Key features and details
- Rabbit polyclonal to L-Alanine
- Suitable for: IHC-FoFr, ICC, IHC-Fr, Immunomicroscopy
- Reacts with: Species independent
- Isotype: IgG
Product nameAnti-L-Alanine antibody
DescriptionRabbit polyclonal to L-Alanine
SpecificityThe antibody is calibrated against a spectrum of antigens to assure hapten selectivity. No measurable cross-reactivity (<1:1000) was detected against alanine in peptides or proteins. Fixed tissue cross-reactivity was tested with known targets at the recommended dilution. No measurable glutaraldehyde-fixed tissue cross-reactivity (<1:1000) was detected against D/L-aspartate, D/L-arginine, agmatine, guanidine, gamma-aminobutyrate, L-citrulline, L-cysteine, D/L-glutamate, D/L-glutamine, glutathione, glycine, L-lysine, L-ornithine, L-serine, taurine, L-threonine, L-tryptophan, L-tyrosine. Significant cross-reactivity (1:10) was detected against beta-alanine.
Tested applicationsSuitable for: IHC-FoFr, ICC, IHC-Fr, Immunomicroscopymore details
Species reactivityReacts with: Species independent
Chemical/ Small Molecule by a Glutaraldehyde linker.
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One factor contributing to the crisis is the use of antibodies that are not suitable. This can lead to misleading results and the use of incorrect data informing project assumptions and direction. To help address this challenge, we have introduced an application and species grid on our primary antibody datasheets to make it easy to simplify identification of the right antibody for your needs.
Learn more here.
Storage instructionsShipped at 4°C. Store at +4°C short term (1-2 weeks). Store at +4°C. Do Not Freeze.
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The Abpromise guarantee
Our Abpromise guarantee covers the use of ab9436 in the following tested applications.
The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.
Recommended user dilution: 1/100
True Dilution at User Dilution: 1/8000
High-performance thin section immunostaining using silver-intensified immunogold or fluorescence detection.
Enhanced detection is possible using streptavidin detection.
EM applications with 10-40 nm gold GAR IgGs or gold streptavidin.
OPTIMAL FIXATION: 0.1-2.5% glutaraldehyde,1% formaldehyde using HPI protocol (The antisera targets the glutaraldehyde conjugate of the hapten).
MINIMAL GLUTARALDEHYDE: 0.1% using HPI
The hapten is osmium tolerant (deosmication required), therefore the antisera can be used on conventional post-embedding electron microscope immunostaining.
All procedures may be carried out at room temperature. Exact dilutions for all applications cannot be predicted, but it is unlikely that deviations from the calibrated levels will be needed. Dilutions are optimized for antigen detection over a 2 log unit range.
The product is optimized for HPI/EHPI with gold or fluorescence detection using etched plastic sections. Filter diluted reagents with 0.2 mm syringe filters before use on EM grids. Enzyme-linked visualizations can be used but will compress the signal dynamic range and are less sensitive.
Use with frozen or vibratome sections is possible but will not yield optimal images as IgGs penetrate aldehyde cross-linked tissue poorly and most amino acids are present at such high levels that prozone effects occur. Use in whole mounts is not recommended for similar reasons.
ab9436 has been referenced in 1 publication.
- Sun D et al. Metabolic and functional profiling of the normal rat retina. J Comp Neurol 505:92-113 (2007). IHC ; Rat . PubMed: 17729258