Product nameL-Amino Acid Assay Kit
Sample typeCell culture supernatant, Urine, Serum, Plasma, Other biological fluids, Tissue Extracts
Sensitivity<= 40 µM
Assay time0h 30m
L-Amino Acid Assay Kit ab65347 is used measure the level of L-amino acids in biological samples. There is no requirement for sample pretreatment or purification.
The L-amino acid(s) level can be quantified using fluorometric (at Ex/Em = 535/587 nm) or colorimetric (at λ = 570 nm) methods in 96-well plates.
Amino acid assay protocol summary:
- add reaction mix to sample and standard wells
- incubate for 30 min
- analyze with microplate reader
L-Amino acids are the most essential elements in biology. Accurately quantitating L-amino acids in body fluids or purified samples may provide valuable information for diagnostic or basic research studies.
Storage instructionsStore at -20°C. Please refer to protocols.
Components Identifier 100 tests L-Amino Acid Assay Buffer WM 1 x 25ml L-Amino Acid Enzyme Mix Green 1 vial L-amino Acid Probe Red 1 x 0.2ml L-Amino Acid Standard (4 nmol/µl) Yellow 1 x 300µl
RelevanceAmino acids perform various important functions for the body and serve as the materials for the body's cells, hormones, and enzymes. Of the standard amino acids, all by glycine can exist in either of the two optical isomers, called D and L enantiomers, which are mirror images of each other. The L-amino acids represent the vast majority of amino acids found in proteins during translation in the ribosome, and are therefore the most essential element in biology and metabolic functions.
This product has been referenced in:
- Modvig IM et al. Peptone-mediated glucagon-like peptide-1 secretion depends on intestinal absorption and activation of basolaterally located Calcium-Sensing Receptors. Physiol Rep 7:e14056 (2019). Read more (PubMed: 31020803) »
- Posada IMD et al. Opposite feedback from mTORC1 to H-ras and K-ras4B downstream of SREBP1. Sci Rep 7:8944 (2017). Read more (PubMed: 28827765) »