Kv4.3 antibody
    • Voltage gated K+ channel antibody
    • Voltage gated potassium channel Kv4.3 antibody
    • Voltage gated potassium channel subunit Kv4.3 antibody
    • Voltage-gated potassium channel subunit Kv4.3 antibody
    see all

References

This product has been referenced in:

  • Matkovich SJ  et al. MicroRNA-133a protects against myocardial fibrosis and modulates electrical repolarization without affecting hypertrophy in pressure-overloaded adult hearts. Circ Res 106:166-75 (2010). WB ; Human . Read more (PubMed: 19893015) »
See 1 Publication for this product

Customer reviews and Q&As

1-4 of 4 Abreviews or Q&A

Answer

I understand your concern.

We have not seen any complaints of this type with this antibody. Although I cannot guarantee that your following experiment will not yield similar results, I have seen this type of response with other product before. Usually type of response does occur as a reaction to the blocking buffer but using freshly made buffers and reagents would also be advisable. Do you pre-rinse your membrane prior to transfer with methanol?

I hope that this information is helpful. Please let me know if you have any questions or there are other ways that Abcam may help you meet your research goals.

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Answer

Thank you for providing this additional information.

It seems apparent that the antibody is reacting to something on this blot. It is usually the case in instances like this that there is reaction to the proteins in your blocking buffer. I would strongly recommend changing your blocking and incubation buffers from milk to acasein-based blocking solution or to BSA. We find that the casin based buffers give the best results in our studies and do not react with the antibodies like this. Our10X Blocking Buffer can be found at the following link:https://www.abcam.com/10X-Blocking-Buffer-ab126587.html

I would also recommend running a "no primary" control as well to ensure that it is not the secondary which is reacting with something.

Finally, I would suggest using a nitrocellulose membrane to determine if this is a reaction with the PVDF.

Please let me know how these suggestions work for you and if you have any questions or concerns.

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Answer

Thank you for providing information.

I am sorry to hear of the issues that you have been experiencing.In order to help with our investigation of this case before deciding how to proceed, I would appreciate if you are able to provide the details of the protocol that you have used. Any troubleshooting steps you may have taken as well as images would also be very helpful. This information will also be helpful to our quality monitoring.

If we are unable to rectify this issue and this product has been purchased within the last 6 months,I will be pleased to provide a free of charge replacement or credit note.

Thank you for your time. I look forward to hearing from you with the requested information and hope we can resolve this case as soon as possible.

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Answer

Thank you for contacting Abcam. I am sorry to hear that ab65794 has not been working for you. I will be glad to help you find an alternative antibody. To help determine the antibody that will best suit your needs, could you tell me what tissue/species you are working in. Also is there any application that you are primarily interested in eg WB, IHC-P, IP, ICC. I look forward to hearing back from you and helping to find an antibody for you.

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