Overview

  • Product name

    Anti-Kyphoscoliosis peptidase antibody
  • Description

    Rabbit polyclonal to Kyphoscoliosis peptidase
  • Host species

    Rabbit
  • Tested applications

    Suitable for: ICC/IF, IHC-P, WBmore details
  • Species reactivity

    Reacts with: Human
  • Immunogen

    Recombinant fragment corresponding to Human Kyphoscoliosis peptidase aa 23-170.
    Database link: Q8NBH2

  • Positive control

    • WB: HeLa, MCF7 and U-87 MG whole cell lysates. IHC-P: Human skeletal muscle tissue. ICC/IF: HeLa cells.
  • General notes

    Previously labelled as KY. 

Properties

Applications

Our Abpromise guarantee covers the use of ab236741 in the following tested applications.

The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.

Application Abreviews Notes
ICC/IF 1/50 - 1/200.
IHC-P 1/20 - 1/200.
WB 1/500 - 1/5000. Predicted molecular weight: 64 kDa.

Target

  • Function

    Probable cytoskeleton-associated protease required for normal muscle growth. Involved in function, maturation and stabilization of the neuromuscular junction. May act by cleaving muscle-specific proteins such as FLNC.
  • Sequence similarities

    Belongs to the transglutaminase-like superfamily.
  • Cellular localization

    Cytoplasm > cytoskeleton. Cytoplasm > myofibril > sarcomere > Z-disk.
  • Information by UniProt
  • Database links

  • Alternative names

    • KY antibody
    • KY_HUMAN antibody
    • Kyphoscoliosis peptidase antibody

Images

  • All lanes : Anti-Kyphoscoliosis peptidase antibody (ab236741) at 1/500 dilution

    Lane 1 : HeLa (Human epithelial cell line from cervix adenocarcinoma) whole cell lysate
    Lane 2 : MCF7 (Human breast adenocarcinoma cell line) whole cell lysate
    Lane 3 : U-87 MG (Human glioblastoma-astrocytoma epithelial cell line) whole cell lysate

    Secondary
    All lanes : Goat polyclonal to rabbit IgG at 1/50000 dilution

    Predicted band size: 64 kDa

  • Paraffin-embedded human skeletal muscle tissue stained for Kyphoscoliosis peptidase using ab236741 at 1/100 dilution in immunohistochemical analysis.

  • HeLa (Human epithelial cell line from cervix adenocarcinoma) cells stained for Kyphoscoliosis peptidase (green) using ab236741 at 1/133 dilution in ICC/IF. Secondary antibody is an Alexa-Fluor® 488-conjugated Goat Anti-Rabbit IgG (H+L). The nuclear counterstain is DAPI (blue).

    The cells were fixed in 4% formaldehyde, permeabilized tissue using 0.2% Triton X-100 and blocked in 10% normal goat serum. The cells were then incubated with the antibody overnight at 4°C.

References

ab236741 has not yet been referenced specifically in any publications.

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