Recombinant Anti-AF6 antibody [A7L9H48] (ab203569)
Key features and details
- Produced recombinantly (animal-free) for high batch-to-batch consistency and long term security of supply
- Rabbit monoclonal [A7L9H48] to AF6
- Suitable for: WB, IHC-P
- Reacts with: Mouse
Overview
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Product name
Anti-AF6 antibody [A7L9H48]
See all AF6 primary antibodies -
Description
Rabbit monoclonal [A7L9H48] to AF6 -
Host species
Rabbit -
Tested applications
Suitable for: WB, IHC-Pmore details -
Species reactivity
Reacts with: Mouse
Predicted to work with: Rat, Human -
Immunogen
Synthetic peptide corresponding to Mouse Afadin aa 1800 to the C-terminus (C terminal).
Database link: Q9QZQ1 -
Positive control
- Mouse hyppocampus tissue; Mouse hyppocampus tissue lysate
Properties
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Form
Liquid -
Storage instructions
Shipped at 4°C. Store at +4°C short term (1-2 weeks). Upon delivery aliquot. Store at -20°C long term. Avoid freeze / thaw cycle. -
Storage buffer
Preservative: 0.09% Sodium azide
Constituent: 99% PBS -
Concentration information loading...
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Purity
Protein A purified -
Clonality
Monoclonal -
Clone number
A7L9H48 -
Isotype
IgG -
Research areas
Associated products
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Compatible Secondaries
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Isotype control
Applications
The Abpromise guarantee
Our Abpromise guarantee covers the use of ab203569 in the following tested applications.
The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.
Application | Abreviews | Notes |
---|---|---|
WB |
Use a concentration of 1 - 2 µg/ml. Predicted molecular weight: 207 kDa.
Do not boil samples. |
|
IHC-P |
Use a concentration of 1 - 2 µg/ml.
|
Notes |
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WB
Use a concentration of 1 - 2 µg/ml. Predicted molecular weight: 207 kDa. Do not boil samples. |
IHC-P
Use a concentration of 1 - 2 µg/ml. |
Target
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Function
Belongs to an adhesion system, probably together with the E-cadherin-catenin system, which plays a role in the organization of homotypic, interneuronal and heterotypic cell-cell adherens junctions (AJs). Nectin- and actin-filament-binding protein that connects nectin to the actin cytoskeleton. -
Involvement in disease
Note=A chromosomal aberration involving MLLT4 is associated with acute leukemias. Translocation t(6;11)(q27;q23) with MLL/HRX. The result is a rogue activator protein. -
Sequence similarities
Contains 1 dilute domain.
Contains 1 FHA domain.
Contains 1 PDZ (DHR) domain.
Contains 2 Ras-associating domains. -
Domain
The PDZ/DHR domain interacts with the C-terminus of nectin and the Pro-rich N-terminus domain interacts with F-actin. -
Cellular localization
Cell junction > adherens junction. Not found at cell-matrix AJs. - Information by UniProt
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Database links
- Entrez Gene: 4301 Human
- Entrez Gene: 17356 Mouse
- Entrez Gene: 26955 Rat
- Omim: 159559 Human
- SwissProt: P55196 Human
- SwissProt: Q9QZQ1 Mouse
- SwissProt: O35889 Rat
- Unigene: 614974 Human
see all -
Alternative names
- AF6 antibody
- AFAD_HUMAN antibody
- Afadin antibody
see all
Images
Protocols
Datasheets and documents
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SDS download
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Datasheet download
References (1)
ab203569 has been referenced in 1 publication.
- Shah B et al. Rap1 GTPases Are Master Regulators of Neural Cell Polarity in the Developing Neocortex. Cereb Cortex N/A:N/A (2016). ICC/IF ; Mouse . PubMed: 26733533