Key features and details
- Rabbit polyclonal to Kyphoscoliosis peptidase
- Suitable for: ICC/IF, IHC-P, WB
- Reacts with: Human
- Isotype: IgG
Product nameAnti-Kyphoscoliosis peptidase antibody
DescriptionRabbit polyclonal to Kyphoscoliosis peptidase
Tested applicationsSuitable for: ICC/IF, IHC-P, WBmore details
Species reactivityReacts with: Human
Recombinant fragment corresponding to Human Kyphoscoliosis peptidase aa 23-170.
Database link: Q8NBH2
- WB: HeLa, MCF7 and U-87 MG whole cell lysates. IHC-P: Human skeletal muscle tissue. ICC/IF: HeLa cells.
Previously labelled as KY.
Storage instructionsShipped at 4°C. Store at +4°C short term (1-2 weeks). Upon delivery aliquot. Store at -20°C long term. Avoid freeze / thaw cycle.
Storage bufferpH: 7.40
Constituents: 50% Glycerol (glycerin, glycerine), PBS, 0.03% Proclin 300
Concentration information loading...
PurityProtein G purified
Purification notesPurity >95%
Our Abpromise guarantee covers the use of ab236741 in the following tested applications.
The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.
|ICC/IF||1/50 - 1/200.|
|IHC-P||1/20 - 1/200.|
|WB||1/500 - 1/5000. Predicted molecular weight: 64 kDa.|
FunctionProbable cytoskeleton-associated protease required for normal muscle growth. Involved in function, maturation and stabilization of the neuromuscular junction. May act by cleaving muscle-specific proteins such as FLNC.
Sequence similaritiesBelongs to the transglutaminase-like superfamily.
Cellular localizationCytoplasm > cytoskeleton. Cytoplasm > myofibril > sarcomere > Z-disk.
- Information by UniProt
- KY antibody
- KY_HUMAN antibody
- Kyphoscoliosis peptidase antibody
All lanes : Anti-Kyphoscoliosis peptidase antibody (ab236741) at 1/500 dilution
Lane 1 : HeLa (Human epithelial cell line from cervix adenocarcinoma) whole cell lysate
Lane 2 : MCF7 (Human breast adenocarcinoma cell line) whole cell lysate
Lane 3 : U-87 MG (Human glioblastoma-astrocytoma epithelial cell line) whole cell lysate
All lanes : Goat polyclonal to rabbit IgG at 1/50000 dilution
Predicted band size: 64 kDa
Paraffin-embedded human skeletal muscle tissue stained for Kyphoscoliosis peptidase using ab236741 at 1/100 dilution in immunohistochemical analysis.
HeLa (Human epithelial cell line from cervix adenocarcinoma) cells stained for Kyphoscoliosis peptidase (green) using ab236741 at 1/133 dilution in ICC/IF. Secondary antibody is an Alexa-Fluor® 488-conjugated Goat Anti-Rabbit IgG (H+L). The nuclear counterstain is DAPI (blue).
The cells were fixed in 4% formaldehyde, permeabilized tissue using 0.2% Triton X-100 and blocked in 10% normal goat serum. The cells were then incubated with the antibody overnight at 4°C.
To our knowledge, customised protocols are not required for this product. Please try the standard protocols listed below and let us know how you get on.
ab236741 has not yet been referenced specifically in any publications.