Recombinant

Recombinant Anti-l + S Afadin antibody [A7L9H48] (ab203569)

Overview

  • Product name

    Anti-l + S Afadin antibody [A7L9H48]
    See all l + S Afadin primary antibodies
  • Description

    Rabbit monoclonal [A7L9H48] to l + S Afadin
  • Host species

    Rabbit
  • Tested applications

    Suitable for: WB, IHC-Pmore details
  • Species reactivity

    Reacts with: Mouse
    Predicted to work with: Rat, Human
  • Immunogen

    Synthetic peptide corresponding to Mouse l + S Afadin aa 1805-1820 (C terminal).
    Sequence:

    KASRKLTELENELNTK


    Database link: Q9QZQ1

  • Positive control

    • Mouse hyppocampus tissue; Mouse hyppocampus tissue lysate

Properties

Applications

Our Abpromise guarantee covers the use of ab203569 in the following tested applications.

The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.

Application Abreviews Notes
WB Use a concentration of 1 - 2 µg/ml. Predicted molecular weight: 207 kDa.

Do not boil samples.

IHC-P Use a concentration of 1 - 2 µg/ml.

Target

  • Function

    Belongs to an adhesion system, probably together with the E-cadherin-catenin system, which plays a role in the organization of homotypic, interneuronal and heterotypic cell-cell adherens junctions (AJs). Nectin- and actin-filament-binding protein that connects nectin to the actin cytoskeleton.
  • Involvement in disease

    Note=A chromosomal aberration involving MLLT4 is associated with acute leukemias. Translocation t(6;11)(q27;q23) with MLL/HRX. The result is a rogue activator protein.
  • Sequence similarities

    Contains 1 dilute domain.
    Contains 1 FHA domain.
    Contains 1 PDZ (DHR) domain.
    Contains 2 Ras-associating domains.
  • Domain

    The PDZ/DHR domain interacts with the C-terminus of nectin and the Pro-rich C-terminus domain interacts with F-actin.
  • Cellular localization

    Cell junction > adherens junction. Not found at cell-matrix AJs.
  • Information by UniProt
  • Database links

  • Alternative names

    • AF6 antibody
    • AFAD_HUMAN antibody
    • Afadin antibody
    • ALL1-fused gene from chromosome 6 protein antibody
    • Mllt4 antibody
    • Protein Af-6 antibody
    • Protein AF6 antibody
    see all

Images

  • Anti-l + S Afadin antibody [A7L9H48] (ab203569) at 1 µg/ml + Mouse hyppocampus tissue lysate (unboiled)

    Predicted band size: 207 kDa

  • Immunohistochemical analysis of Mouse hyppocampus tissue labeling l + S Afadin using ab203569 at 1 μg/ml.

References

This product has been referenced in:

  • Shah B  et al. Rap1 GTPases Are Master Regulators of Neural Cell Polarity in the Developing Neocortex. Cereb Cortex N/A:N/A (2016). ICC/IF ; Mouse . Read more (PubMed: 26733533) »
See 1 Publication for this product

Customer reviews and Q&As

Application
Immunohistochemistry (Frozen sections)
Sample
Mouse Tissue sections (Embryonic brain tissue section)
Permeabilization
Yes - triton X
Specification
Embryonic brain tissue section
Blocking step
Serum as blocking agent for 1 hour(s) and 0 minute(s) · Concentration: 1% · Temperature: 25°C
Fixative
Paraformaldehyde

Dr. Bhavin Shah

Verified customer

Submitted Oct 13 2015

Please note: All products are "FOR RESEARCH USE ONLY. NOT FOR USE IN DIAGNOSTIC PROCEDURES"
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