Key features and details
- Produced recombinantly (animal-free) for high batch-to-batch consistency and long term security of supply
- Rabbit monoclonal [A7L9H48] to l + S Afadin
- Suitable for: WB, IHC-P
- Reacts with: Mouse
Product nameAnti-l + S Afadin antibody [A7L9H48]
See all l + S Afadin primary antibodies
DescriptionRabbit monoclonal [A7L9H48] to l + S Afadin
Tested applicationsSuitable for: WB, IHC-Pmore details
Species reactivityReacts with: Mouse
Predicted to work with: Rat, Human
- Mouse hyppocampus tissue; Mouse hyppocampus tissue lysate
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Abcam is leading the way with our range of recombinant antibodies, knockout-validated antibodies and knockout cell lines, all of which support improved reproducibility.
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In preparation for this, we have started to update the applications & species that this product is Abpromise guaranteed for.
We are also updating the applications & species that this product has been “predicted to work with,” however this information is not covered by our Abpromise guarantee.
Applications & species from publications and Abreviews that have not been tested in our own labs or in those of our suppliers are not covered by the Abpromise guarantee.
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Storage instructionsShipped at 4°C. Store at +4°C short term (1-2 weeks). Upon delivery aliquot. Store at -20°C long term. Avoid freeze / thaw cycle.
Storage bufferPreservative: 0.09% Sodium azide
Constituent: 99% PBS
Concentration information loading...
PurityProtein A purified
Our Abpromise guarantee covers the use of ab203569 in the following tested applications.
The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.
|WB||Use a concentration of 1 - 2 µg/ml. Predicted molecular weight: 207 kDa.
Do not boil samples.
|IHC-P||Use a concentration of 1 - 2 µg/ml.|
FunctionBelongs to an adhesion system, probably together with the E-cadherin-catenin system, which plays a role in the organization of homotypic, interneuronal and heterotypic cell-cell adherens junctions (AJs). Nectin- and actin-filament-binding protein that connects nectin to the actin cytoskeleton.
Involvement in diseaseNote=A chromosomal aberration involving MLLT4 is associated with acute leukemias. Translocation t(6;11)(q27;q23) with MLL/HRX. The result is a rogue activator protein.
Sequence similaritiesContains 1 dilute domain.
Contains 1 FHA domain.
Contains 1 PDZ (DHR) domain.
Contains 2 Ras-associating domains.
DomainThe PDZ/DHR domain interacts with the C-terminus of nectin and the Pro-rich C-terminus domain interacts with F-actin.
Cellular localizationCell junction > adherens junction. Not found at cell-matrix AJs.
- Information by UniProt
- AF6 antibody
- AFAD_HUMAN antibody
- Afadin antibody
ab203569 has been referenced in 1 publication.
- Shah B et al. Rap1 GTPases Are Master Regulators of Neural Cell Polarity in the Developing Neocortex. Cereb Cortex N/A:N/A (2016). ICC/IF ; Mouse . PubMed: 26733533