Overview

  • Product name

    Anti-l Afadin antibody [3]
  • Description

    Mouse monoclonal [3] to l Afadin
  • Host species

    Mouse
  • Tested applications

    Suitable for: WB, IP, IHC-Fr, ELISAmore details
  • Species reactivity

    Reacts with: Mouse, Rat, Dog, Human
  • Immunogen

    Peptide: KASRKLTELENELNTK, corresponding to amino acids 1814-1829 of Rat l Afadin.

Properties

Applications

Our Abpromise guarantee covers the use of ab90809 in the following tested applications.

The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.

Application Abreviews Notes
WB 1/50. Predicted molecular weight: 143 kDa.
IP Use at an assay dependent concentration.
IHC-Fr 1/50.
ELISA Use at an assay dependent concentration.

Target

  • Function

    Belongs to an adhesion system, probably together with the E-cadherin-catenin system, which plays a role in the organization of homotypic, interneuronal and heterotypic cell-cell adherens junctions (AJs). Nectin- and actin-filament-binding protein that connects nectin to the actin cytoskeleton.
  • Involvement in disease

    Note=A chromosomal aberration involving MLLT4 is associated with acute leukemias. Translocation t(6;11)(q27;q23) with MLL/HRX. The result is a rogue activator protein.
  • Sequence similarities

    Contains 1 dilute domain.
    Contains 1 FHA domain.
    Contains 1 PDZ (DHR) domain.
    Contains 2 Ras-associating domains.
  • Domain

    The PDZ/DHR domain interacts with the C-terminus of nectin and the Pro-rich N-terminus domain interacts with F-actin.
  • Cellular localization

    Cell junction > adherens junction. Not found at cell-matrix AJs.
  • Information by UniProt
  • Database links

  • Alternative names

    • AF6 antibody
    • AFAD_HUMAN antibody
    • Afadin antibody
    • ALL1-fused gene from chromosome 6 protein antibody
    • Mllt4 antibody
    • Protein Af-6 antibody
    • Protein AF6 antibody
    see all

References

This product has been referenced in:

  • Moniz LS  et al. Phosphoproteomic comparison of Pik3ca and Pten signalling identifies the nucleotidase NT5C as a novel AKT substrate. Sci Rep 7:39985 (2017). WB ; Mouse . Read more (PubMed: 28059163) »
  • Poon CE  et al. Nectin-3 Is Increased in the Cell Junctions of the Uterine Epithelium at Implantation. Reprod Sci 23:1580-1592 (2016). Read more (PubMed: 27217376) »
See all 3 Publications for this product

Customer reviews and Q&As

Application
Western blot
Sample
Human Cell lysate - whole cell (MDA-MB-231 cells)
Loading amount
100 µg
Specification
MDA-MB-231 cells
Gel Running Conditions
Reduced Denaturing (10%)
Blocking step
Milk as blocking agent for 8 hour(s) and 0 minute(s) · Concentration: 10% · Temperature: 6-8°C

Abcam user community

Verified customer

Submitted May 11 2011

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