• Product name
    L-Amino Acid Assay Kit
  • Sample type
    Cell culture supernatant, Urine, Serum, Plasma, Other biological fluids, Tissue Extracts
  • Assay type
  • Sensitivity
    <= 40 µM
  • Assay time
    0h 30m
  • Product overview

    Abcam's L-Amino Acid Assay Kit provides a convenient means for directly detecting L-amino acid in biological samples. There is no requirement for sample pretreatment or purification when using this kit. The L-amino acid(s) level can be quantified using fluorometric (at Ex/Em = 535/587 nm) or colorimetric (at ? = 570 nm) methods in 96-well plates.
    Visit our FAQs page for tips and troubleshooting.

  • Notes

    L-Amino acids are the most essential elements in biology. Accurately quantitating L-amino acids in body fluids or purified samples may provide valuable information for diagnostic or basic research studies.


  • Storage instructions
    Store at -20°C. Please refer to protocols.
  • Components Identifier 100 tests
    L-Amino Acid Assay Buffer WM 1 x 25ml
    L-Amino Acid Enzyme Mix Green 1 vial
    L-amino Acid Probe Red 1 x 0.2ml
    L-Amino Acid Standard (4 nmol/µl) Yellow 1 x 300µl
  • Research areas
  • Relevance
    Amino acids perform various important functions for the body and serve as the materials for the body's cells, hormones, and enzymes. Of the standard amino acids, all by glycine can exist in either of the two optical isomers, called D and L enantiomers, which are mirror images of each other. The L-amino acids represent the vast majority of amino acids found in proteins during translation in the ribosome, and are therefore the most essential element in biology and metabolic functions.


  • Standard curve (fluorimetrical): mean of duplicates (+/-) with background subtracted

  • Standard curve (colorimetrical): mean of duplicates (+/-SD) with background readings subtracted

  • L-Amino Acid Standard Curve.



This product has been referenced in:

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Customer reviews and Q&As

ab83374 (Branched Chain Amino Acid Assay Kit). We have not tested this kit with cell or tissue lysates yet but only with fluids like Cell culture supernatant, Urine, Serum, Plasma and can therefore not provide more than a general protocol below. Read More

Merci de nous avoir contactés.

Le laboratoire m'a confirmé que nous n'avons malheureusement pas de données expérimentales pour ces kits sur des lysats musculaires.

Désolé de ne pas pouvoir vou...

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I am afraid that this is proprietary information which cannot be disclosed.

We apologize for any inconvenience this may cause.

I general, werecommend using several doses of your serum sample to ensure the readings are within the standard curve range. In essence, the dose range selection depends on your expectation of the L-amino-acid concentration in your samples.


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Thank you for your enquiry.

I am sorry to confirm we do not currently have specific data in terms of what reagents and at what concentrations would interfere with this assay. Theoretically chemicals at high concentrations and ionic detergent...

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Thank you for your phone call today. I have gotten in touch with the production laboratory, and I was told that the kit only detects free L-amino acids. I hope this is helpful but please let me know if you have further questions.


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